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The map label for this gene is cbiE

Identifier: 29141339

GI number: 29141339

Start: 937510

End: 938115

Strand: Direct

Name: cbiE

Synonym: t0843

Alternate gene names: 29141339

Gene position: 937510-938115 (Clockwise)

Preceding gene: 29141338

Following gene: 29141340

Centisome position: 19.56

GC content: 58.09

Gene sequence:

>606_bases
ATGCTAACGGTCGTGGGAATGGGGCCCGCTGGACGGCATTTGATGACGCCTGCGGCGCTGGAAGCGATAGACCACGCCGA
TGCGCTGGCAGGCGGTAAACGGCATTTGGCGCAGTTTCCGGCGTTTGGCGGCGAACGATTTACGCTGGGCGCGGATATTG
GCGCGTTGTTGTCATGGATTGCCGCCCGTCGGGATAAAGGGATCGTGGTGCTGGCCTCCGGCGATCCGCTGTTTTATGGC
ATCGGTACACGCCTGGTGGCGCATTTTGGTATCGAACAGGTTCGCATTATCCCTGGCATCAGCGCCGTACAGTATCTGTG
CGCACAGGCTGGAATTGATATGAATGACATGTGGTTGACCAGTAGCCACGGTCGTTGCGTCAGCTTCGAGCAGTTGGCGA
ACCATCGCAAAGTGGCGATGGTCACGGATGCCCGCTGCGGGCCGCGGGAAATCGCGCGGGAACTGGTCGCGCGTGGTAAA
GGGCATCGCTTGATGGTGATTGGTGAAAATCTGGCGATGGAAAATGAGCGGATCCACTGGCTACCCGTCAGTGCGGTCAA
CGCCGACTATGAAATGAATGCAGTGGTGATCCTGGATGAAAGATGA

Upstream 100 bases:

>100_bases
TTACCAAAACGCCGCCTGTCTGCGACGCCATCCTGTTTTCTTTTGATAACCATATTCTCGGCAGTAATCGTCCCGTCGAC
GAGATTGCTAAGGAGCTGCA

Downstream 100 bases:

>100_bases
GCTTTTTCTGCGCGGCGAGAACGTGCCAATGACCAAAGAGGCGGTACGGGCGCTGGCACTGTCGAAACTTGAGCTCCACC
GCGCCAGTCACCTGATTGAC

Product: cobalt-precorrin-6Y C(5)-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 8X; CO2

Alternate protein names: Cobalt-precorrin-6 methyltransferase; Cobalt-precorrin-6Y methylase

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MLTVVGMGPAGRHLMTPAALEAIDHADALAGGKRHLAQFPAFGGERFTLGADIGALLSWIAARRDKGIVVLASGDPLFYG
IGTRLVAHFGIEQVRIIPGISAVQYLCAQAGIDMNDMWLTSSHGRCVSFEQLANHRKVAMVTDARCGPREIARELVARGK
GHRLMVIGENLAMENERIHWLPVSAVNADYEMNAVVILDER

Sequences:

>Translated_201_residues
MLTVVGMGPAGRHLMTPAALEAIDHADALAGGKRHLAQFPAFGGERFTLGADIGALLSWIAARRDKGIVVLASGDPLFYG
IGTRLVAHFGIEQVRIIPGISAVQYLCAQAGIDMNDMWLTSSHGRCVSFEQLANHRKVAMVTDARCGPREIARELVARGK
GHRLMVIGENLAMENERIHWLPVSAVNADYEMNAVVILDER
>Mature_201_residues
MLTVVGMGPAGRHLMTPAALEAIDHADALAGGKRHLAQFPAFGGERFTLGADIGALLSWIAARRDKGIVVLASGDPLFYG
IGTRLVAHFGIEQVRIIPGISAVQYLCAQAGIDMNDMWLTSSHGRCVSFEQLANHRKVAMVTDARCGPREIARELVARGK
GHRLMVIGENLAMENERIHWLPVSAVNADYEMNAVVILDER

Specific function: Probably catalyzes the methylation of C-5 in cobalt- precorrin-6Y to form cobalt-precorrin-7W-a

COG id: COG2241

COG function: function code H; Precorrin-6B methylase 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBIE_SALTI (P0A2H2)

Other databases:

- EMBL:   AL627273
- EMBL:   AE014613
- RefSeq:   NP_456582.1
- RefSeq:   NP_804681.1
- ProteinModelPortal:   P0A2H2
- SMR:   P0A2H2
- PRIDE:   P0A2H2
- GeneID:   1070122
- GeneID:   1248574
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t0843
- KEGG:   sty:STY2236
- HOGENOM:   HBG646061
- OMA:   SIWLTDK
- ProtClustDB:   PRK05787
- BioCyc:   SENT209261:T0843-MONOMER
- BioCyc:   SENT220341:STY2236-MONOMER
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR012818
- Gene3D:   G3DSA:3.40.1010.10
- TIGRFAMs:   TIGR02467

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: 2.1.1.132

Molecular weight: Translated: 21779; Mature: 21779

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTVVGMGPAGRHLMTPAALEAIDHADALAGGKRHLAQFPAFGGERFTLGADIGALLSWI
CEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEECCCHHHHHHHH
AARRDKGIVVLASGDPLFYGIGTRLVAHFGIEQVRIIPGISAVQYLCAQAGIDMNDMWLT
HHCCCCCEEEEECCCEEEEEHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCEEEE
SSHGRCVSFEQLANHRKVAMVTDARCGPREIARELVARGKGHRLMVIGENLAMENERIHW
CCCCCEEEHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCEECCCCEEEE
LPVSAVNADYEMNAVVILDER
EEEEEECCCCCCEEEEEEECC
>Mature Secondary Structure
MLTVVGMGPAGRHLMTPAALEAIDHADALAGGKRHLAQFPAFGGERFTLGADIGALLSWI
CEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEECCCHHHHHHHH
AARRDKGIVVLASGDPLFYGIGTRLVAHFGIEQVRIIPGISAVQYLCAQAGIDMNDMWLT
HHCCCCCEEEEECCCEEEEEHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCEEEE
SSHGRCVSFEQLANHRKVAMVTDARCGPREIARELVARGKGHRLMVIGENLAMENERIHW
CCCCCEEEHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCEECCCCEEEE
LPVSAVNADYEMNAVVILDER
EEEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 6Y

Specific reaction: 2 S-adenosyl-L-methionine + precorrin-6Y = 2 S-adenosyl-L-homocysteine + precorrin-8X + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504