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The map label for this gene is fadI [H]

Identifier: 29140991

GI number: 29140991

Start: 548181

End: 549491

Strand: Direct

Name: fadI [H]

Synonym: t0475

Alternate gene names: 29140991

Gene position: 548181-549491 (Clockwise)

Preceding gene: 29140990

Following gene: 29140992

Centisome position: 11.44

GC content: 59.34

Gene sequence:

>1311_bases
ATGCGTCAGGCTTTACCGTTGGTCACCCGTCAGGGCGATCGCATTGCTATTGTCAGTGGGTTACGAACCCCCTTCGCTCG
TCAGGCCACTGCATTTCATGGTATTCCCGCTGTTGATCTCGGCAAGATGGTGGTAGGCGAGCTGCTGGCGCGCAGTGAAA
TTCCAGCCGATGCCATTGAGCAACTGGTTTTTGGTCAGGTAGTACAAATGCCGGAAGCCCCCAATATTGCGCGCGAAATT
GTGCTGGGTACGGGAATGAATGTTCATACCGATGCCTATAGCGTAAGCCGCGCCTGCGCCACCAGTTTTCAGGCGGTAGC
GAATGTGGCGGAAAGCCTGATGGCCGGCACGATACGCGCGGGTATCGCTGGCGGCGCAGATTCGTCGTCGGTACTGCCAA
TAGGCGTGAGTAAAGCCCTGGCGCGGGTACTGGTTGATGTCAATAAAGCCCGGACGACCCGCCAACGGCTGACGCTTTTT
TCTCGTCTGCGCTTACGCGACCTGCTGCCCGTTCCTCCCGCCGTTGCTGAGTATTCCACCGGTCTGCGCATGGGCGATAC
CGCCGAACAGATGGCGAAAACGTATGGTATTACGCGTGAACAGCAGGACGCGCTGGCGCATCGTTCACATCAGCGCGCCG
CGCAGGCATGGGCGGAGGGTAAACTGGCGGAAGAAGTGATGACGACCTATGTGCCGCCGTATAAAAACCCCTTTGCAGAA
GACAACAACATTCGCGGTGCCTCCACGCTGGCGGACTACGCTAAACTGCGTCCCGCTTTTGACCGAAAACATGGCTCCGT
AACCGCCGCCAACAGTACACCGCTGACCGACGGCGCTGCCGCCGTTATTCTGATGACCGAATCCCGCGCTAAAGAGCTGG
GGCTGCGCCCGCTTGGTTATCTGCGCAGCTACGCCTTTACCGCTATTGATGTCTGGCAGGACATGCTATTGGGGCCGGCC
TGGTCAACGCCGCTGGCGCTGGAGCGGGCCGGGTTGACGATGGCCGATCTGACGCTTTTTGACATGCATGAAGCGTTTGC
CGCTCAGACTCTGGCGAACTTACAACTGCTGGGCAGCGAACGATTTGCCCGTGAGGTGTTTGGTCGTGCGCAGGCGACAG
GGGAGGTGGATGACGCTAAATTCAACGTGCTCGGCGGCTCAATCGCCTACGGGCATCCTTTTGCCGCAACGGGCGCGCGG
ATGATAACCCAAACGTTGCATGAATTACGGCGTCGGGGCGGAGGTTTTGGCCTGGTCACGGCCTGCGCGGCTGGCGGTCT
TGGCGCGGCGATGGTTCTGGAGGCGGAATAA

Upstream 100 bases:

>100_bases
AGCTCCCACTTTCTTTTCTCTCTGCTTGGCTAAATGTAAAAAATTGGTTAAGACTGTGATCAGGTCAGACCACTAATTTA
TTTTTTTACAGGGGAGTACT

Downstream 100 bases:

>100_bases
TGACTACCACATCAGCTTTTATGCTTAGCGTGCGTCTGGATAATGTCGCGGTTGTCGCTATTGATGTCCCTGGAGAAAAG
GTGAACACCCTGAAAGCGGA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MRQALPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPADAIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKALARVLVDVNKARTTRQRLTLF
SRLRLRDLLPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWAEGKLAEEVMTTYVPPYKNPFAE
DNNIRGASTLADYAKLRPAFDRKHGSVTAANSTPLTDGAAAVILMTESRAKELGLRPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALERAGLTMADLTLFDMHEAFAAQTLANLQLLGSERFAREVFGRAQATGEVDDAKFNVLGGSIAYGHPFAATGAR
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE

Sequences:

>Translated_436_residues
MRQALPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPADAIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKALARVLVDVNKARTTRQRLTLF
SRLRLRDLLPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWAEGKLAEEVMTTYVPPYKNPFAE
DNNIRGASTLADYAKLRPAFDRKHGSVTAANSTPLTDGAAAVILMTESRAKELGLRPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALERAGLTMADLTLFDMHEAFAAQTLANLQLLGSERFAREVFGRAQATGEVDDAKFNVLGGSIAYGHPFAATGAR
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE
>Mature_436_residues
MRQALPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPADAIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKALARVLVDVNKARTTRQRLTLF
SRLRLRDLLPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWAEGKLAEEVMTTYVPPYKNPFAE
DNNIRGASTLADYAKLRPAFDRKHGSVTAANSTPLTDGAAAVILMTESRAKELGLRPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALERAGLTMADLTLFDMHEAFAAQTLANLQLLGSERFAREVFGRAQATGEVDDAKFNVLGGSIAYGHPFAATGAR
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=422, Percent_Identity=40.521327014218, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI4557237, Length=424, Percent_Identity=29.2452830188679, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=33.4883720930233, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI167614485, Length=424, Percent_Identity=30.188679245283, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI148539872, Length=427, Percent_Identity=29.5081967213115, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI194353979, Length=258, Percent_Identity=27.906976744186, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1788683, Length=436, Percent_Identity=93.348623853211, Blast_Score=810, Evalue=0.0,
Organism=Escherichia coli, GI1788554, Length=420, Percent_Identity=35.2380952380952, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI48994986, Length=430, Percent_Identity=35.1162790697674, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87082165, Length=422, Percent_Identity=32.2274881516588, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1787663, Length=424, Percent_Identity=33.7264150943396, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=439, Percent_Identity=38.2687927107062, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI133906874, Length=426, Percent_Identity=31.924882629108, Blast_Score=194, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI25147385, Length=420, Percent_Identity=28.0952380952381, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17535921, Length=424, Percent_Identity=28.3018867924528, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=427, Percent_Identity=23.1850117096019, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6325229, Length=436, Percent_Identity=30.5045871559633, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322031, Length=441, Percent_Identity=28.1179138321995, Blast_Score=156, Evalue=7e-39,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=39.7674418604651, Blast_Score=308, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24655093, Length=422, Percent_Identity=30.0947867298578, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24640423, Length=426, Percent_Identity=27.6995305164319, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI17648125, Length=425, Percent_Identity=28.9411764705882, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 46531; Mature: 46531

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQALPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPADAIE
CCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKALARVLVDVNKARTTRQRLTLFSRLRLRDLLPVPPAVAEYST
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWAEGKLAEEVMTTYVPPYKNPFAE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCC
DNNIRGASTLADYAKLRPAFDRKHGSVTAANSTPLTDGAAAVILMTESRAKELGLRPLGY
CCCCCCHHHHHHHHHHCHHHHCCCCCEEECCCCCCCCCCEEEEEEECHHHHHCCCCHHHH
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMADLTLFDMHEAFAAQTLANLQLLGSE
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFAREVFGRAQATGEVDDAKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
HHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMVLEAE
HHHCCCCCEEEEEECC
>Mature Secondary Structure
MRQALPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPADAIE
CCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKALARVLVDVNKARTTRQRLTLFSRLRLRDLLPVPPAVAEYST
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWAEGKLAEEVMTTYVPPYKNPFAE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCC
DNNIRGASTLADYAKLRPAFDRKHGSVTAANSTPLTDGAAAVILMTESRAKELGLRPLGY
CCCCCCHHHHHHHHHHCHHHHCCCCCEEECCCCCCCCCCEEEEEEECHHHHHCCCCHHHH
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMADLTLFDMHEAFAAQTLANLQLLGSE
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFAREVFGRAQATGEVDDAKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
HHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMVLEAE
HHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA