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The map label for this gene is rfbM [H]

Identifier: 29141289

GI number: 29141289

Start: 881855

End: 883294

Strand: Direct

Name: rfbM [H]

Synonym: t0789

Alternate gene names: 29141289

Gene position: 881855-883294 (Clockwise)

Preceding gene: 29141288

Following gene: 29141290

Centisome position: 18.4

GC content: 40.42

Gene sequence:

>1440_bases
ATGTCTTTTCTTCCCGTAATTATGGCTGGCGGCACAGGTAGCCGTTTATGGCCGCTTTCACGCGAATATCATCCGAAGCA
GTTTTTAAGCGTTGAAGGTAAACTATCAATGCTGCAAAATACTATAAAGCGATTAGCTTCACTTTCTACAGAAGAACCCG
TTGTCATTTGCAATGACAGACACCGTTTCTTAGTCGCTGAACAACTCCGTGAAATTGACAAGTTAGCAAATAATATTATT
CTCGAACCAGTAGGCCGTAATACTGCACCAGCGATCGCTCTTGCCGCGTTTTGTGCGCTCCAGAATGCTGATAATGCTGA
TCCTCTTTTGTTGGTTCTTGCTGCAGATCATGTGATCCAGGATGAAATAGCGTTTACGAAAGCTGTCAGACATGCTGAAG
AATACGCCGCAAATGGTAAGCTTGTAACTTTTGGTATTGTTCCAACGCATGCTGAAACGGGTTATGGATATATTCGTCGT
GGTGAGTTGATAGGAAATGACGCTTATGCAGTGGCTGAATTTGTGGAGAAACCGGATATCGATACCGCCAGTGACTATTT
CAAATCAGGGAAATATTACTGGAATAGCGGTATGTTTTTATTTCGTGCAAGCTCTTATTTAAACGAATTGAAGTACTTAT
CACCTGAAATTTACAAAGCTTGTGAAAAGGCGGTAGGACATATAAATCCCGATCTTGATTTTATTCGTATTGATAAAGAA
GAGTTTATGTCATGCCCGAGTGATTCTATCGATTATGCAGTTATGGAGCACACACAGCATGCCGTGGTGATACCAATGAG
TGCTGGCTGGTCGGATGTGGGATCCTGGTCCTCACTTTGGGATATATCGAATAAAGATCATCAGAGAAATGTTTTAAAAG
GAGATATCTTCGCACATGCTTGTAATGATAATTACATTTATTCCGAAGATATGTTTATAAGTGCGATTGGTGTAAGCAAT
CTTGTCATTGTTCAAACAACAGACGCTTTACTGGTGGCTAATAAAGATACAGTACAAGATGTTAAAAAAATTGTCGATTA
TTTAAAACGAAATGATAGGAACGAATATAAACAACATCAAGAAGTTTTCCGCTCTTGGGGAAAATATAATGTGATTGATA
GCGGCAAAAATTACCTCGTCCGATGTATCACTGTTAAGCCGGGTGAGAAATTTGTGGCGCAGATGCATCACCACCGGGCT
GAGCATTGGATAGTATTATCCGGGACTGCTCGTGTTACAAAGGGAGAGCAGATTTATATGGTTTCTGAAAATGAATCAAC
ATTTATTCCTCCGGATACTATTCACGCGCTGGAAAATCCCGGAATGATCCCCCTGCAGTTAATTGAGATTCAATCAGGTA
CCTATCTTGGTGAGGATGATATTATTCGTTTAGAACAACGTTCTGGATTTTCGAAGGAGTGGACTAATGAACGTAGTTAA

Upstream 100 bases:

>100_bases
GCATTGGCAATCTTTACCGTTGTCTACATGTCGCTATTTTAGCATGTACAAGAGTTATTGGAATAATATCCAATATTCTT
CGTCAAAAGAGATAAAATAA

Downstream 100 bases:

>100_bases
TAATAGCCGTGATGTTATTCATTCATCAGGTATTGTGTTTGGAACGAGTGGGGCTCGCGGTCTTGTAAAAGATTTTACAC
CTCAGGTATGTGCTGCTTTT

Product: mannose-1-phosphate guanylyltransferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP; GTP--mannose-1-phosphate guanylyltransferase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSFLPVIMAGGTGSRLWPLSREYHPKQFLSVEGKLSMLQNTIKRLASLSTEEPVVICNDRHRFLVAEQLREIDKLANNII
LEPVGRNTAPAIALAAFCALQNADNADPLLLVLAADHVIQDEIAFTKAVRHAEEYAANGKLVTFGIVPTHAETGYGYIRR
GELIGNDAYAVAEFVEKPDIDTASDYFKSGKYYWNSGMFLFRASSYLNELKYLSPEIYKACEKAVGHINPDLDFIRIDKE
EFMSCPSDSIDYAVMEHTQHAVVIPMSAGWSDVGSWSSLWDISNKDHQRNVLKGDIFAHACNDNYIYSEDMFISAIGVSN
LVIVQTTDALLVANKDTVQDVKKIVDYLKRNDRNEYKQHQEVFRSWGKYNVIDSGKNYLVRCITVKPGEKFVAQMHHHRA
EHWIVLSGTARVTKGEQIYMVSENESTFIPPDTIHALENPGMIPLQLIEIQSGTYLGEDDIIRLEQRSGFSKEWTNERS

Sequences:

>Translated_479_residues
MSFLPVIMAGGTGSRLWPLSREYHPKQFLSVEGKLSMLQNTIKRLASLSTEEPVVICNDRHRFLVAEQLREIDKLANNII
LEPVGRNTAPAIALAAFCALQNADNADPLLLVLAADHVIQDEIAFTKAVRHAEEYAANGKLVTFGIVPTHAETGYGYIRR
GELIGNDAYAVAEFVEKPDIDTASDYFKSGKYYWNSGMFLFRASSYLNELKYLSPEIYKACEKAVGHINPDLDFIRIDKE
EFMSCPSDSIDYAVMEHTQHAVVIPMSAGWSDVGSWSSLWDISNKDHQRNVLKGDIFAHACNDNYIYSEDMFISAIGVSN
LVIVQTTDALLVANKDTVQDVKKIVDYLKRNDRNEYKQHQEVFRSWGKYNVIDSGKNYLVRCITVKPGEKFVAQMHHHRA
EHWIVLSGTARVTKGEQIYMVSENESTFIPPDTIHALENPGMIPLQLIEIQSGTYLGEDDIIRLEQRSGFSKEWTNERS
>Mature_478_residues
SFLPVIMAGGTGSRLWPLSREYHPKQFLSVEGKLSMLQNTIKRLASLSTEEPVVICNDRHRFLVAEQLREIDKLANNIIL
EPVGRNTAPAIALAAFCALQNADNADPLLLVLAADHVIQDEIAFTKAVRHAEEYAANGKLVTFGIVPTHAETGYGYIRRG
ELIGNDAYAVAEFVEKPDIDTASDYFKSGKYYWNSGMFLFRASSYLNELKYLSPEIYKACEKAVGHINPDLDFIRIDKEE
FMSCPSDSIDYAVMEHTQHAVVIPMSAGWSDVGSWSSLWDISNKDHQRNVLKGDIFAHACNDNYIYSEDMFISAIGVSNL
VIVQTTDALLVANKDTVQDVKKIVDYLKRNDRNEYKQHQEVFRSWGKYNVIDSGKNYLVRCITVKPGEKFVAQMHHHRAE
HWIVLSGTARVTKGEQIYMVSENESTFIPPDTIHALENPGMIPLQLIEIQSGTYLGEDDIIRLEQRSGFSKEWTNERS

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group B O antigen [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=471, Percent_Identity=59.2356687898089, Blast_Score=581, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 54092; Mature: 53961

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLPVIMAGGTGSRLWPLSREYHPKQFLSVEGKLSMLQNTIKRLASLSTEEPVVICNDR
CCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCC
HRFLVAEQLREIDKLANNIILEPVGRNTAPAIALAAFCALQNADNADPLLLVLAADHVIQ
CCCHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHH
DEIAFTKAVRHAEEYAANGKLVTFGIVPTHAETGYGYIRRGELIGNDAYAVAEFVEKPDI
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECEEECCCHHHHHHHHHCCCC
DTASDYFKSGKYYWNSGMFLFRASSYLNELKYLSPEIYKACEKAVGHINPDLDFIRIDKE
CCHHHHHHCCCEEEECCEEEEEHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECHH
EFMSCPSDSIDYAVMEHTQHAVVIPMSAGWSDVGSWSSLWDISNKDHQRNVLKGDIFAHA
HHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCHHHHCCCCCCHHCCCCCCCEEEEE
CNDNYIYSEDMFISAIGVSNLVIVQTTDALLVANKDTVQDVKKIVDYLKRNDRNEYKQHQ
CCCCEEECCCCEEEEECCCCEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHH
EVFRSWGKYNVIDSGKNYLVRCITVKPGEKFVAQMHHHRAEHWIVLSGTARVTKGEQIYM
HHHHHCCCEEEEECCCCEEEEEEEECCCHHHHHHHHHCCCCEEEEEECCEEEECCCEEEE
VSENESTFIPPDTIHALENPGMIPLQLIEIQSGTYLGEDDIIRLEQRSGFSKEWTNERS
EECCCCCCCCCHHHHHCCCCCCCEEEEEEEECCCEECCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFLPVIMAGGTGSRLWPLSREYHPKQFLSVEGKLSMLQNTIKRLASLSTEEPVVICNDR
CCCEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCC
HRFLVAEQLREIDKLANNIILEPVGRNTAPAIALAAFCALQNADNADPLLLVLAADHVIQ
CCCHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHH
DEIAFTKAVRHAEEYAANGKLVTFGIVPTHAETGYGYIRRGELIGNDAYAVAEFVEKPDI
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECEEECCCHHHHHHHHHCCCC
DTASDYFKSGKYYWNSGMFLFRASSYLNELKYLSPEIYKACEKAVGHINPDLDFIRIDKE
CCHHHHHHCCCEEEECCEEEEEHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECHH
EFMSCPSDSIDYAVMEHTQHAVVIPMSAGWSDVGSWSSLWDISNKDHQRNVLKGDIFAHA
HHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCHHHHCCCCCCHHCCCCCCCEEEEE
CNDNYIYSEDMFISAIGVSNLVIVQTTDALLVANKDTVQDVKKIVDYLKRNDRNEYKQHQ
CCCCEEECCCCEEEEECCCCEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHH
EVFRSWGKYNVIDSGKNYLVRCITVKPGEKFVAQMHHHRAEHWIVLSGTARVTKGEQIYM
HHHHHCCCEEEEECCCCEEEEEEEECCCHHHHHHHHHCCCCEEEEEECCEEEECCCEEEE
VSENESTFIPPDTIHALENPGMIPLQLIEIQSGTYLGEDDIIRLEQRSGFSKEWTNERS
EECCCCCCCCCHHHHHCCCCCCCEEEEEEEECCCEECCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]