Click here to switch to the map view.

The map label for this gene is yohI

Identifier: 29141188

GI number: 29141188

Start: 766692

End: 767630

Strand: Direct

Name: yohI

Synonym: t0681

Alternate gene names: 29141188

Gene position: 766692-767630 (Clockwise)

Preceding gene: 29141187

Following gene: 29141190

Centisome position: 16.0

GC content: 57.4

Gene sequence:

>939_bases
ATGCGTGTTTTACTGGCGCCGATGGAAGGCGTGCTCGACGCGTTAGTGCGCGAGCTGCTGACCGAAGTGAATGATTACGA
TCTCTGCATCACCGAATTTGTGCGCGTGGTGGATCAGCTGCTGCCGGTAAAAGTGTTTCATCGCATCTGCCCGGAGTTGC
TTCACGCCAGCCGCACGCCGTCCGGCACGCCGGTGCGTATTCAGCTTCTGGGCCAGCATCCGCAGTGGCTGGCGGAAAAC
GCCGCGCGGGCGACGGCGTTGGGATCGTATGGCGTGGACCTGAACTGCGGCTGTCCGTCAAAAGTGGTGAACGGCAGCGG
CGGCGGCGCGACATTGCTCACAGATCCCGAACTCATCTATCAGGGCGCGAAAGCGATGCGGGCCGCGGTACCGTCGCATC
TGCCGGTGACGGTAAAAGTGCGTCTCGGCTGGGATAGCGGCGATAGAAAATTTGAAATCGCCGATGCGGTGCAGCAGGCC
GGCGCCAGTGAACTGGTGGTGCATGGCCGTACCAAAGCGCAGGGCTACCGCGCCGAGCATATCGACTGGCAGGCGATCGG
CGAAATACGCCAGCGTCTGACTATTCCGGTTATCGCTAATGGCGAAATCTGGGACTGGCAGAGCGCGCAGGCATGTATGG
CGACCAGCGGCTGCGATGCGGTGATGATTGGCCGCGGGGCGTTAAATATTCCTAACCTGAGCCGGGTGGTGAAGTATAAC
GAACCGCGTATGCCGTGGCCGGAAGTGGTAACGTTATTACAAAAATATACCCGACTGGAAAAGCAGGGCGATACCGGTTT
ATACCATGTCGCGCGTATTAAACAGTGGTTGGGATATTTACGTAAGGAATATATTGAGGCGACAGAACTCTTTCAGTCGA
TTCGGGCGTTAAACCGTTCGTCCGAGATTGCGCGGGTGATTCAGGCTATTAAAATCTAA

Upstream 100 bases:

>100_bases
CGTGACGTAGCGTGGCGTTTGCGCCGCCACCCGGCAAAAAGTTTTGCTCATCGCAGAGAGGCGTTATGATAGCGCCTCTT
TTTTTGCTGTGGATACCGAT

Downstream 100 bases:

>100_bases
TCTCTACAGCGTCCAGAACGTGTGCCGGGCAGATACTATCGCTGCCCGGCGCAGGAATCAGAAAATCATCTTAAACACGC
CCGTTACCACCAACAGCCCA

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELLHASRTPSGTPVRIQLLGQHPQWLAEN
AARATALGSYGVDLNCGCPSKVVNGSGGGATLLTDPELIYQGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQA
GASELVVHGRTKAQGYRAEHIDWQAIGEIRQRLTIPVIANGEIWDWQSAQACMATSGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVTLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRSSEIARVIQAIKI

Sequences:

>Translated_312_residues
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELLHASRTPSGTPVRIQLLGQHPQWLAEN
AARATALGSYGVDLNCGCPSKVVNGSGGGATLLTDPELIYQGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQA
GASELVVHGRTKAQGYRAEHIDWQAIGEIRQRLTIPVIANGEIWDWQSAQACMATSGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVTLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRSSEIARVIQAIKI
>Mature_312_residues
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELLHASRTPSGTPVRIQLLGQHPQWLAEN
AARATALGSYGVDLNCGCPSKVVNGSGGGATLLTDPELIYQGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQA
GASELVVHGRTKAQGYRAEHIDWQAIGEIRQRLTIPVIANGEIWDWQSAQACMATSGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVTLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRSSEIARVIQAIKI

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily

Homologues:

Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=29.004329004329, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI40807366, Length=236, Percent_Identity=29.6610169491525, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI8923374, Length=269, Percent_Identity=27.1375464684015, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI239788483, Length=177, Percent_Identity=31.0734463276836, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI239788462, Length=177, Percent_Identity=31.0734463276836, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788462, Length=312, Percent_Identity=89.4230769230769, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1789660, Length=322, Percent_Identity=29.1925465838509, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI145693211, Length=249, Percent_Identity=27.710843373494, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=33.4883720930233, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17543114, Length=240, Percent_Identity=25.8333333333333, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=144, Percent_Identity=34.0277777777778, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=202, Percent_Identity=28.7128712871287, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19921524, Length=145, Percent_Identity=37.2413793103448, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585320, Length=209, Percent_Identity=32.0574162679426, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24580595, Length=179, Percent_Identity=30.1675977653631, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19920448, Length=179, Percent_Identity=30.1675977653631, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSC_SALTI (Q8Z5B2)

Other databases:

- EMBL:   AL627273
- EMBL:   AE014613
- RefSeq:   NP_456733.1
- RefSeq:   NP_804530.1
- ProteinModelPortal:   Q8Z5B2
- SMR:   Q8Z5B2
- GeneID:   1069882
- GeneID:   1248736
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t0681
- KEGG:   sty:STY2404
- HOGENOM:   HBG557545
- OMA:   VGGIDWC
- ProtClustDB:   PRK10550
- BioCyc:   SENT209261:T0681-MONOMER
- BioCyc:   SENT220341:STY2404-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 34568; Mature: 34568

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELLHASRTP
CEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SGTPVRIQLLGQHPQWLAENAARATALGSYGVDLNCGCPSKVVNGSGGGATLLTDPELIY
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEECCCCCHHHCCCCCCCEEEEECHHHHH
QGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQAGASELVVHGRTKAQGYRAEH
HHHHHHHHHCCCCCCEEEEEEECCCCCCCEEHHHHHHHHCCCCCEEEECCCCCCCCCCCC
IDWQAIGEIRQRLTIPVIANGEIWDWQSAQACMATSGCDAVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHEECEEEECCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVTLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SEIARVIQAIKI
HHHHHHHHHHCC
>Mature Secondary Structure
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELLHASRTP
CEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SGTPVRIQLLGQHPQWLAENAARATALGSYGVDLNCGCPSKVVNGSGGGATLLTDPELIY
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEECCCCCHHHCCCCCCCEEEEECHHHHH
QGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQAGASELVVHGRTKAQGYRAEH
HHHHHHHHHCCCCCCEEEEEEECCCCCCCEEHHHHHHHHCCCCCEEEECCCCCCCCCCCC
IDWQAIGEIRQRLTIPVIANGEIWDWQSAQACMATSGCDAVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHEECEEEECCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVTLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SEIARVIQAIKI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504