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The map label for this gene is purT [H]

Identifier: 29141475

GI number: 29141475

Start: 1083999

End: 1085177

Strand: Reverse

Name: purT [H]

Synonym: t0994

Alternate gene names: 29141475

Gene position: 1085177-1083999 (Counterclockwise)

Preceding gene: 29141480

Following gene: 29141471

Centisome position: 22.65

GC content: 56.83

Gene sequence:

>1179_bases
ATGACGCTATTAGGCACTGCGCTACGTCCGGCAGCAACGCGGGTGATGTTATTAGGGGCAGGTGAATTGGGAAAAGAGGT
GGCGATTGAATGCCAACGCCTGGGGATCGAGGTTATCGCCGTCGATCGCTATCCTGATGCTCCCGCCATGCATGTGGCTC
ACCGTTCACACGTCATTAATATGCTGGACGGCGAGGCGCTACGTCATGTGATTACAGAGGAAAAACCGCATTATATCGTG
CCGGAAATAGAAGCGATCGCCACCGATACGCTGCGCGAGCTGGAGGGCGAAGGGCTGAATGTCGTGCCTTGCGCCCGTGC
AACGCAGCTCACGATGAACCGCGAAGGGATCCGTCGCCTGGCCGCAGAAGAATTAGGTCTGCCGACATCGACGTATCGCT
TTGCCGACAGTGAGGCCAGTTTTCATGATGCGGTAGCCGCAGTGGGTTTTCCTTGCATCGTCAAACCGGTCATGAGCTCT
TCCGGCAAAGGCCAGAGCTTTATCCGCTCGGCCGAACAGCTCGCGCAGGCATGGGAGTATGCTCAACAGGGCGGACGCGC
TGGCGCGGGTCGCGTGATTGTGGAAGGCGTGGTTAAATTTGATTTTGAAATTACGCTGCTCACCGTTAGCGCCGTCGATG
GCGTGCATTTCTGCGCGCCGGTCGGTCATCGTCAGCAAGATGGTGACTATCGCGAATCCTGGCAACCACAGCAGATGAGC
GAACTGGCGCTGAAGCGGGCGCAAGAGATTGCTCGTCATGTGGTACTGGCGTTAGGCGGTCATGGTCTGTTCGGCGTTGA
ACTCTTCGTCTGTGGCGATGAAGTCATTTTCAGCGAAGTCTCCCCTCGCCCGCACGATACCGGAATGGTCACGTTGATTT
CTCAGGATCTTTCTGAGTTTGCGCTGCATGTGCGCGCCTTTCTGGGAATGCCCATAGGCGCTATTCGCCAGTATGGTCCC
GCTGCCTCGGCCGTGATTCTGCCGCAGCTTACCAGTCAAAATGTGACGTTTGATGATGTACACGCGGCGGTAGGGGCCGG
AGTGCAGGTACGGCTGTTTGGTAAGCCTGAGATCGACGGCACCCGTCGTCTTGGTGTAGCGTTAGCGACAGGTGAAAACG
TTGAAGAAGCGGTGATAAGAGCGAAAAAGGCCGCCAGCCGCGTGACGGTAAAAGGCTAA

Upstream 100 bases:

>100_bases
TACAGTATATTGCAAGGCGGCAAGAAAAGCAATTTATTCGAAAAGACATACGCAAACGTTTTCGTATATACTGCGCGCAG
GTAAATAAGGGGACAGAAAT

Downstream 100 bases:

>100_bases
TGCGCCATCGCCTGATGGCGACGTTAGCACATTTTTATCAGGCCTGCCTGATCGCCACTCGTAAGCCCGATAAGCAAGCG
CCATCGGGCATTTAACGGCT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTLLGTALRPAATRVMLLGAGELGKEVAIECQRLGIEVIAVDRYPDAPAMHVAHRSHVINMLDGEALRHVITEEKPHYIV
PEIEAIATDTLRELEGEGLNVVPCARATQLTMNREGIRRLAAEELGLPTSTYRFADSEASFHDAVAAVGFPCIVKPVMSS
SGKGQSFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFCAPVGHRQQDGDYRESWQPQQMS
ELALKRAQEIARHVVLALGGHGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGMPIGAIRQYGP
AASAVILPQLTSQNVTFDDVHAAVGAGVQVRLFGKPEIDGTRRLGVALATGENVEEAVIRAKKAASRVTVKG

Sequences:

>Translated_392_residues
MTLLGTALRPAATRVMLLGAGELGKEVAIECQRLGIEVIAVDRYPDAPAMHVAHRSHVINMLDGEALRHVITEEKPHYIV
PEIEAIATDTLRELEGEGLNVVPCARATQLTMNREGIRRLAAEELGLPTSTYRFADSEASFHDAVAAVGFPCIVKPVMSS
SGKGQSFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFCAPVGHRQQDGDYRESWQPQQMS
ELALKRAQEIARHVVLALGGHGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGMPIGAIRQYGP
AASAVILPQLTSQNVTFDDVHAAVGAGVQVRLFGKPEIDGTRRLGVALATGENVEEAVIRAKKAASRVTVKG
>Mature_391_residues
TLLGTALRPAATRVMLLGAGELGKEVAIECQRLGIEVIAVDRYPDAPAMHVAHRSHVINMLDGEALRHVITEEKPHYIVP
EIEAIATDTLRELEGEGLNVVPCARATQLTMNREGIRRLAAEELGLPTSTYRFADSEASFHDAVAAVGFPCIVKPVMSSS
GKGQSFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFCAPVGHRQQDGDYRESWQPQQMSE
LALKRAQEIARHVVLALGGHGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGMPIGAIRQYGPA
ASAVILPQLTSQNVTFDDVHAAVGAGVQVRLFGKPEIDGTRRLGVALATGENVEEAVIRAKKAASRVTVKG

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=87.5, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1786733, Length=245, Percent_Identity=24.8979591836735, Blast_Score=65, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324702, Length=304, Percent_Identity=23.6842105263158, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42167; Mature: 42036

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLGTALRPAATRVMLLGAGELGKEVAIECQRLGIEVIAVDRYPDAPAMHVAHRSHVIN
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHH
MLDGEALRHVITEEKPHYIVPEIEAIATDTLRELEGEGLNVVPCARATQLTMNREGIRRL
HHCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHCCHHHHHHH
AAEELGLPTSTYRFADSEASFHDAVAAVGFPCIVKPVMSSSGKGQSFIRSAEQLAQAWEY
HHHHHCCCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFCAPVGHRQQDGDYRESWQPQQMS
HHHCCCCCCCHHHEEEEHEEEEEEEEEEEECCCCCCEECCCCCCCCCCCHHHCCCHHHHH
ELALKRAQEIARHVVLALGGHGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEF
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHCCCCCCCCCCEEEEHHHHHHHH
ALHVRAFLGMPIGAIRQYGPAASAVILPQLTSQNVTFDDVHAAVGAGVQVRLFGKPEIDG
HHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCCC
TRRLGVALATGENVEEAVIRAKKAASRVTVKG
CCEEEEEEECCCCHHHHHHHHHHHHCCEECCC
>Mature Secondary Structure 
TLLGTALRPAATRVMLLGAGELGKEVAIECQRLGIEVIAVDRYPDAPAMHVAHRSHVIN
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHH
MLDGEALRHVITEEKPHYIVPEIEAIATDTLRELEGEGLNVVPCARATQLTMNREGIRRL
HHCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHCCHHHHHHH
AAEELGLPTSTYRFADSEASFHDAVAAVGFPCIVKPVMSSSGKGQSFIRSAEQLAQAWEY
HHHHHCCCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFCAPVGHRQQDGDYRESWQPQQMS
HHHCCCCCCCHHHEEEEHEEEEEEEEEEEECCCCCCEECCCCCCCCCCCHHHCCCHHHHH
ELALKRAQEIARHVVLALGGHGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEF
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHCCCCCCCCCCEEEEHHHHHHHH
ALHVRAFLGMPIGAIRQYGPAASAVILPQLTSQNVTFDDVHAAVGAGVQVRLFGKPEIDG
HHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCCC
TRRLGVALATGENVEEAVIRAKKAASRVTVKG
CCEEEEEEECCCCHHHHHHHHHHHHCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA