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The map label for this gene is eutB [H]

Identifier: 29140927

GI number: 29140927

Start: 475885

End: 477246

Strand: Direct

Name: eutB [H]

Synonym: t0400

Alternate gene names: 29140927

Gene position: 475885-477246 (Clockwise)

Preceding gene: 29140926

Following gene: 29140928

Centisome position: 9.93

GC content: 56.53

Gene sequence:

>1362_bases
ATGAAACTAAAGACCACATTGTTCGGCAATGTTTATCAGTTTAAGGATGTAAAAGAGGTACTGGCTAAAGCCAACGAACT
GCGTTCGGGGGATGTGCTGGCCGGGGTTGCCGCGGCAAGTTCGCAGGAGCGCGTAGCGGCAAAACAGGTACTGTCGGAAA
TGACGGTGGCGGATATCCGCAACAATCCGGTGATTGCCTATGAAGAGGACTGCGTGACGCGCCTGATTCAGGATGACGTC
AACGAAACGGCCTATAATCGGATTAAAAACTGGAGCATCAGCGAACTGCGCGAATACGTACTGAGCGATGAAACCTCCGT
GGACGACATCGCGTTTACCCGCAAAGGCCTGACCTCCGAAGTGGTGGCGGCAGTGGCGAAAATCTGCTCCAACGCCGACC
TGATCTACGGCGGTAAGAAAATGCCGGTGATCAAAAAAGCCAATACCACCATCGGTATTCCGGGCACCTTTAGCTGCCGT
TTGCAGCCGAACGATACCCGTGACGATGTGCAGAGTATCGCCGCGCAAATCTACGAAGGGCTTTCTTTCGGCGCAGGCGA
TGCGGTGATCGGCGTTAACCCGGTGACCGATGACGTGGAGAACCTGACCCGCGTGCTCGACACCGTTTACGGCGTTATCG
ATAAATTCAATATTCCGACCCAGGGCTGCGTGCTGGCGCACGTCACCACCCAGATTGAAGCGATTCGTCGCGGCGCGCCA
GGCGGACTGATTTTCCAGAGCATTTGCGGCAGCGAGAAGGGCTTAAAAGAGTTCGGTGTCGAGCTGGCAATGCTCGACGA
AGCGCGGGCAGTGGGGGCGGAGTTCAACCGCATCGCCGGGGAAAACTGCCTGTACTTTGAAACCGGACAGGGCTCGGCGC
TGTCGGCTGGTGCGAACTTTGGTGCCGACCAGGTGACGATGGAAGCGCGTAACTACGGGCTGGCTCGCTACTACGATCCG
TTCCTGGTGAACACCGTGGTGGGCTTTATCGGGCCGGAGTATCTCTACAACGACAGGCAGATTATCCGCGCCGGTCTCGA
AGATCACTTTATGGGCAAGCTGAGCGGCATCTCGATGGGCTGCGACTGCTGCTATACCAACCATGCCGACGCCGACCAGA
ACCTTAACGAAAACCTGATGATTCTGCTCGCCACTGCCGGCTGTAACTACATCATGGGGATGCCGCTCGGCGACGACATC
ATGCTCAACTACCAGACTACCGCTTTCCACGATACCGCCACCGTCCGTCAGTTGCTGAATTTACGGCCGTCGCCGGAGTT
TGAACGCTGGCTGGAAACGATGGGCATTATGGCAAACGGTCGTCTGACCAAACGGGCGGGCGATCCGTCACTGTTCTTCT
GA

Upstream 100 bases:

>100_bases
GGGCGGGAGACTATATCGACATTGGTACGCCTCTTTTTGGCGGATCGGTTGTGCCGGTGACGGTGAAATCACTCGCATTT
CCTTCCTGAGGGAACGACTT

Downstream 100 bases:

>100_bases
TGACGCGGGGATAACACCATGGATCAAAAACAGATTGAAGAAATTGTACGTAGCGTGATGGCGTCAATGGGACAGGACGT
ACCGCAGCCCGTCGCGCCGT

Product: ethanolamine ammonia-lyase heavy chain

Products: NA

Alternate protein names: Ethanolamine ammonia-lyase large subunit [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIRNNPVIAYEEDCVTRLIQDDV
NETAYNRIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGGKKMPVIKKANTTIGIPGTFSCR
LQPNDTRDDVQSIAAQIYEGLSFGAGDAVIGVNPVTDDVENLTRVLDTVYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARYYDP
FLVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDI
MLNYQTTAFHDTATVRQLLNLRPSPEFERWLETMGIMANGRLTKRAGDPSLFF

Sequences:

>Translated_453_residues
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIRNNPVIAYEEDCVTRLIQDDV
NETAYNRIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGGKKMPVIKKANTTIGIPGTFSCR
LQPNDTRDDVQSIAAQIYEGLSFGAGDAVIGVNPVTDDVENLTRVLDTVYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARYYDP
FLVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDI
MLNYQTTAFHDTATVRQLLNLRPSPEFERWLETMGIMANGRLTKRAGDPSLFF
>Mature_453_residues
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIRNNPVIAYEEDCVTRLIQDDV
NETAYNRIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGGKKMPVIKKANTTIGIPGTFSCR
LQPNDTRDDVQSIAAQIYEGLSFGAGDAVIGVNPVTDDVENLTRVLDTVYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARYYDP
FLVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDI
MLNYQTTAFHDTATVRQLLNLRPSPEFERWLETMGIMANGRLTKRAGDPSLFF

Specific function: Ethanolamine utilization. [C]

COG id: COG4303

COG function: function code E; Ethanolamine ammonia-lyase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082106, Length=453, Percent_Identity=97.7924944812362, Blast_Score=922, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010628 [H]

Pfam domain/function: PF06751 EutB [H]

EC number: =4.3.1.7 [H]

Molecular weight: Translated: 49476; Mature: 49476

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIR
CCEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHC
NNPVIAYEEDCVTRLIQDDVNETAYNRIKNWSISELREYVLSDETSVDDIAFTRKGLTSE
CCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHCCCHHH
VVAAVAKICSNADLIYGGKKMPVIKKANTTIGIPGTFSCRLQPNDTRDDVQSIAAQIYEG
HHHHHHHHHCCCCEEECCCCCCEEEECCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHC
LSFGAGDAVIGVNPVTDDVENLTRVLDTVYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCC
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANF
CCHHHHHHCCCCCCHHHHCCEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCC
GADQVTMEARNYGLARYYDPFLVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMG
CCCCEEHHHHHCCCHHHCCHHHHHHHHHHHCHHHHHCCHHHHHHCHHHHHHHHHCCCCCC
CDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLN
CCEEECCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCEEEEEEECCCCCHHHHHHHHC
LRPSPEFERWLETMGIMANGRLTKRAGDPSLFF
CCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIR
CCEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHC
NNPVIAYEEDCVTRLIQDDVNETAYNRIKNWSISELREYVLSDETSVDDIAFTRKGLTSE
CCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHCCCHHH
VVAAVAKICSNADLIYGGKKMPVIKKANTTIGIPGTFSCRLQPNDTRDDVQSIAAQIYEG
HHHHHHHHHCCCCEEECCCCCCEEEECCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHC
LSFGAGDAVIGVNPVTDDVENLTRVLDTVYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCC
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANF
CCHHHHHHCCCCCCHHHHCCEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCC
GADQVTMEARNYGLARYYDPFLVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMG
CCCCEEHHHHHCCCHHHCCHHHHHHHHHHHCHHHHHCCHHHHHHCHHHHHHHHHCCCCCC
CDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLN
CCEEECCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCEEEEEEECCCCCHHHHHHHHC
LRPSPEFERWLETMGIMANGRLTKRAGDPSLFF
CCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]