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The map label for this gene is gntR [H]

Identifier: 29141034

GI number: 29141034

Start: 591288

End: 592307

Strand: Reverse

Name: gntR [H]

Synonym: t0519

Alternate gene names: 29141034

Gene position: 592307-591288 (Counterclockwise)

Preceding gene: 29141040

Following gene: 29141030

Centisome position: 12.36

GC content: 55.59

Gene sequence:

>1020_bases
ATGTCGATACCCCGTAAACGGCGCAGTACCGGTAAAGTGACTATCGCCGATGTAGCCCAACTTGCCGGTGTAGGCACGAT
GACCGTATCACGAGCCTTACGCACGCCGGAACAGGTCTCTGATAAACTCCGGGAAAAAATCGAAGCGGCAGTGCATGAGC
TGGGCTATATGCCTAATCTCGCCGCCAGCGCGCTGGCGTCTGCCTCCTCGCACACCATCGCGATGGTCGTACCGAACCTT
GCGGAAGCGGGGTGTTCTGAAATGTTCGCCGGGCTGCAACAAATTTTACAGCCCGCAGGCTACCAGATAATGCTGGCGGA
ATCGCAGCATCGCGTGGAACAAGAGGAGAAGCTGCTCGAAACGCTGCTGGCTTCCAACATTGCCGCGGCGATTCTGCTTA
GCGTCGAACATAGCACTACCGTGCGCCAGTGGCTGAAAAATGCCTCTATCCCGGTGATGGAGATGGGCGCGATTCGCAGC
GATCCTATTGATATGAATATCGGTATTGATAACGTTGCAGCAATGTATGAGCTGACGGAAATGCTGATTCAGCGCGGTTA
CCAGAATATCGGGCTGCTATGCGCCAATCAGGAGCAGTGGATTTTTCAGCAACATCTGCACGGCTGGTACAAAGCGATGC
TTCGCCACCATATGTCGCCAAACCGGGTGATTAATGCCGCCTTGCCGCCCAATTTTTCTACCGGCGCGTCGCAACTGCCG
GAGTTTTTACTAGCATGGCCGGAGCTGGACGCGCTGGTATGCGTATCGGATGAGCTGGCCTGCGGCGCGTTATACGAGTG
CCAACGACGGCGGATCAAAGTTCCGGACGATTTAGCGGTGGTCGGGTTTGGCGATAGCGATGTCAGCCGGGTCTGCCAGC
CGCCGTTAACCACCATGGCCGTACCGCACCGTAAGATTGGCAGTGAAGCCGGACGGGCGCTGTTGGAAAGGTTAAATCAG
GGAAACTGGAGCGATCGAAAATCTATCGCCTCCAGTTTATGTATGCGGGAAAGCTGCTAA

Upstream 100 bases:

>100_bases
CAGCAAAAAAGCGGATTTAAGGCGTATACTGCCAGTCATGCGAAGCAAAGAACGACAATAAAAAAGCATCTCAACATGTT
GTAATGACGCAGGAATTTGT

Downstream 100 bases:

>100_bases
AACGCTTATTCCGTCTCTTCCGGTTTCTCCTGAAGCGCGGCTTCGTTTTTGGCGTTGCGGGTCATCCACAGCGCCAGCGC
TTTGAGCGAGTCCGGCGTAA

Product: transcriptional regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSIPRKRRSTGKVTIADVAQLAGVGTMTVSRALRTPEQVSDKLREKIEAAVHELGYMPNLAASALASASSHTIAMVVPNL
AEAGCSEMFAGLQQILQPAGYQIMLAESQHRVEQEEKLLETLLASNIAAAILLSVEHSTTVRQWLKNASIPVMEMGAIRS
DPIDMNIGIDNVAAMYELTEMLIQRGYQNIGLLCANQEQWIFQQHLHGWYKAMLRHHMSPNRVINAALPPNFSTGASQLP
EFLLAWPELDALVCVSDELACGALYECQRRRIKVPDDLAVVGFGDSDVSRVCQPPLTTMAVPHRKIGSEAGRALLERLNQ
GNWSDRKSIASSLCMRESC

Sequences:

>Translated_339_residues
MSIPRKRRSTGKVTIADVAQLAGVGTMTVSRALRTPEQVSDKLREKIEAAVHELGYMPNLAASALASASSHTIAMVVPNL
AEAGCSEMFAGLQQILQPAGYQIMLAESQHRVEQEEKLLETLLASNIAAAILLSVEHSTTVRQWLKNASIPVMEMGAIRS
DPIDMNIGIDNVAAMYELTEMLIQRGYQNIGLLCANQEQWIFQQHLHGWYKAMLRHHMSPNRVINAALPPNFSTGASQLP
EFLLAWPELDALVCVSDELACGALYECQRRRIKVPDDLAVVGFGDSDVSRVCQPPLTTMAVPHRKIGSEAGRALLERLNQ
GNWSDRKSIASSLCMRESC
>Mature_338_residues
SIPRKRRSTGKVTIADVAQLAGVGTMTVSRALRTPEQVSDKLREKIEAAVHELGYMPNLAASALASASSHTIAMVVPNLA
EAGCSEMFAGLQQILQPAGYQIMLAESQHRVEQEEKLLETLLASNIAAAILLSVEHSTTVRQWLKNASIPVMEMGAIRSD
PIDMNIGIDNVAAMYELTEMLIQRGYQNIGLLCANQEQWIFQQHLHGWYKAMLRHHMSPNRVINAALPPNFSTGASQLPE
FLLAWPELDALVCVSDELACGALYECQRRRIKVPDDLAVVGFGDSDVSRVCQPPLTTMAVPHRKIGSEAGRALLERLNQG
NWSDRKSIASSLCMRESC

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=305, Percent_Identity=37.3770491803279, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1790715, Length=313, Percent_Identity=28.4345047923323, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1790369, Length=319, Percent_Identity=28.2131661442006, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1789202, Length=298, Percent_Identity=27.8523489932886, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=27.027027027027, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788474, Length=292, Percent_Identity=30.1369863013699, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=27.1844660194175, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786540, Length=315, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1787580, Length=333, Percent_Identity=25.2252252252252, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787948, Length=311, Percent_Identity=25.4019292604502, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787906, Length=307, Percent_Identity=26.3843648208469, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790689, Length=316, Percent_Identity=26.8987341772152, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786268, Length=321, Percent_Identity=27.4143302180685, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37228; Mature: 37097

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPRKRRSTGKVTIADVAQLAGVGTMTVSRALRTPEQVSDKLREKIEAAVHELGYMPNL
CCCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCH
AASALASASSHTIAMVVPNLAEAGCSEMFAGLQQILQPAGYQIMLAESQHRVEQEEKLLE
HHHHHHCCCCCEEEEEECCHHHCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHH
TLLASNIAAAILLSVEHSTTVRQWLKNASIPVMEMGAIRSDPIDMNIGIDNVAAMYELTE
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHCCCCCCCCEEECCHHHHHHHHHHHH
MLIQRGYQNIGLLCANQEQWIFQQHLHGWYKAMLRHHMSPNRVINAALPPNFSTGASQLP
HHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHH
EFLLAWPELDALVCVSDELACGALYECQRRRIKVPDDLAVVGFGDSDVSRVCQPPLTTMA
HHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCCHHHHC
VPHRKIGSEAGRALLERLNQGNWSDRKSIASSLCMRESC
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIPRKRRSTGKVTIADVAQLAGVGTMTVSRALRTPEQVSDKLREKIEAAVHELGYMPNL
CCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCH
AASALASASSHTIAMVVPNLAEAGCSEMFAGLQQILQPAGYQIMLAESQHRVEQEEKLLE
HHHHHHCCCCCEEEEEECCHHHCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHH
TLLASNIAAAILLSVEHSTTVRQWLKNASIPVMEMGAIRSDPIDMNIGIDNVAAMYELTE
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHCCCCCCCCEEECCHHHHHHHHHHHH
MLIQRGYQNIGLLCANQEQWIFQQHLHGWYKAMLRHHMSPNRVINAALPPNFSTGASQLP
HHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHH
EFLLAWPELDALVCVSDELACGALYECQRRRIKVPDDLAVVGFGDSDVSRVCQPPLTTMA
HHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCCHHHHC
VPHRKIGSEAGRALLERLNQGNWSDRKSIASSLCMRESC
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]