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The map label for this gene is hisF [H]

Identifier: 29141296

GI number: 29141296

Start: 891001

End: 891777

Strand: Reverse

Name: hisF [H]

Synonym: t0796

Alternate gene names: 29141296

Gene position: 891777-891001 (Counterclockwise)

Preceding gene: 29141297

Following gene: 29141295

Centisome position: 18.61

GC content: 54.95

Gene sequence:

>777_bases
ATGCTGGCAAAACGTATAATTCCGTGTCTGGACGTTCGTGATGGTCAGGTGGTAAAAGGCGTACAGTTTCGCAACCATGA
GATCATTGGCGATATCGTTCCGCTGGCCAAACGCTATGCCGACGAAGGCGCGGACGAACTGGTGTTCTATGACATTACCG
CCTCCAGCGATGGTCGCGTAGTCGATAAAAGCTGGGTAGCGCGCGTTGCCGAGGTGATCGACATTCCGTTTTGTGTAGCG
GGCGGTATCCGGTCAATTGACGACGCCGCCAAAATTCTCTCTTTCGGGGCGGATAAGATCTCTATCAACTCCCCTGCACT
GGCTGACCCAACGCTGATTACCCGTCTGGCTGACCGTTTTGGCGTGCAGTGCATTGTCGTCGGGATTGATACCTGGTTTG
ACGACGCCACGGGGAAATATCATGTTAACCAGTATACCGGCGATGAAAACCGTACCCGCGTGACGCAGTGGGAGACGCTG
GACTGGGTGCAAGAGGTACAACAGCGCGGCGCGGGGGAAATCGTCCTGAATATGATGAACCAGGACGGCGTGCGTAACGG
TTATGATCTGACGCAGTTGAAAAAAGTCCGTGACGTTTGCCGCGTGCCGCTGATCGCCTCCGGCGGCGCGGGCACGATGG
AACACTTTCTTGAGGCATTCCGTGATGCCGATGTCGACGGCGCGCTTGCCGCCTCCGTTTTTCACAAGCAAATCATCAAT
ATTGGCGAATTAAAAGCGTACCTGGCAGGCCAGGGCGTGGAGATCAGGATATGTTAA

Upstream 100 bases:

>100_bases
ATATCGATGATATTGCCGCCCTGCGCGGCACCGGCGTGCGCGGCGTGATTGTCGGACGCGCGCTGTTGGAAGGGAAATTT
ACCGTTAAGGAGGCCATCCA

Downstream 100 bases:

>100_bases
CAGAGCAACAACGCCGCGAGCTGGACTGGGAAAAAACCGATGGCCTGATGCCAGCCATCGTGCAACATGCGGTATCCGGC
GAAGTATTGACGCTGGGCTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIRSIDDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWFDDATGKYHVNQYTGDENRTRVTQWETL
DWVQEVQQRGAGEIVLNMMNQDGVRNGYDLTQLKKVRDVCRVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLAGQGVEIRIC

Sequences:

>Translated_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIRSIDDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWFDDATGKYHVNQYTGDENRTRVTQWETL
DWVQEVQQRGAGEIVLNMMNQDGVRNGYDLTQLKKVRDVCRVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLAGQGVEIRIC
>Mature_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIRSIDDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWFDDATGKYHVNQYTGDENRTRVTQWETL
DWVQEVQQRGAGEIVLNMMNQDGVRNGYDLTQLKKVRDVCRVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLAGQGVEIRIC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=94.5736434108527, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI87082028, Length=230, Percent_Identity=26.5217391304348, Blast_Score=66, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=320, Percent_Identity=30, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28369; Mature: 28369

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIRSIDDAAKILSFGADKISINSPALADPTLITRLADRF
ECHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWFDDATGKYHVNQYTGDENRTRVTQWETLDWVQEVQQRGAGEIVLNMMN
CCEEEEEEEECCCCCCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEHHC
QDGVRNGYDLTQLKKVRDVCRVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKAYLAGQGVEIRIC
HHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIRSIDDAAKILSFGADKISINSPALADPTLITRLADRF
ECHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWFDDATGKYHVNQYTGDENRTRVTQWETLDWVQEVQQRGAGEIVLNMMN
CCEEEEEEEECCCCCCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEHHC
QDGVRNGYDLTQLKKVRDVCRVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKAYLAGQGVEIRIC
HHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA