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The map label for this gene is ptsI [H]

Identifier: 29140952

GI number: 29140952

Start: 497275

End: 499002

Strand: Reverse

Name: ptsI [H]

Synonym: t0425

Alternate gene names: 29140952

Gene position: 499002-497275 (Counterclockwise)

Preceding gene: 29140953

Following gene: 29140951

Centisome position: 10.41

GC content: 51.68

Gene sequence:

>1728_bases
ATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGCAAAGCACTGCTGCTGAAAGAAGACGAAATCGTCATTGA
CCGGAAAAAAATTTCTGCCGACAAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCTGCGCAAC
TGGAAGCGATCAAGACAAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTC
GAAGATGAGGAGCTGGAGCAGGAAATCATAGCCCTGATTAAAGATAAGCACATGACGGCTGACGCAGCCGCACATGAAGT
TATCGAAGGTCAGGCCACTGCCCTGGAAGAACTGGATGACGAATACCTGAAAGAGCGTGCGGCTGACGTACGTGATATCG
GTAAGCGCCTGCTGCGCAACATCCTGGGTCTGGCCATTATCGATCTGAGCGCGATTCAGGAAGAAGTTATCCTGGTTGCC
GCTGACCTGACGCCGTCAGAAACCGCACAGCTGAACCTGCAGAAAGTGCTGGGCTTCATCACCGACGCGGGCGGACGCAC
GTCTCATACCTCCATCATGGCGCGTTCTCTGGAACTGCCAGCGATCGTAGGTACCGGTAGCGTCACCGCTCAGGTGAAAA
ACGGCGACTATCTGATTCTGGATGCCGTAAACAACCAGGTTTACGTCAACCCGACCAACGATGTTATTGAGCAACTGCGC
GCCGTCCAGGAGCAGGTTGCGACCGAGAAAGCGGAACTCGCAAAACTGAAAGATCTGCCGGCAATCACGCTGGATGGACA
TCAGGTTGAAGTTTGCGCCAACATCGGTACCGTTCGTGACGTTGAAGGCGCTGAGCGTAACGGCGCGGAAGGCGTTGGTC
TGTATCGTACTGAATTCCTGTTCATGGATCGCGACGCGCTGCCGACGGAAGAAGAGCAGTTTGCCGCCTATAAAGCGGTC
GCTGAAGCGTGCGGCTCGCAGGCGGTTATCGTCCGTACCATGGACATTGGCGGCGACAAAGAGCTGCCATACATGAACTT
CCCGAAAGAAGAGAACCCGTTCCTGGGCTGGCGCGCCGTGCGTATCGCCATGGATCGCAAAGAGATCCTGCGTGACCAGG
TTCGCGCGATTCTGCGTGCCTCCGCTTTCGGTAAATTGCGCATTATGTTCCCGATGATCATCTCTGTTGAAGAAGTTCGC
GCGCTGCGCAAAGAGATTGAAATCTACAAACAGGAACTGCGTGACGAAGGTAAAGCATTTGACGAAAGCATTGAGATTGG
CGTGATGGTGGAAACACCGGCTGCGGCGACAATTGCGCGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCA
ATGATTTAACGCAGTACACCCTGGCAGTTGACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCC
GTACTGAACTTGATCAAGCAAGTTATTGATGCTTCTCATGCAGAAGGTAAATGGACTGGCATGTGTGGTGAGCTTGCAGG
CGACGAACGTGCTACACTTCTGTTGCTGGGGATGGGTCTGGACGAATTCTCTATGAGCGCCATTTCTATCCCGCGCATTA
AGAAGATTATCCGTAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAG
TTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAA

Upstream 100 bases:

>100_bases
TGAGCAGAAAGCGGTTGAACATCTGGTCAAACTGATGGCGGAACTCGAGTAAGTTTTTTTCCGGGTTCTTTTAAAAATCA
GTCACAAGTAAGGTAGGGTT

Downstream 100 bases:

>100_bases
TCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTAAAATCTCTGGTTTCTGATG
ATAAGAAAGACACCGGAACT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MISGILASPGIAFGKALLLKEDEIVIDRKKISADKVDQEVERFLSGRAKASAQLEAIKTKAGETFGEEKEAIFEGHIMLL
EDEELEQEIIALIKDKHMTADAAAHEVIEGQATALEELDDEYLKERAADVRDIGKRLLRNILGLAIIDLSAIQEEVILVA
ADLTPSETAQLNLQKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTAQVKNGDYLILDAVNNQVYVNPTNDVIEQLR
AVQEQVATEKAELAKLKDLPAITLDGHQVEVCANIGTVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAV
AEACGSQAVIVRTMDIGGDKELPYMNFPKEENPFLGWRAVRIAMDRKEILRDQVRAILRASAFGKLRIMFPMIISVEEVR
ALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFSIGTNDLTQYTLAVDRGNDMISHLYQPMSPS
VLNLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDE
LMTLVNKFIEEKTIC

Sequences:

>Translated_575_residues
MISGILASPGIAFGKALLLKEDEIVIDRKKISADKVDQEVERFLSGRAKASAQLEAIKTKAGETFGEEKEAIFEGHIMLL
EDEELEQEIIALIKDKHMTADAAAHEVIEGQATALEELDDEYLKERAADVRDIGKRLLRNILGLAIIDLSAIQEEVILVA
ADLTPSETAQLNLQKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTAQVKNGDYLILDAVNNQVYVNPTNDVIEQLR
AVQEQVATEKAELAKLKDLPAITLDGHQVEVCANIGTVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAV
AEACGSQAVIVRTMDIGGDKELPYMNFPKEENPFLGWRAVRIAMDRKEILRDQVRAILRASAFGKLRIMFPMIISVEEVR
ALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFSIGTNDLTQYTLAVDRGNDMISHLYQPMSPS
VLNLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDE
LMTLVNKFIEEKTIC
>Mature_575_residues
MISGILASPGIAFGKALLLKEDEIVIDRKKISADKVDQEVERFLSGRAKASAQLEAIKTKAGETFGEEKEAIFEGHIMLL
EDEELEQEIIALIKDKHMTADAAAHEVIEGQATALEELDDEYLKERAADVRDIGKRLLRNILGLAIIDLSAIQEEVILVA
ADLTPSETAQLNLQKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTAQVKNGDYLILDAVNNQVYVNPTNDVIEQLR
AVQEQVATEKAELAKLKDLPAITLDGHQVEVCANIGTVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAV
AEACGSQAVIVRTMDIGGDKELPYMNFPKEENPFLGWRAVRIAMDRKEILRDQVRAILRASAFGKLRIMFPMIISVEEVR
ALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFSIGTNDLTQYTLAVDRGNDMISHLYQPMSPS
VLNLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDE
LMTLVNKFIEEKTIC

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=575, Percent_Identity=97.2173913043478, Blast_Score=1117, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=576, Percent_Identity=38.7152777777778, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI48994992, Length=505, Percent_Identity=38.2178217821782, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1789193, Length=578, Percent_Identity=33.9100346020761, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1787994, Length=449, Percent_Identity=26.2806236080178, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI226510935, Length=151, Percent_Identity=33.7748344370861, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63369; Mature: 63369

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGILASPGIAFGKALLLKEDEIVIDRKKISADKVDQEVERFLSGRAKASAQLEAIKTK
CCCCCCCCCCHHHHHHHEEECCCEEEEHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADAAAHEVIEGQATALEELDD
HCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
EYLKERAADVRDIGKRLLRNILGLAIIDLSAIQEEVILVAADLTPSETAQLNLQKVLGFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHH
TDAGGRTSHTSIMARSLELPAIVGTGSVTAQVKNGDYLILDAVNNQVYVNPTNDVIEQLR
HCCCCCCHHHHHHHHHCCCCEEEECCCEEEEEECCCEEEEEECCCEEEECCHHHHHHHHH
AVQEQVATEKAELAKLKDLPAITLDGHQVEVCANIGTVRDVEGAERNGAEGVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEE
FMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTMDIGGDKELPYMNFPKEENPFLGWRAV
EECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
RIAMDRKEILRDQVRAILRASAFGKLRIMFPMIISVEEVRALRKEIEIYKQELRDEGKAF
EEHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DESIEIGVMVETPAAATIARHLAKEVDFFSIGTNDLTQYTLAVDRGNDMISHLYQPMSPS
CCCEEEEEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEEECCCHHHHHHHCCCCCH
VLNLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNT
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHHCHHHHHHHHHCC
NFEDAKVLAEQALAQPTTDELMTLVNKFIEEKTIC
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISGILASPGIAFGKALLLKEDEIVIDRKKISADKVDQEVERFLSGRAKASAQLEAIKTK
CCCCCCCCCCHHHHHHHEEECCCEEEEHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADAAAHEVIEGQATALEELDD
HCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
EYLKERAADVRDIGKRLLRNILGLAIIDLSAIQEEVILVAADLTPSETAQLNLQKVLGFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHH
TDAGGRTSHTSIMARSLELPAIVGTGSVTAQVKNGDYLILDAVNNQVYVNPTNDVIEQLR
HCCCCCCHHHHHHHHHCCCCEEEECCCEEEEEECCCEEEEEECCCEEEECCHHHHHHHHH
AVQEQVATEKAELAKLKDLPAITLDGHQVEVCANIGTVRDVEGAERNGAEGVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEE
FMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTMDIGGDKELPYMNFPKEENPFLGWRAV
EECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
RIAMDRKEILRDQVRAILRASAFGKLRIMFPMIISVEEVRALRKEIEIYKQELRDEGKAF
EEHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DESIEIGVMVETPAAATIARHLAKEVDFFSIGTNDLTQYTLAVDRGNDMISHLYQPMSPS
CCCEEEEEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEEECCCHHHHHHHCCCCCH
VLNLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNT
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHHCHHHHHHHHHCC
NFEDAKVLAEQALAQPTTDELMTLVNKFIEEKTIC
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2457575; 2960675; 9205837; 9278503; 2411636; 3290198; 9298646; 7876255; 8805571; 9054557; 9541412; 10048929 [H]