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The map label for this gene is purN [H]

Identifier: 29140885

GI number: 29140885

Start: 426386

End: 427024

Strand: Reverse

Name: purN [H]

Synonym: t0357

Alternate gene names: 29140885

Gene position: 427024-426386 (Counterclockwise)

Preceding gene: 29140886

Following gene: 29140884

Centisome position: 8.91

GC content: 56.34

Gene sequence:

>639_bases
ATGAATATTGTGGTGCTGATTTCCGGCAATGGAAGCAATTTACAGGCGATTATCGATGCCTGCGAAGCGAAGAAAATTAA
AGGCACCCTCAGGGCAGTATTCAGCAATAAGGCCGACGCGTTCGGCCTTGAACGCGCCAGAGAAGCCGGCATTCCGGCGC
AAGCGCTGACTGCCGATCGGTTTGACAGCCGCGACGCCTTCGATCGCGAGTTGATTCGCAAGATAGACGCGTATGCGCCC
GATGTGGTCGTGCTGGCCGGTTTTATGCGTATTCTGAGTCCGATGTTTGTCGCGCATTACTACGGGCGTCTGCTGAACAT
TCACCCTTCCCTGCTGCCAAAATATCCGGGGTTGCATACCCATCGCCAGGCGCTGGAAAACGGCGATGAGGAGCACGGTA
CCTCGGTGCATTTCGTGACAGACGAACTCGACGGCGGCCCGGTCATTCTCCAGGCGAAGGTGCCGGTTTTTGCCAACGAC
AGCGAAGATGATATCACCGCACGCGTACAAACTCAGGAACATGCGATTTATCCGCTGGTGATTGGCTGGTTTGCGCAGGG
GCGTCTAAAGATGCGCGATAACGCCGCCTGGCTGGACGGGCGTCGTCTGCCGCCGCAAGGTTATGCGTCTGACGAATAA

Upstream 100 bases:

>100_bases
CGGACAAAGCGCTTGCTCTGCTAAACGAGAAAGGTGAAAACGCATGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAA
CAGCGTGTGGTTATTGAATA

Downstream 100 bases:

>100_bases
TATGTCATCGGCCAGGGCTGCGTCTGCTTGCCGGATGGCGGCATAAATGCCTATCCGGCCTACTCTTTTCCCTTCCTTTA
TGCTATCAGTTTATTGTCAT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNIVVLISGNGSNLQAIIDACEAKKIKGTLRAVFSNKADAFGLERAREAGIPAQALTADRFDSRDAFDRELIRKIDAYAP
DVVVLAGFMRILSPMFVAHYYGRLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAND
SEDDITARVQTQEHAIYPLVIGWFAQGRLKMRDNAAWLDGRRLPPQGYASDE

Sequences:

>Translated_212_residues
MNIVVLISGNGSNLQAIIDACEAKKIKGTLRAVFSNKADAFGLERAREAGIPAQALTADRFDSRDAFDRELIRKIDAYAP
DVVVLAGFMRILSPMFVAHYYGRLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAND
SEDDITARVQTQEHAIYPLVIGWFAQGRLKMRDNAAWLDGRRLPPQGYASDE
>Mature_212_residues
MNIVVLISGNGSNLQAIIDACEAKKIKGTLRAVFSNKADAFGLERAREAGIPAQALTADRFDSRDAFDRELIRKIDAYAP
DVVVLAGFMRILSPMFVAHYYGRLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAND
SEDDITARVQTQEHAIYPLVIGWFAQGRLKMRDNAAWLDGRRLPPQGYASDE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=198, Percent_Identity=41.4141414141414, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI209869995, Length=198, Percent_Identity=41.4141414141414, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI209869993, Length=198, Percent_Identity=41.4141414141414, Blast_Score=163, Evalue=1e-40,
Organism=Escherichia coli, GI1788846, Length=212, Percent_Identity=87.2641509433962, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1787483, Length=103, Percent_Identity=40.7766990291262, Blast_Score=87, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=178, Percent_Identity=37.0786516853933, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320616, Length=195, Percent_Identity=30.2564102564103, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=48.6631016042781, Blast_Score=186, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23450; Mature: 23450

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVVLISGNGSNLQAIIDACEAKKIKGTLRAVFSNKADAFGLERAREAGIPAQALTADR
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHC
FDSRDAFDRELIRKIDAYAPDVVVLAGFMRILSPMFVAHYYGRLLNIHPSLLPKYPGLHT
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHH
HRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFANDSEDDITARVQTQEHAIYPLV
HHHHHHCCCHHHCCEEEEEEECCCCCCEEEEEECCEEECCCCCCEEEEEECCCCCCHHHH
IGWFAQGRLKMRDNAAWLDGRRLPPQGYASDE
EEECCCCEEEECCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIVVLISGNGSNLQAIIDACEAKKIKGTLRAVFSNKADAFGLERAREAGIPAQALTADR
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHC
FDSRDAFDRELIRKIDAYAPDVVVLAGFMRILSPMFVAHYYGRLLNIHPSLLPKYPGLHT
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHH
HRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFANDSEDDITARVQTQEHAIYPLV
HHHHHHCCCHHHCCEEEEEEECCCCCCEEEEEECCEEECCCCCCEEEEEECCCCCCHHHH
IGWFAQGRLKMRDNAAWLDGRRLPPQGYASDE
EEECCCCEEEECCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]