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The map label for this gene is dapE [H]

Identifier: 29140903

GI number: 29140903

Start: 445715

End: 446842

Strand: Reverse

Name: dapE [H]

Synonym: t0376

Alternate gene names: 29140903

Gene position: 446842-445715 (Counterclockwise)

Preceding gene: 29140904

Following gene: 29140902

Centisome position: 9.32

GC content: 54.34

Gene sequence:

>1128_bases
ATGTCGTGCCCGGTTATTGAGCTGACACAGCAACTTATTCGTCGCCCCTCCCTGAGTCCGGATGACGTAGGCTGTCAGGC
ATTAATGATTGAACGCCTGCGTAAAATCGGTTTTACCATTGAGCATATGGATTTTGGCGACACGCAAAATTTTTGGGCAT
GGCGTGGGCGTGGCGAAACGCTGGCGTTCGCCGGGCATACTGACGTCGTACCGGCTGGCGATGTCGATCGCTGGATCAAT
CCGCCGTTTGAACCTACGATTCGCGACGGAATGTTGTTCGGTCGCGGCGCGGCGGATATGAAAGGTTCACTGGCGGCCAT
GGTGGTGGCTGCTGAACGTTTTGTTGCACAGCATCCTCATCACCGGGGCCGCCTCGCATTTTTGATTACCTCTGATGAAG
AAGCCAGCGCAAAAAATGGCACCGTAAAGGTGGTTGAAGCGCTGATGGCCCGTAACGAGCGGCTGGATTACTGTCTGGTC
GGCGAACCTTCCAGTACTGAAATTGTGGGCGATGTCGTCAAAAACGGGCGTCGCGGTTCCCTCACCTGTAACCTCACTAT
TCATGGCGTTCAGGGGCACGTTGCCTATCCGCATCTGGCGGATAACCCTGTTCATCGCGCCGCGCCTTTTCTCAATGAAC
TGGTCGCTATCGAGTGGGATCGGGGCAATGACTTTTTCCCGGCCACCAGTATGCAGATAGCCAATATCCAGGCAGGCACC
GGCAGCAATAATGTCATTCCCGGCGAACTGTTCGTGCAGTTTAACTTCCGTTTTAGCACGGAGCTGACCGATGAGATGAT
CAAAGAACGGGTACATGCGCTGCTTGAGAAGCATCAGCTACGCTATACCGTAGACTGGTGGCTTTCCGGTCAGCCGTTCC
TGACCGCACGCGGAAAGCTGGTGGACGCGGTGGTAAACGCCATTGAGCACTATAATGAAATTAAACCGCAATTACTGACG
ACGGGCGGTACGTCCGACGGACGTTTTATCGCCCGTATGGGAGCGCAGGTGGTAGAACTGGGGCCGGTGAACGCCACCAT
TCATAAAATTAACGAATGTGTGAATGCCGCCGATCTCCAGCTACTGGCCCGTATGTATCAACGTATTATGGAACAGCTCG
TCGCCTGA

Upstream 100 bases:

>100_bases
GGCAATCATCAAACGCCCATTGCTCTGCGCGCCCGGGAAGCCTATGCTGCTTGGTTTCAGTGAATCCCGTTATCAGCAGT
TTTTTGATGAGGTGTAGTCT

Downstream 100 bases:

>100_bases
TGAGCGTTCCGGTAAGAGGAAATAAGCATGGACTGGCTGGCAAAATATTGGTGGATTCTGGTATTGGTGTTTCTGGTAGG
CGTACTGCTGAATGTGATCA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSCPVIELTQQLIRRPSLSPDDVGCQALMIERLRKIGFTIEHMDFGDTQNFWAWRGRGETLAFAGHTDVVPAGDVDRWIN
PPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPHHRGRLAFLITSDEEASAKNGTVKVVEALMARNERLDYCLV
GEPSSTEIVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDRGNDFFPATSMQIANIQAGT
GSNNVIPGELFVQFNFRFSTELTDEMIKERVHALLEKHQLRYTVDWWLSGQPFLTARGKLVDAVVNAIEHYNEIKPQLLT
TGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA

Sequences:

>Translated_375_residues
MSCPVIELTQQLIRRPSLSPDDVGCQALMIERLRKIGFTIEHMDFGDTQNFWAWRGRGETLAFAGHTDVVPAGDVDRWIN
PPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPHHRGRLAFLITSDEEASAKNGTVKVVEALMARNERLDYCLV
GEPSSTEIVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDRGNDFFPATSMQIANIQAGT
GSNNVIPGELFVQFNFRFSTELTDEMIKERVHALLEKHQLRYTVDWWLSGQPFLTARGKLVDAVVNAIEHYNEIKPQLLT
TGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA
>Mature_374_residues
SCPVIELTQQLIRRPSLSPDDVGCQALMIERLRKIGFTIEHMDFGDTQNFWAWRGRGETLAFAGHTDVVPAGDVDRWINP
PFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPHHRGRLAFLITSDEEASAKNGTVKVVEALMARNERLDYCLVG
EPSSTEIVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDRGNDFFPATSMQIANIQAGTG
SNNVIPGELFVQFNFRFSTELTDEMIKERVHALLEKHQLRYTVDWWLSGQPFLTARGKLVDAVVNAIEHYNEIKPQLLTT
GGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=94.4, Blast_Score=740, Evalue=0.0,
Organism=Escherichia coli, GI1790395, Length=259, Percent_Identity=27.4131274131274, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789236, Length=385, Percent_Identity=23.3766233766234, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41621; Mature: 41490

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCPVIELTQQLIRRPSLSPDDVGCQALMIERLRKIGFTIEHMDFGDTQNFWAWRGRGET
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCEEECCCCCE
LAFAGHTDVVPAGDVDRWINPPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPH
EEEECCCCCCCCCCCHHHCCCCCCCCHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
HRGRLAFLITSDEEASAKNGTVKVVEALMARNERLDYCLVGEPSSTEIVGDVVKNGRRGS
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCCE
LTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDRGNDFFPATSMQIANIQAGT
EEEEEEEEECCCCEECCCCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCCEEEEEEECCC
GSNNVIPGELFVQFNFRFSTELTDEMIKERVHALLEKHQLRYTVDWWLSGQPFLTARGKL
CCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEECHHH
VDAVVNAIEHYNEIKPQLLTTGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQ
HHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCCEEEECCCHHHHHHHHHHHCHHHHH
LLARMYQRIMEQLVA
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SCPVIELTQQLIRRPSLSPDDVGCQALMIERLRKIGFTIEHMDFGDTQNFWAWRGRGET
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCEEECCCCCE
LAFAGHTDVVPAGDVDRWINPPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPH
EEEECCCCCCCCCCCHHHCCCCCCCCHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
HRGRLAFLITSDEEASAKNGTVKVVEALMARNERLDYCLVGEPSSTEIVGDVVKNGRRGS
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCCE
LTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDRGNDFFPATSMQIANIQAGT
EEEEEEEEECCCCEECCCCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCCEEEEEEECCC
GSNNVIPGELFVQFNFRFSTELTDEMIKERVHALLEKHQLRYTVDWWLSGQPFLTARGKL
CCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEECHHH
VDAVVNAIEHYNEIKPQLLTTGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQ
HHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCCEEEECCCHHHHHHHHHHHCHHHHH
LLARMYQRIMEQLVA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA