Click here to switch to the map view.

The map label for this gene is alkA [H]

Identifier: 29141250

GI number: 29141250

Start: 833616

End: 834485

Strand: Direct

Name: alkA [H]

Synonym: t0748

Alternate gene names: 29141250

Gene position: 833616-834485 (Clockwise)

Preceding gene: 29141237

Following gene: 29141252

Centisome position: 17.4

GC content: 61.15

Gene sequence:

>870_bases
ATGTTTACCCTGAGCTGGCAGCCGCCTTACGACTGGTCGTGGATGTTGGGCTTTCTTGCCGCGCGTGCGGTGGACGGCGT
GGAAACAGTAGGCGAGGGCTTTTATGCGCGCAGCCTGGTGGTGGGCGAGCATCGCGGACTGGTCAGCGTGAGGCCCCATC
TGCCCACCCACACTGTGCAGGTTAGCGTGAGCGCCGGATTACTTCCCGTCGCGCCGGCGTGTCTGGCGAAAGTTTCGCGT
CTGTTTGATCTTGACTGCCAGCCCGAGCAGGTTGCCGCCGTGCTGGGGCCACTGGGCGAGGACAGACCGGGGCTGCGTTT
ACCGGGATCGGTTGATACGTTTGAACAGGGCGTGCGGGCGATACTGGGGCAACTGGTGAGTGTCGCGATGGCGGCAAGGT
TGACGGCGAAAGTGGCGCGCCGCTATGGCGAGGCGTTGCCGGATGCGCCCGATTATGTCTGTTTTCCCGGTCCGGAAACG
CTGGCGTTGGCCGATCCGCTGGCGCTTAAAGCTCTGGGGATGCCGCTGCGGCGCGCCGAAGCGCTGATTCATCTGGCGCA
GGCGACGCTGGCGGGAAAACTGGCGCTCTCCGCGCCGCCGGATATTGAGCAGAGCGTTAAAAATTTACAAACATTTCCGG
GAATTGGCCGCTGGACGGCGAACTATTTCGCCCTGCGCGGTTGGCAGGCAAAAGATATCTTTTTGCCCGATGATTACTTA
ATTAAGCAGCGCTTCGCCGGGATGACGGCCGCGCAGATCCGTCGCTACGCGGAGCGCTGGAAACCGTGGCGTTCCTACGC
GCTACTGCATATCTGGTATACCCACGGCTGGCAACCGTCAATGGATAGCGAAATAGCTGGTATTCAGTAA

Upstream 100 bases:

>100_bases
GACTTTAGCGAAATGTCGTCGCGGCGACAACTGGAATATGAAAGCAAGGCGTTACGTGCGATTAGCGTTTATGCTGAAAG
GTCAAATAAAGGAGAAAAGT

Downstream 100 bases:

>100_bases
CAAATCCAGCGGTTGCGCTTCGGCGATAGTGTCGCCATAAATCAGATCCACGCCAATACGGGAGAGCGTATCCAGCACCT
GTGGGTTTTGTACCGGCCCG

Product: 3-methyl-adenine DNA glycosylase II

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase II, inducible; TAG II; DNA-3-methyladenine glycosidase II; DNA-3-methyladenine glycosylase II [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MFTLSWQPPYDWSWMLGFLAARAVDGVETVGEGFYARSLVVGEHRGLVSVRPHLPTHTVQVSVSAGLLPVAPACLAKVSR
LFDLDCQPEQVAAVLGPLGEDRPGLRLPGSVDTFEQGVRAILGQLVSVAMAARLTAKVARRYGEALPDAPDYVCFPGPET
LALADPLALKALGMPLRRAEALIHLAQATLAGKLALSAPPDIEQSVKNLQTFPGIGRWTANYFALRGWQAKDIFLPDDYL
IKQRFAGMTAAQIRRYAERWKPWRSYALLHIWYTHGWQPSMDSEIAGIQ

Sequences:

>Translated_289_residues
MFTLSWQPPYDWSWMLGFLAARAVDGVETVGEGFYARSLVVGEHRGLVSVRPHLPTHTVQVSVSAGLLPVAPACLAKVSR
LFDLDCQPEQVAAVLGPLGEDRPGLRLPGSVDTFEQGVRAILGQLVSVAMAARLTAKVARRYGEALPDAPDYVCFPGPET
LALADPLALKALGMPLRRAEALIHLAQATLAGKLALSAPPDIEQSVKNLQTFPGIGRWTANYFALRGWQAKDIFLPDDYL
IKQRFAGMTAAQIRRYAERWKPWRSYALLHIWYTHGWQPSMDSEIAGIQ
>Mature_289_residues
MFTLSWQPPYDWSWMLGFLAARAVDGVETVGEGFYARSLVVGEHRGLVSVRPHLPTHTVQVSVSAGLLPVAPACLAKVSR
LFDLDCQPEQVAAVLGPLGEDRPGLRLPGSVDTFEQGVRAILGQLVSVAMAARLTAKVARRYGEALPDAPDYVCFPGPET
LALADPLALKALGMPLRRAEALIHLAQATLAGKLALSAPPDIEQSVKNLQTFPGIGRWTANYFALRGWQAKDIFLPDDYL
IKQRFAGMTAAQIRRYAERWKPWRSYALLHIWYTHGWQPSMDSEIAGIQ

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=280, Percent_Identity=74.2857142857143, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 31755; Mature: 31755

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00516 ALKYLBASE_DNA_GLYCOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTLSWQPPYDWSWMLGFLAARAVDGVETVGEGFYARSLVVGEHRGLVSVRPHLPTHTVQ
CEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCCEEEECCCCCCEEEE
VSVSAGLLPVAPACLAKVSRLFDLDCQPEQVAAVLGPLGEDRPGLRLPGSVDTFEQGVRA
EEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
ILGQLVSVAMAARLTAKVARRYGEALPDAPDYVCFPGPETLALADPLALKALGMPLRRAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEECCCHHHHHHHCCCHHHHH
ALIHLAQATLAGKLALSAPPDIEQSVKNLQTFPGIGRWTANYFALRGWQAKDIFLPDDYL
HHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCHHHHHHHEEECCCCCEEECCCHHH
IKQRFAGMTAAQIRRYAERWKPWRSYALLHIWYTHGWQPSMDSEIAGIQ
HHHHHCCCCHHHHHHHHHHCCHHHHEEEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MFTLSWQPPYDWSWMLGFLAARAVDGVETVGEGFYARSLVVGEHRGLVSVRPHLPTHTVQ
CEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCCEEEECCCCCCEEEE
VSVSAGLLPVAPACLAKVSRLFDLDCQPEQVAAVLGPLGEDRPGLRLPGSVDTFEQGVRA
EEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
ILGQLVSVAMAARLTAKVARRYGEALPDAPDYVCFPGPETLALADPLALKALGMPLRRAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEECCCHHHHHHHCCCHHHHH
ALIHLAQATLAGKLALSAPPDIEQSVKNLQTFPGIGRWTANYFALRGWQAKDIFLPDDYL
HHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCHHHHHHHEEECCCCCEEECCCHHH
IKQRFAGMTAAQIRRYAERWKPWRSYALLHIWYTHGWQPSMDSEIAGIQ
HHHHHCCCCHHHHHHHHHHCCHHHHEEEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6094528; 9097040; 9278503; 6389535; 3529081; 8706135; 8706136 [H]