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The map label for this gene is cbiF

Identifier: 29141341

GI number: 29141341

Start: 938667

End: 939440

Strand: Direct

Name: cbiF

Synonym: t0845

Alternate gene names: 29141341

Gene position: 938667-939440 (Clockwise)

Preceding gene: 29141340

Following gene: 29141342

Centisome position: 19.59

GC content: 57.36

Gene sequence:

>774_bases
ATGTCAGAGACATTTGATCCACGCTGTGTATGGTTCGTCGGCGCCGGGCCGGGCGATCGCGAGCTGATCACGCTTAAAGG
CTACCGCCTGCTGCAACAGGCGCAGGTAGTTATCTATGCCGGTTCGCTGATCAATACCGAACTGCTGGATTACTGCCCCG
CGCAGGCAGAACGCTACGACAGCGCCGAACTGCATCTTGAGCAGATTATCGAGCTGATGGCCGCGGGCGTGAAAGCGGGA
AAAACGGTGGTGCGGTTGCAAACGGGCGATGTCTCGCTGTACGGCTCCGTGCGTGAGCAAGGCGAAGAGTTAACCCGCCG
TGGTATCGACTGGCAGGTAGTGCCTGGCGTCAGCGCGTTTCTCGGCGCTGCGGCGGAGCTGGGTGTGGAATATACCGTAC
CGGAGGTATCGCAGAGTCTCATTATTACCCGTCTGGAAGGGCGTACGCCAGTACCGGCGCGCGAACAACTGGAGGCGTTC
GCCAGTCACCAGACGTCGATGGCGATTTATCTCTCCGTTCAGCGTATTCATCGGGTGGCGGAACGTCTCATTGCCGGCGG
TTATCCGGCAACTACGCCTGTCGCAGTCATCTATAAGGCTACCTGGCCGGAAAGCCAGACCGTTCGCGGCACGTTAGCGG
ATATTAGCGACAAAGTACGTGATGCTGGCATCCGTAAGACGGCGCTAATCCTGGTCGGGAATTTTCTGGGTAAGGAGTAT
CACTACTCAAGACTTTATGCAGCGGACTTTAGCCATGAATACCGTAAAGCCTGA

Upstream 100 bases:

>100_bases
GTACAGCTTCAGCTTTCATCGTTAACGCCGCTGGGGGCGGGACACTATTTCAAACCGAACAACCCTGTTTTTGTTATAGC
CTGTCAGAAGGAAGAAAACC

Downstream 100 bases:

>100_bases
ATCCATTGCGTTGTTTTGCCTGACGCCCGGCGGCGTGGCGCTGGCAAAGAGACTCGCCGCGATGCTGCCGTTAACCTGCT
TTACCAGTGAAAAACTGCGG

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSETFDPRCVWFVGAGPGDRELITLKGYRLLQQAQVVIYAGSLINTELLDYCPAQAERYDSAELHLEQIIELMAAGVKAG
KTVVRLQTGDVSLYGSVREQGEELTRRGIDWQVVPGVSAFLGAAAELGVEYTVPEVSQSLIITRLEGRTPVPAREQLEAF
ASHQTSMAIYLSVQRIHRVAERLIAGGYPATTPVAVIYKATWPESQTVRGTLADISDKVRDAGIRKTALILVGNFLGKEY
HYSRLYAADFSHEYRKA

Sequences:

>Translated_257_residues
MSETFDPRCVWFVGAGPGDRELITLKGYRLLQQAQVVIYAGSLINTELLDYCPAQAERYDSAELHLEQIIELMAAGVKAG
KTVVRLQTGDVSLYGSVREQGEELTRRGIDWQVVPGVSAFLGAAAELGVEYTVPEVSQSLIITRLEGRTPVPAREQLEAF
ASHQTSMAIYLSVQRIHRVAERLIAGGYPATTPVAVIYKATWPESQTVRGTLADISDKVRDAGIRKTALILVGNFLGKEY
HYSRLYAADFSHEYRKA
>Mature_256_residues
SETFDPRCVWFVGAGPGDRELITLKGYRLLQQAQVVIYAGSLINTELLDYCPAQAERYDSAELHLEQIIELMAAGVKAGK
TVVRLQTGDVSLYGSVREQGEELTRRGIDWQVVPGVSAFLGAAAELGVEYTVPEVSQSLIITRLEGRTPVPAREQLEAFA
SHQTSMAIYLSVQRIHRVAERLIAGGYPATTPVAVIYKATWPESQTVRGTLADISDKVRDAGIRKTALILVGNFLGKEYH
YSRLYAADFSHEYRKA

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=244, Percent_Identity=29.9180327868852, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322922, Length=232, Percent_Identity=22.8448275862069, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBIF_SALTI (P0A2H0)

Other databases:

- EMBL:   AL627273
- EMBL:   AE014613
- RefSeq:   NP_456580.1
- RefSeq:   NP_804683.1
- ProteinModelPortal:   P0A2H0
- SMR:   P0A2H0
- PRIDE:   P0A2H0
- GeneID:   1070124
- GeneID:   1248572
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t0845
- KEGG:   sty:STY2234
- HOGENOM:   HBG730212
- OMA:   TVIITRP
- ProtClustDB:   PRK15473
- BioCyc:   SENT209261:T0845-MONOMER
- BioCyc:   SENT220341:STY2234-MONOMER
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- TIGRFAMs:   TIGR01465

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: 2.1.1.133

Molecular weight: Translated: 28377; Mature: 28245

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETFDPRCVWFVGAGPGDRELITLKGYRLLQQAQVVIYAGSLINTELLDYCPAQAERYD
CCCCCCCCEEEEEECCCCCCEEEEEHHHHHHHHCEEEEEECCHHHHHHHHHCCCHHHHCC
SAELHLEQIIELMAAGVKAGKTVVRLQTGDVSLYGSVREQGEELTRRGIDWQVVPGVSAF
CHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEHHHHHHHHHHHHCCCCEEECCCHHHH
LGAAAELGVEYTVPEVSQSLIITRLEGRTPVPAREQLEAFASHQTSMAIYLSVQRIHRVA
HHHHHHCCCEEECHHHHHHEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHH
ERLIAGGYPATTPVAVIYKATWPESQTVRGTLADISDKVRDAGIRKTALILVGNFLGKEY
HHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHH
HYSRLYAADFSHEYRKA
HHHEEEECCCCHHHHCC
>Mature Secondary Structure 
SETFDPRCVWFVGAGPGDRELITLKGYRLLQQAQVVIYAGSLINTELLDYCPAQAERYD
CCCCCCCEEEEEECCCCCCEEEEEHHHHHHHHCEEEEEECCHHHHHHHHHCCCHHHHCC
SAELHLEQIIELMAAGVKAGKTVVRLQTGDVSLYGSVREQGEELTRRGIDWQVVPGVSAF
CHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEHHHHHHHHHHHHCCCCEEECCCHHHH
LGAAAELGVEYTVPEVSQSLIITRLEGRTPVPAREQLEAFASHQTSMAIYLSVQRIHRVA
HHHHHHCCCEEECHHHHHHEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHH
ERLIAGGYPATTPVAVIYKATWPESQTVRGTLADISDKVRDAGIRKTALILVGNFLGKEY
HHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHH
HYSRLYAADFSHEYRKA
HHHEEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504