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The map label for this gene is yfeG [H]

Identifier: 29140931

GI number: 29140931

Start: 479385

End: 480437

Strand: Direct

Name: yfeG [H]

Synonym: t0404

Alternate gene names: 29140931

Gene position: 479385-480437 (Clockwise)

Preceding gene: 29140930

Following gene: 29140936

Centisome position: 10.0

GC content: 54.51

Gene sequence:

>1053_bases
ATGAAAAAGACCCGTACAGCGAATTTGCACCATCTTTATCATGAAGCGTTACCCGAAGACGTTAAACTTACGCCCAGAGT
CGAGGTGGACAATGTTCATCAGCGACGGACGACGGATGTCTATGAACATGCTCTGACTATCACCGCCTGGCAGCAGATCT
ACGATCAGTTACACCTGGGTAAATTTCATGGCGAGTTCACGGAAATCCTGCTTGATGAGATTCAGGTGTTCCGTGAATAC
ACCGGCCTGGCGCTGCGTCAGTCGTGTCTGGTTTGGCCAAACTCTTTCTGGTTTGGTATTCCGGCGACGCGCGGCGAACA
GGGATTTATTGGCGCGCAGGGGCTCGGCAGCGCCGAGATTGCCACCCGACCGGGAGGCACCGAGTTTGAACTGAGCACGC
CGGATGATTACACCATTCTGGGCGTCGTTATCTCGGAAGATGTTATTTCCCGTCAGGCCACGTTTTTGCATAACCCGGAA
AGGGTGCTGCATATGCTGCGTAACCAGTTAGCGCTGGAGGTAAAAGAGCAGCATAAAGCGGCGCTGTGGGGCTTTGTGCA
GCAGGCGCTGGCCACCTTTAGCGAGAGTCCTGAAACCCTGCATCAACCTGCGGTGCGTAAGGTCTTGAGTGATAATTTGT
TGCTGGCGATGGGCACGATGCTGGAAGAGGCGAAGCCGATTCATAGCGCCGAAAGCATCAGCCATCAGGGATATCGTAGG
CTACTGTCGCGGGCGCGGGAATATGTGCTGGAAAATATGTCGGAGCCGCTGACGGTGCTCGACTTATGTAACCAGCTACA
CGTAAGCCGTCGCACGCTGCAAAATGCGTTTCACGCCATTTTGGGCATTGGCCCCAATGCGTGGCTGAAACGTATTCGCC
TCAATGCAGTGCGCCGGGAGTTGATTAGCCCGTGGTCGCAGAGCACCACGGTCAGAGATGCCGCGATGCAGTGGGGATTC
TGGCATCTGGGGCAGTTTGCCACCGATTATCAGCAATTATTTGCCGAAAAACCGTCGTTGACGCTGCATCAACGGATGCG
GCAATGGGCTTGA

Upstream 100 bases:

>100_bases
TTTCTGACGGAGCGTTGCGTAAACGCAGTAGTCGCTATCGCATCAAAAATTAATCTGTCGGAGGGCCGGGCGTCCTGGCG
GGCGTCCGGCATAGAAGATC

Downstream 100 bases:

>100_bases
TATTGATGGGTAGGCTCTCCGGTTGCTGATAACGTGCGCGTGTCGGGAATTGCCCGGTGGCGGCTGGCGCCTTACCGGGC
CTACGGATTCGCTGCTGTTT

Product: transcriptional regulator EutR

Products: NA

Alternate protein names: Ethanolamine operon regulatory protein [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKKTRTANLHHLYHEALPEDVKLTPRVEVDNVHQRRTTDVYEHALTITAWQQIYDQLHLGKFHGEFTEILLDEIQVFREY
TGLALRQSCLVWPNSFWFGIPATRGEQGFIGAQGLGSAEIATRPGGTEFELSTPDDYTILGVVISEDVISRQATFLHNPE
RVLHMLRNQLALEVKEQHKAALWGFVQQALATFSESPETLHQPAVRKVLSDNLLLAMGTMLEEAKPIHSAESISHQGYRR
LLSRAREYVLENMSEPLTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVRDAAMQWGF
WHLGQFATDYQQLFAEKPSLTLHQRMRQWA

Sequences:

>Translated_350_residues
MKKTRTANLHHLYHEALPEDVKLTPRVEVDNVHQRRTTDVYEHALTITAWQQIYDQLHLGKFHGEFTEILLDEIQVFREY
TGLALRQSCLVWPNSFWFGIPATRGEQGFIGAQGLGSAEIATRPGGTEFELSTPDDYTILGVVISEDVISRQATFLHNPE
RVLHMLRNQLALEVKEQHKAALWGFVQQALATFSESPETLHQPAVRKVLSDNLLLAMGTMLEEAKPIHSAESISHQGYRR
LLSRAREYVLENMSEPLTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVRDAAMQWGF
WHLGQFATDYQQLFAEKPSLTLHQRMRQWA
>Mature_350_residues
MKKTRTANLHHLYHEALPEDVKLTPRVEVDNVHQRRTTDVYEHALTITAWQQIYDQLHLGKFHGEFTEILLDEIQVFREY
TGLALRQSCLVWPNSFWFGIPATRGEQGFIGAQGLGSAEIATRPGGTEFELSTPDDYTILGVVISEDVISRQATFLHNPE
RVLHMLRNQLALEVKEQHKAALWGFVQQALATFSESPETLHQPAVRKVLSDNLLLAMGTMLEEAKPIHSAESISHQGYRR
LLSRAREYVLENMSEPLTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVRDAAMQWGF
WHLGQFATDYQQLFAEKPSLTLHQRMRQWA

Specific function: Activates the transcription of the eut operon. Also positively regulates its own transcription. Probably binds ethanolamine and vitamin B12 as effectors [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=350, Percent_Identity=91.1428571428572, Blast_Score=673, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 40129; Mature: 40129

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTRTANLHHLYHEALPEDVKLTPRVEVDNVHQRRTTDVYEHALTITAWQQIYDQLHLG
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFHGEFTEILLDEIQVFREYTGLALRQSCLVWPNSFWFGIPATRGEQGFIGAQGLGSAEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCE
ATRPGGTEFELSTPDDYTILGVVISEDVISRQATFLHNPERVLHMLRNQLALEVKEQHKA
EECCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ALWGFVQQALATFSESPETLHQPAVRKVLSDNLLLAMGTMLEEAKPIHSAESISHQGYRR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLSRAREYVLENMSEPLTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LISPWSQSTTVRDAAMQWGFWHLGQFATDYQQLFAEKPSLTLHQRMRQWA
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKTRTANLHHLYHEALPEDVKLTPRVEVDNVHQRRTTDVYEHALTITAWQQIYDQLHLG
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFHGEFTEILLDEIQVFREYTGLALRQSCLVWPNSFWFGIPATRGEQGFIGAQGLGSAEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCE
ATRPGGTEFELSTPDDYTILGVVISEDVISRQATFLHNPERVLHMLRNQLALEVKEQHKA
EECCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ALWGFVQQALATFSESPETLHQPAVRKVLSDNLLLAMGTMLEEAKPIHSAESISHQGYRR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLSRAREYVLENMSEPLTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LISPWSQSTTVRDAAMQWGFWHLGQFATDYQQLFAEKPSLTLHQRMRQWA
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503 [H]