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The map label for this gene is yegQ [H]

Identifier: 29141224

GI number: 29141224

Start: 806419

End: 807780

Strand: Reverse

Name: yegQ [H]

Synonym: t0720

Alternate gene names: 29141224

Gene position: 807780-806419 (Counterclockwise)

Preceding gene: 29141238

Following gene: 29141219

Centisome position: 16.86

GC content: 54.77

Gene sequence:

>1362_bases
ATGTTTAAACCAGAACTCCTTTCGCCAGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTACGGTGCCGATGCCGT
CTATGCGGGCCAACCGCGTTACTCTTTACGCGTGCGCAATAACGAATTCAATCACGAAAATTTGCAGCTTGGCATCAACG
AAGCCCACGCGCTCGGAAAAAAATTCTACGTGGTGGTGAACATCGCCCCGCATAACGCCAAGCTCAAAACCTTTATCCGT
GACCTGAAACCCGTCGTCGAGATGGGCCCGGATGCGCTGATCATGTCCGATCCAGGGTTGATTATGCTGGTACGCGAGCA
CTTCCCGGCAATGCCGATTCACCTGTCGGTACAGGCTAACGCCGTAAACTGGGCGACGGTAAAATTCTGGCAGCAGATGG
GGCTGACCCGCGTGATTCTCTCCCGCGAGCTGTCGCTGGAAGAGATTGAGGAAATTCGCCAGCAGGTGCCGGATATGGAA
ATAGAAATTTTCGTCCACGGCGCGCTATGCATGGCCTATTCCGGTCGCTGCCTGCTTTCCGGCTACATCAATAAACGCGA
TCCGAACCAGGGCACCTGCACCAATGCCTGCCGTTGGGAATATAACGTGCAGGAAGGAAAAGAAGACGTTGTCGGCAACA
TCGTGCATAAGCATGAACCGATTCCGGTACAGAACGTTGAGCCGACGCTCGGTATCGGCGCGCCGACGGATAAAGTGTTT
ATGATAGAAGAGGCCCAAAGACCGGGCGAATACATGACCGCGTTCGAAGACGAGCATGGCACCTATATCATGAACTCAAA
AGATTTGCGCGCTATCGCCCACGTGGAGCGCCTGACGAAAATGGGCGTCCACTCGCTGAAAATCGAAGGCCGCACCAAAT
CCTTTTATTACTGCGCCCGTACCGCCCAGGTCTACCGTAAGGCCATCGACGACGCCGCCGCGGGTAAACCCTTCGACCCT
ACGCTGCTGGAAACGCTGGAAGGTCTGGCTCATCGCGGCTATACCGAAGGTTTCCTGCGTCGCCATACGCACGACGATTA
CCAGAATTACGAGTACGGGTACTCCGTTTCCGAACGCCAGCAATTTGTCGGCGAGTTCACCGGCGAGCGTAAAGGCCAAC
TGGCGGCCGTGGCGGTGAAGAATAAATTCTCCGTTGGCGATAGTCTGGAGCTGATGACTCCGCAGGGAAATATCAATTTC
ACTCTGGAACAGATGGAGAACGCCAAAGGCGACGCTATGCCGGTGGCGCCTGGCGATGGCTATACCGTCTGGATGCCCGT
CCCGCAGGACGTTACGCTGGATTACGCACTATTGATGCGTAATTTCTCAGGCGAATCAACGCGTAACCCCCATGCTAAGT
AG

Upstream 100 bases:

>100_bases
CTGTCCGTAAGCGTACAATGCCCGCCCTTAAAGTGAGGACTCTCTTCCAGCCGCCTCATCAGGTGAGGCGGATCTGACCT
GTCATCAGAACGAGAAAATG

Downstream 100 bases:

>100_bases
TTAATTGCGGTTATTTTTTCATGGCGGGAAGATTCTTAGAAATTGATCACATACCGCTTCGCTCAATGCGGGTATTATTC
AACGCGCTGAAAAACATAAC

Product: protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFNHENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIR
DLKPVVEMGPDALIMSDPGLIMLVREHFPAMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRQQVPDME
IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDVVGNIVHKHEPIPVQNVEPTLGIGAPTDKVF
MIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVERLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDP
TLLETLEGLAHRGYTEGFLRRHTHDDYQNYEYGYSVSERQQFVGEFTGERKGQLAAVAVKNKFSVGDSLELMTPQGNINF
TLEQMENAKGDAMPVAPGDGYTVWMPVPQDVTLDYALLMRNFSGESTRNPHAK

Sequences:

>Translated_453_residues
MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFNHENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIR
DLKPVVEMGPDALIMSDPGLIMLVREHFPAMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRQQVPDME
IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDVVGNIVHKHEPIPVQNVEPTLGIGAPTDKVF
MIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVERLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDP
TLLETLEGLAHRGYTEGFLRRHTHDDYQNYEYGYSVSERQQFVGEFTGERKGQLAAVAVKNKFSVGDSLELMTPQGNINF
TLEQMENAKGDAMPVAPGDGYTVWMPVPQDVTLDYALLMRNFSGESTRNPHAK
>Mature_453_residues
MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFNHENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIR
DLKPVVEMGPDALIMSDPGLIMLVREHFPAMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRQQVPDME
IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDVVGNIVHKHEPIPVQNVEPTLGIGAPTDKVF
MIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVERLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDP
TLLETLEGLAHRGYTEGFLRRHTHDDYQNYEYGYSVSERQQFVGEFTGERKGQLAAVAVKNKFSVGDSLELMTPQGNINF
TLEQMENAKGDAMPVAPGDGYTVWMPVPQDVTLDYALLMRNFSGESTRNPHAK

Specific function: Unknown

COG id: COG0826

COG function: function code O; Collagenase and related proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U32 family [H]

Homologues:

Organism=Escherichia coli, GI1788397, Length=453, Percent_Identity=96.2472406181015, Blast_Score=898, Evalue=0.0,
Organism=Escherichia coli, GI87081910, Length=429, Percent_Identity=27.5058275058275, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789548, Length=321, Percent_Identity=28.9719626168224, Blast_Score=129, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001539 [H]

Pfam domain/function: PF01136 Peptidase_U32 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 51085; Mature: 51085

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS01276 PEPTIDASE_U32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFNHENLQLGINEAHALGK
CCCCCCCCCCHHHHCCEEEEEECCCEEEECCCCEEEEEECCCCCCCCEEECCHHHHHCCC
KFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIMLVREHFPAMPIHLSVQAN
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCEEEEEEEC
AVNWATVKFWQQMGLTRVILSRELSLEEIEEIRQQVPDMEIEIFVHGALCMAYSGRCLLS
CCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEEEEEEHCCCCEEEE
GYINKRDPNQGTCTNACRWEYNVQEGKEDVVGNIVHKHEPIPVQNVEPTLGIGAPTDKVF
ECCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
MIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVERLTKMGVHSLKIEGRTKSFYYCAR
EEECCCCCCHHHEEEECCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHH
TAQVYRKAIDDAAAGKPFDPTLLETLEGLAHRGYTEGFLRRHTHDDYQNYEYGYSVSERQ
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHH
QFVGEFTGERKGQLAAVAVKNKFSVGDSLELMTPQGNINFTLEQMENAKGDAMPVAPGDG
HHHHHHCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCEEEHHHHCCCCCCEEECCCCCC
YTVWMPVPQDVTLDYALLMRNFSGESTRNPHAK
EEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFNHENLQLGINEAHALGK
CCCCCCCCCCHHHHCCEEEEEECCCEEEECCCCEEEEEECCCCCCCCEEECCHHHHHCCC
KFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIMLVREHFPAMPIHLSVQAN
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCEEEEEEEC
AVNWATVKFWQQMGLTRVILSRELSLEEIEEIRQQVPDMEIEIFVHGALCMAYSGRCLLS
CCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEEEEEEHCCCCEEEE
GYINKRDPNQGTCTNACRWEYNVQEGKEDVVGNIVHKHEPIPVQNVEPTLGIGAPTDKVF
ECCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
MIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVERLTKMGVHSLKIEGRTKSFYYCAR
EEECCCCCCHHHEEEECCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHH
TAQVYRKAIDDAAAGKPFDPTLLETLEGLAHRGYTEGFLRRHTHDDYQNYEYGYSVSERQ
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHH
QFVGEFTGERKGQLAAVAVKNKFSVGDSLELMTPQGNINFTLEQMENAKGDAMPVAPGDG
HHHHHHCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCEEEHHHHCCCCCCEEECCCCCC
YTVWMPVPQDVTLDYALLMRNFSGESTRNPHAK
EEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]