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The map label for this gene is ligA [H]

Identifier: 29140957

GI number: 29140957

Start: 503087

End: 505102

Strand: Direct

Name: ligA [H]

Synonym: t0431

Alternate gene names: 29140957

Gene position: 503087-505102 (Clockwise)

Preceding gene: 29140956

Following gene: 29140958

Centisome position: 10.5

GC content: 57.24

Gene sequence:

>2016_bases
ATGGAACCAATCGAACAACAACTGACAGAACTGCGAACCACGCTTCGCCATCATGAATATCTGTATCATGTGATGGATGC
GCCCGAGATTCTCGACGCCGAATATGACCGCCTGATGCGGGAACTGCGAGAGCTGGAGGCGCAACGTCCGGACCTTATCA
CGCCGGATTCGCCCACGCAGCGCGTTGGCGCGGCGCCGCTGACGGCATTCAACCAGATTCGCCATGAAGTACCGATGCTG
TCGCTCGATAACGTATTTGATGAAGAAAGCTTCCTGGCGTTTAACAAGCGAGTGCAGGATCGTCTAAAAAGCACGGAAAA
TGTCATCTGGTGCTGCGAGCTAAAACTGGATGGCCTGGCGGTCAGCATTCTGTATGAAAACGGCGTGCTGGTGAGCGCTG
CGACGCGTGGCGACGGCACCACCGGTGAAGATATCACTTCAAACGTGCGCACGATTCGCGCCATTCCGTTAAAGCTGCAC
GGCGATAACATTCCGGCGCGTCTTGAAGTTCGCGGAGAGGTATTTTTACCGCAGGCCGGGTTCGAAAAGATCAATGAAGA
CGCGCGCCGTACTGGCGGCAAAGTGTTTGCTAATCCGCGTAACGCGGCGGCGGGCTCGTTGCGTCAGCTCGATCCGCGTA
TTACGGCGAAGCGACCGCTAACATTTTTCTGCTACGGCGTCGGTATTCTGGAAGGCGGAGAGTTGCCGGATACGCATCTG
GGACGGTTGCTGCAATTTAAAGCGTGGGGGTTACCGGTCAGCGATCGCGTGACGCTGTGCGATTCTCCCCAGGCAGTACT
GGATTTTTATCACAACGTCGAAAAAGATCGCCCCACGCTCGGTTTTGATATCGACGGCGTGGTGATTAAGGTCAACTCGC
TGGCTTTGCAGGAACAGCTGGGCTTTGTGGCCCGCGCGCCGCGCTGGGCGGTGGCGTTTAAATTTCCTGCTCAGGAGCAG
ATGACTTTTGTGCGCGACGTTGAGTTTCAGGTCGGGCGCACCGGGGCGATTACGCCGGTGGCGCGTCTAGAACCGGTTCA
GGTGGCTGGTGTGCTGGTCAGCAATGCCACGTTGCATAATGCCGATGAAATTGAGCGGCTTGGCTTGCGCATTGGCGATA
AGGTGGTTATCCGTCGGGCAGGCGATGTGATCCCTCAGGTGGTCAACGTGGTTCTGTCCGAACGCCCTGAAGAGACGCGT
CCGATTGTGTTTCCAACGCACTGTCCGGTGTGCGGATCGGACGTAGAGCGCGTCGAAGGCGAAGCCGTTACCCGTTGTAC
CGGCGGGCTGATTTGCGGCGCGCAGCGTAAAGAGTCGCTGAAACATTTTGTGTCGCGCCGGGCGATGGATGTAGACGGGA
TGGGCGATAAGATCATCGACCAACTGGTGGAAAGGGAGTATGTCCATACGCCAGCGGATCTGTTCCGTCTGACGGCGGGT
AAACTGACCGGCCTGGACCGAATGGGGCCAAAGTCCGCGCAAAATGTTGTTAACGCGCTGGAAAAAGCCAAAGCGACGAC
TTTTGCGCGTTTTCTCTATGCGCTGGGCATCCGTGAAGTGGGTGAAGCGACGGCGGCGGGGCTGGCGGCGTACTTCGGCA
CGCTGGAGGCGCTGCAGGCTGCCACCATTGACGAGTTGCAGAAAGTACCCGACGTGGGGATTGTGGTCGCTACGCACGTC
TTTAACTTTTTTGCCGAAGAGAGCAATCGTGACGTGATTGGGCAGCTACTGGCGGAAGGGGTTCACTGGCCCGCGCCGGT
AGTCATTAATGCGCAAGAGATCGATAGCCCGTTTGCCGGTAAAACCGTGGTACTAACCGGTAGCCTGAGCCAGATGTCCC
GCGATGACGCTAAAGCGCGTTTAGTGGCTCTGGGCGCGAAAGTGGCAGGCAGCGTATCGAAGAAAACCGATCTGGTGATT
GCCGGTGAAGCGGCGGGCTCCAAACTGGCCAAAGCGCAGGAACTGGGTATTACCGTGATTGATGAAGCGGAAATGATCCG
CTTACTGGGTGCCTGA

Upstream 100 bases:

>100_bases
TCCGCGAGGTGATGGACGCTAACGCCTGAGGTTAACGCAGGTGTGACATTTTTAACGTAACCCCCGCCTGTCGGGGGTTT
TTTATCATTGATGGTGTGAT

Downstream 100 bases:

>100_bases
GATGGAAAAAGAGCAGCTGCTTGAAATCGCCAACACCGTGATGCCCTTTGGCAAATATCAGGGACGTCGATTGATCGACC
TTCCGGAAGAGTATTTGCTG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MEPIEQQLTELRTTLRHHEYLYHVMDAPEILDAEYDRLMRELRELEAQRPDLITPDSPTQRVGAAPLTAFNQIRHEVPML
SLDNVFDEESFLAFNKRVQDRLKSTENVIWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
GDNIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGILEGGELPDTHL
GRLLQFKAWGLPVSDRVTLCDSPQAVLDFYHNVEKDRPTLGFDIDGVVIKVNSLALQEQLGFVARAPRWAVAFKFPAQEQ
MTFVRDVEFQVGRTGAITPVARLEPVQVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEETR
PIVFPTHCPVCGSDVERVEGEAVTRCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEREYVHTPADLFRLTAG
KLTGLDRMGPKSAQNVVNALEKAKATTFARFLYALGIREVGEATAAGLAAYFGTLEALQAATIDELQKVPDVGIVVATHV
FNFFAEESNRDVIGQLLAEGVHWPAPVVINAQEIDSPFAGKTVVLTGSLSQMSRDDAKARLVALGAKVAGSVSKKTDLVI
AGEAAGSKLAKAQELGITVIDEAEMIRLLGA

Sequences:

>Translated_671_residues
MEPIEQQLTELRTTLRHHEYLYHVMDAPEILDAEYDRLMRELRELEAQRPDLITPDSPTQRVGAAPLTAFNQIRHEVPML
SLDNVFDEESFLAFNKRVQDRLKSTENVIWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
GDNIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGILEGGELPDTHL
GRLLQFKAWGLPVSDRVTLCDSPQAVLDFYHNVEKDRPTLGFDIDGVVIKVNSLALQEQLGFVARAPRWAVAFKFPAQEQ
MTFVRDVEFQVGRTGAITPVARLEPVQVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEETR
PIVFPTHCPVCGSDVERVEGEAVTRCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEREYVHTPADLFRLTAG
KLTGLDRMGPKSAQNVVNALEKAKATTFARFLYALGIREVGEATAAGLAAYFGTLEALQAATIDELQKVPDVGIVVATHV
FNFFAEESNRDVIGQLLAEGVHWPAPVVINAQEIDSPFAGKTVVLTGSLSQMSRDDAKARLVALGAKVAGSVSKKTDLVI
AGEAAGSKLAKAQELGITVIDEAEMIRLLGA
>Mature_671_residues
MEPIEQQLTELRTTLRHHEYLYHVMDAPEILDAEYDRLMRELRELEAQRPDLITPDSPTQRVGAAPLTAFNQIRHEVPML
SLDNVFDEESFLAFNKRVQDRLKSTENVIWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
GDNIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGILEGGELPDTHL
GRLLQFKAWGLPVSDRVTLCDSPQAVLDFYHNVEKDRPTLGFDIDGVVIKVNSLALQEQLGFVARAPRWAVAFKFPAQEQ
MTFVRDVEFQVGRTGAITPVARLEPVQVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEETR
PIVFPTHCPVCGSDVERVEGEAVTRCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEREYVHTPADLFRLTAG
KLTGLDRMGPKSAQNVVNALEKAKATTFARFLYALGIREVGEATAAGLAAYFGTLEALQAATIDELQKVPDVGIVVATHV
FNFFAEESNRDVIGQLLAEGVHWPAPVVINAQEIDSPFAGKTVVLTGSLSQMSRDDAKARLVALGAKVAGSVSKKTDLVI
AGEAAGSKLAKAQELGITVIDEAEMIRLLGA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=93.1445603576751, Blast_Score=1285, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=587, Percent_Identity=22.9982964224872, Blast_Score=99, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 73410; Mature: 73410

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIEQQLTELRTTLRHHEYLYHVMDAPEILDAEYDRLMRELRELEAQRPDLITPDSPTQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
RVGAAPLTAFNQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKSTENVIWCCELKLDGLA
HCCCCHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEE
VSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGDNIPARLEVRGEVFLPQAG
EEEEEECCEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEECCCCCEEEEECCEEECCCCC
FEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGILEGGELPDTHL
HHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHH
GRLLQFKAWGLPVSDRVTLCDSPQAVLDFYHNVEKDRPTLGFDIDGVVIKVNSLALQEQL
HHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHH
GFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVQVAGVLVSNATLHN
HHHHCCCCEEEEEECCCHHHHHHHHHCHHCCCCCCCCCCHHHCCCHHEEEEEEECCCCCC
ADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEETRPIVFPTHCPVCGSDVERVEG
HHHHHHHHHCCCCHHEEEHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHCC
EAVTRCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEREYVHTPADLFRLTAG
CHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
KLTGLDRMGPKSAQNVVNALEKAKATTFARFLYALGIREVGEATAAGLAAYFGTLEALQA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATIDELQKVPDVGIVVATHVFNFFAEESNRDVIGQLLAEGVHWPAPVVINAQEIDSPFAG
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCC
KTVVLTGSLSQMSRDDAKARLVALGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGITVI
CEEEEECCHHHHHHHHHHHHHHEECHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCEEEE
DEAEMIRLLGA
CHHHHHHHHCC
>Mature Secondary Structure
MEPIEQQLTELRTTLRHHEYLYHVMDAPEILDAEYDRLMRELRELEAQRPDLITPDSPTQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
RVGAAPLTAFNQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKSTENVIWCCELKLDGLA
HCCCCHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEE
VSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGDNIPARLEVRGEVFLPQAG
EEEEEECCEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEECCCCCEEEEECCEEECCCCC
FEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGILEGGELPDTHL
HHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHH
GRLLQFKAWGLPVSDRVTLCDSPQAVLDFYHNVEKDRPTLGFDIDGVVIKVNSLALQEQL
HHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHH
GFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVQVAGVLVSNATLHN
HHHHCCCCEEEEEECCCHHHHHHHHHCHHCCCCCCCCCCHHHCCCHHEEEEEEECCCCCC
ADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEETRPIVFPTHCPVCGSDVERVEG
HHHHHHHHHCCCCHHEEEHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHCC
EAVTRCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEREYVHTPADLFRLTAG
CHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
KLTGLDRMGPKSAQNVVNALEKAKATTFARFLYALGIREVGEATAAGLAAYFGTLEALQA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATIDELQKVPDVGIVVATHVFNFFAEESNRDVIGQLLAEGVHWPAPVVINAQEIDSPFAG
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCC
KTVVLTGSLSQMSRDDAKARLVALGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGITVI
CEEEEECCHHHHHHHHHHHHHHEECHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCEEEE
DEAEMIRLLGA
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA