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The map label for this gene is galS [H]

Identifier: 29141173

GI number: 29141173

Start: 749220

End: 750242

Strand: Direct

Name: galS [H]

Synonym: t0664

Alternate gene names: 29141173

Gene position: 749220-750242 (Clockwise)

Preceding gene: 29141172

Following gene: 29141174

Centisome position: 15.63

GC content: 55.62

Gene sequence:

>1023_bases
ATGATCACCATTCGTGATGTAGCGCGCCAGGCTGGCGTGTCTGTAGCGACCGTTTCCCGCGTACTGAATAACAGCGCGTT
GGTCAGTCCCGACACCCGTGACGCCGTTATGCAGGCCGTCACCCTGCTGGGATATCGGCCAAATGCGAATGCGCAAGCGC
TGGCCACTCAGGTGAGCGATACCATCGGCGTCGTAGTCATGGATGTTTCCGATGCCTTTTTCGGCGCTCTGGTGAAAGCC
GTAGATCTGGTCGCGCAGCAGCACCAGAAATATGTTCTCATTGGCAACAGTTATCATGAGGCGGAAAAAGAGCGCCATGC
GATTGAAGTCTTGATCCGTCAGCGTTGTAACGCATTGATTGTTCACTCAAAAGCCTTAACCGATCGCGAGCTGAGCGACT
TTATGGATCAGATCCCCGGTATGGTGCTGATTAACCGTATCGTGCCGGGTTATGCGCATCGTTGTGTCTGTCTCGACAAT
GTGAGCGGCGCCAGAATGGCGACCCGAATGTTGCTGAATAATGGACATCAACGCATCGGCTACCTGGCCTTCAGCCACCG
TATTGAAGATGACGCGATGCGCAGAGAAGGGTGGTTACACGCGCTGCAAGAGCAGGGGGTTGCTGCGTCGGAGAGCTGGA
TTGGCACCGGCACGCCAGATATGCAGGGCGGCGAGTCGGCAATGGTTGAGTTGCTGGGACGCAATCTGCAACTGACGGCG
GTATTTGCCTATAACGATAACATGGCGTCGGGCGCGCTGACGGCGTTAAAAGATAACGGCATCGCCATTCCCTTGCATCT
GTCTGTCATCGGTTTCGATGATATCCCTATTGCTCGTTATACCGATCCTCAGTTGACTACCGTGCGCTATCCTATTGCTT
CTATGGCGAAAATCGCGACCGAGCTGGCGTTACAGGGGGCCGCAGGCACGCTGGATATCACGGCGACGCACTGCTTCATG
CCGACGCTGGTACGGCGCCATTCGGTGGCGTGGCGACAGAATGCGGTACTGATCACTAACTGA

Upstream 100 bases:

>100_bases
TGTGATGTTTCTCACGCAGCCCTGGGCAGGGTGCTGACAAAATAGCCGCCTGCTAACGGGGCATTTGTGAGCATTTTTGA
ACTCAAACAGGGCAGTGAAT

Downstream 100 bases:

>100_bases
TTACGCCCCGCATGTAACCGTTTTCAATCTGTGAGTAAATTCACAGTTTATTAACATTGTGATAGCTATGATGACAACGT
TTGTCGCACTGTAACTAATG

Product: DNA-binding transcriptional regulator GalS

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDN
VSGARMATRMLLNNGHQRIGYLAFSHRIEDDAMRREGWLHALQEQGVAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
VFAYNDNMASGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIATELALQGAAGTLDITATHCFM
PTLVRRHSVAWRQNAVLITN

Sequences:

>Translated_340_residues
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDN
VSGARMATRMLLNNGHQRIGYLAFSHRIEDDAMRREGWLHALQEQGVAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
VFAYNDNMASGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIATELALQGAAGTLDITATHCFM
PTLVRRHSVAWRQNAVLITN
>Mature_340_residues
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDN
VSGARMATRMLLNNGHQRIGYLAFSHRIEDDAMRREGWLHALQEQGVAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
VFAYNDNMASGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIATELALQGAAGTLDITATHCFM
PTLVRRHSVAWRQNAVLITN

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=340, Percent_Identity=87.9411764705882, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1789202, Length=330, Percent_Identity=56.0606060606061, Blast_Score=352, Evalue=1e-98,
Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=36.5269461077844, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1790369, Length=307, Percent_Identity=33.2247557003257, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=31.5315315315315, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1790194, Length=304, Percent_Identity=29.2763157894737, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787580, Length=327, Percent_Identity=28.7461773700306, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI48994940, Length=310, Percent_Identity=26.1290322580645, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1786540, Length=346, Percent_Identity=29.1907514450867, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787906, Length=261, Percent_Identity=29.1187739463602, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=23.7942122186495, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1789456, Length=306, Percent_Identity=27.1241830065359, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790689, Length=291, Percent_Identity=25.7731958762887, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37026; Mature: 37026

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSD
CEEHHHHHHHHCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALI
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEE
VHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDNVSGARMATRMLLNNGHQRIG
EECHHCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHE
YLAFSHRIEDDAMRREGWLHALQEQGVAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
EEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VFAYNDNMASGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIAT
EEEECCCCCCCCEEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHH
ELALQGAAGTLDITATHCFMPTLVRRHSVAWRQNAVLITN
HHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEC
>Mature Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSD
CEEHHHHHHHHCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALI
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEE
VHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDNVSGARMATRMLLNNGHQRIG
EECHHCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHE
YLAFSHRIEDDAMRREGWLHALQEQGVAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
EEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VFAYNDNMASGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIAT
EEEECCCCCCCCEEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHH
ELALQGAAGTLDITATHCFMPTLVRRHSVAWRQNAVLITN
HHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3146019 [H]