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The map label for this gene is yfgF [H]

Identifier: 29140882

GI number: 29140882

Start: 420336

End: 422549

Strand: Direct

Name: yfgF [H]

Synonym: t0354

Alternate gene names: 29140882

Gene position: 420336-422549 (Clockwise)

Preceding gene: 29140881

Following gene: 29140887

Centisome position: 8.77

GC content: 47.34

Gene sequence:

>2214_bases
ATGAAGCTGAATAAAACTTATATAAATATCAGAGATAAATGGTGGGGACTACCGCTCATTCTCCCTTCGATTTTACTACC
TGTTTTAAGTAGCGCAAATACTTATGCGCTTACCAGCACTGGAAATGTGGTTCTTTTTTACCTGCCGTTAGCTTTTATGC
TTAGTCTGATGCTCTTTTTCGGCTGGGCAGCACTGCCGGGCATTGTGCTTGCTATCTTCTGGCGCAGATACCCGCAGACA
GGGCTGTATGAAACCCTTTCTGTGACGATGCACTTTATCATTACTATCGTGCTAAGCTGGGGCGGCTATCGTGTCTTTTC
CCCCCGGCGAAACAATGTTTCCCACGGCGACGCCCACCTGTTGTTTCAACGAATGTTCTGGCAGGTATTTTGTTCAGCGA
CATTATTTCTGGTCATTTACCAGTTTGCCGCTTTTGTTGGCATGTACGAAAGTAAAGCCAGCCTGATGGGGGTAATGCCC
TTTAATATCAACACGCTGATTAATTACCAGGCGTTACTGGTCGGTAACCTTGTCGGCGTGCCATTGTGCTATTTTATTAT
TCGTACTCTCCGCAATCCGCTGCACCTGCGGGGGTATTATCAGCAATTAAAGCTACAGATTGATAGCAAGGCCACTAAAA
AAGAGATCGTTATTTGGCTGGCTGTATTGACTACACTAATGTTTATACTGTGTATGCCATTGACGGATAATAGCTCAATA
TTTAGTACCAACTATACGCTTTCGTTATTATTGCCGGTCATGCTGTGGGGCGCGATGCGCTATGGTTATAAGTTTATTTC
AATTATCTGGGCCGTCGTGCTAATTACGTCGATTCATTATTATCAGCGTTATATGCCCTGGTATTCAGGTTATGATACGC
AATTAGCGATTACCTCTTCGAGCTATCTGGTTTTTTCTTTTATTGTTAATTATATGTCTGTACTGGCCACGCGTCAGCGC
GTAGTGAGTGGTCGCGCACGGCGGCTGGCCTATCTTGACCCGGTTGTACATCTTCCCAATTTACGTGCGTTAAACCGCGC
GCTTAAAAATGCACCCTGGTCTACGATCTGTTTTTTACACGTGCCGGGTCTGGAGCTATTGGGTAAAAATTATGGCGTCA
TGCTACGCATCCAGTATAAGCAAAAGCTCTCTCACTGGATTACGCCGATGTTGGCCTCAAATGAGTGCGTCTACCAGATG
TCAGGGCACGACCTGGTGCTGCGTCTTAATACAGAGGCGCATCAGCAGCGTATTGAAGCGCTGGATAAACATATTAAGCA
GTTTCGGTTTATTTGGGATGGACTGCCGCTACAGCCGCCTGTTGGCGTGAGCTACTGCTGCGTGCGTTCTCCGGTTAGCC
ACCTTTATCTGCTGTTGGGCGAATTAAGCACCAGTTCCGATCTTTCGTTGACGACTAACGCGCCGGAGGATTTACAGCGG
CGCGGCGCAATGCACCTGCAGCGCGATTTGAAGGGCAGAATTGCGATGATGAATCGCCTGCAACAGGCGTTGGAGCACGA
TCATTTTTTCCTGATGGCGCAGCCTATTTTCGGTGTTCGTGGGGACGTTTACCATGAAATTTTACTGCGACTCAGGGATG
ACAACGGCGACGTTATCAACCCGGACAATTTTTTACCCGTGGCGCATGAGTTTGGTTTGTCTTCCGCTATCGATCTCTGG
GTGATTGAAAACACGCTACGGTTTATGGCGGCCAGCCGGGAATATATGCCCGCGCATCGTTTTGCCATTAATCTGTCGCC
AACATCGGTATGCCGCGCCCGTTTCCCTGCAGAAGTTAAGCAGCTGCTTACGCAATATCAGGTTGAACCCTGGCAACTGG
TTTTTGAGGTAACGGAAAGCAACTCTCTCACCAATGCTGAGCAGGCGCAGTTGACACTGGGACAGCTACAGCAGTTTGGC
TGTCAGATAGCGATTGATGATTTCGGTACTGGCTACGCCAGCTATGCGCGGCTGAAAAACGTTGACGCCGATATTCTTAA
AATTGACGGCAGTTTTATTCGCAATATAGTGTCAAACAGTCTCGATTATCAGATTGTGGCTTCTATTTGTCATTTAGCGC
GAATGAAAAATATGCAGGTCGTCGCGGAGTATATCGAGAGCGAAGAAATACGAAGCGTAGTGCTGTCGTTGGGGATCGAC
TATCTTCAGGGATATTTAATTGGCGAGCCGCAGCTATTATCTGAGATTCAGTAA

Upstream 100 bases:

>100_bases
ATGACTATCGTGTGATGTATATCGCATCATCAATTTAAAGTCAGGTGGGATGCCTGTCTGTTAACGCCCCTTTGGGATTG
GGGGGTTCTGCTGAGCATCT

Downstream 100 bases:

>100_bases
AGGCAGACGCCGCAGCGCGGCGTCTGATCCACAATTTACGCGGCGATCTCCGGCGAGCTTTCTTCCTCAATATTGAGACG
CCAGCCAGTTACCGCTTCCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 737; Mature: 737

Protein sequence:

>737_residues
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFFGWAALPGIVLAIFWRRYPQT
GLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHLLFQRMFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMP
FNINTLINYQALLVGNLVGVPLCYFIIRTLRNPLHLRGYYQQLKLQIDSKATKKEIVIWLAVLTTLMFILCMPLTDNSSI
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSSSYLVFSFIVNYMSVLATRQR
VVSGRARRLAYLDPVVHLPNLRALNRALKNAPWSTICFLHVPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQM
SGHDLVLRLNTEAHQQRIEALDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVINPDNFLPVAHEFGLSSAIDLW
VIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVKQLLTQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFG
CQIAIDDFGTGYASYARLKNVDADILKIDGSFIRNIVSNSLDYQIVASICHLARMKNMQVVAEYIESEEIRSVVLSLGID
YLQGYLIGEPQLLSEIQ

Sequences:

>Translated_737_residues
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFFGWAALPGIVLAIFWRRYPQT
GLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHLLFQRMFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMP
FNINTLINYQALLVGNLVGVPLCYFIIRTLRNPLHLRGYYQQLKLQIDSKATKKEIVIWLAVLTTLMFILCMPLTDNSSI
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSSSYLVFSFIVNYMSVLATRQR
VVSGRARRLAYLDPVVHLPNLRALNRALKNAPWSTICFLHVPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQM
SGHDLVLRLNTEAHQQRIEALDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVINPDNFLPVAHEFGLSSAIDLW
VIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVKQLLTQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFG
CQIAIDDFGTGYASYARLKNVDADILKIDGSFIRNIVSNSLDYQIVASICHLARMKNMQVVAEYIESEEIRSVVLSLGID
YLQGYLIGEPQLLSEIQ
>Mature_737_residues
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFFGWAALPGIVLAIFWRRYPQT
GLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHLLFQRMFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMP
FNINTLINYQALLVGNLVGVPLCYFIIRTLRNPLHLRGYYQQLKLQIDSKATKKEIVIWLAVLTTLMFILCMPLTDNSSI
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSSSYLVFSFIVNYMSVLATRQR
VVSGRARRLAYLDPVVHLPNLRALNRALKNAPWSTICFLHVPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQM
SGHDLVLRLNTEAHQQRIEALDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVINPDNFLPVAHEFGLSSAIDLW
VIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVKQLLTQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFG
CQIAIDDFGTGYASYARLKNVDADILKIDGSFIRNIVSNSLDYQIVASICHLARMKNMQVVAEYIESEEIRSVVLSLGID
YLQGYLIGEPQLLSEIQ

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=733, Percent_Identity=72.987721691678, Blast_Score=1151, Evalue=0.0,
Organism=Escherichia coli, GI87082096, Length=741, Percent_Identity=28.3400809716599, Blast_Score=310, Evalue=3e-85,
Organism=Escherichia coli, GI1790496, Length=243, Percent_Identity=33.7448559670782, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1787541, Length=435, Percent_Identity=26.8965517241379, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI87081743, Length=248, Percent_Identity=30.241935483871, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI226510982, Length=417, Percent_Identity=26.378896882494, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1786507, Length=231, Percent_Identity=31.1688311688312, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788502, Length=246, Percent_Identity=29.6747967479675, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI87081980, Length=249, Percent_Identity=28.9156626506024, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081921, Length=463, Percent_Identity=24.1900647948164, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87081845, Length=234, Percent_Identity=28.2051282051282, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787055, Length=247, Percent_Identity=26.7206477732794, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1789650, Length=246, Percent_Identity=21.9512195121951, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 84304; Mature: 84304

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFF
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHHHHH
GWAALPGIVLAIFWRRYPQTGLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
LFQRMFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMPFNINTLINYQALLVGNLVGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCHHHHHHHHHHHHHHHHHH
PLCYFIIRTLRNPLHLRGYYQQLKLQIDSKATKKEIVIWLAVLTTLMFILCMPLTDNSSI
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCE
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEECC
SYLVFSFIVNYMSVLATRQRVVSGRARRLAYLDPVVHLPNLRALNRALKNAPWSTICFLH
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHCCCCHHHHHHHHHCCCCCEEEEEE
VPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQMSGHDLVLRLNTEAHQQRIEA
CCCHHHHCCCCCEEEEEEHHHHHHHHHCHHHCCCCEEEEECCCEEEEEECCHHHHHHHHH
LDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHCCCCCCCCEEECCCCHHHHH
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVIN
HCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEC
PDNFLPVAHEFGLSSAIDLWVIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVK
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCHHHH
QLLTQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFGCQIAIDDFGTGYASYARLKN
HHHHHHCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCC
VDADILKIDGSFIRNIVSNSLDYQIVASICHLARMKNMQVVAEYIESEEIRSVVLSLGID
CCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHH
YLQGYLIGEPQLLSEIQ
HHCCEECCCHHHHHCCC
>Mature Secondary Structure
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFF
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHHHHH
GWAALPGIVLAIFWRRYPQTGLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
LFQRMFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMPFNINTLINYQALLVGNLVGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCHHHHHHHHHHHHHHHHHH
PLCYFIIRTLRNPLHLRGYYQQLKLQIDSKATKKEIVIWLAVLTTLMFILCMPLTDNSSI
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCE
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEECC
SYLVFSFIVNYMSVLATRQRVVSGRARRLAYLDPVVHLPNLRALNRALKNAPWSTICFLH
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHCCCCHHHHHHHHHCCCCCEEEEEE
VPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQMSGHDLVLRLNTEAHQQRIEA
CCCHHHHCCCCCEEEEEEHHHHHHHHHCHHHCCCCEEEEECCCEEEEEECCHHHHHHHHH
LDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHCCCCCCCCEEECCCCHHHHH
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVIN
HCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEC
PDNFLPVAHEFGLSSAIDLWVIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVK
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCHHHH
QLLTQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFGCQIAIDDFGTGYASYARLKN
HHHHHHCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCC
VDADILKIDGSFIRNIVSNSLDYQIVASICHLARMKNMQVVAEYIESEEIRSVVLSLGID
CCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHH
YLQGYLIGEPQLLSEIQ
HHCCEECCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]