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The map label for this gene is yfhA [H]

Identifier: 29140828

GI number: 29140828

Start: 335351

End: 336688

Strand: Direct

Name: yfhA [H]

Synonym: t0293

Alternate gene names: 29140828

Gene position: 335351-336688 (Clockwise)

Preceding gene: 29140827

Following gene: 29140829

Centisome position: 7.0

GC content: 55.46

Gene sequence:

>1338_bases
ATGATAAGCCGTAAACCGGCGCACCTGCTGCTGGTCGATGACGATCCCGGATTACTGAAATTGCTCGGTATGCGCCTGAC
CAGTGAAGGATACAGCGTCGTGACCGCAGAGAGCGGCCAGGAAGGACTACGGGTACTGCATCGGGAAAAAGTGGATCTGG
TAATAAGCGACCTGCGCATGGACGAAATGGATGGTATGCAACTGTTCACAGAAATTCAGAAAGTACAGCCGGGAATGCCG
GTTATTATTCTGACGGCGCATGGTTCTATTCCGGATGCGGTGGCCGCTACCCAGAAGGGCGTATTCAGCTTTTTGACGAA
GCCGATCGACAGAGACGCGTTATATAAAGCGATAGATGAAGCGCTGGAGCAGTCCGCTCCCGCGACGGACGATAGCTGGC
GCAACGCTATCGTTACCCGTAGCCCGCTGATGCTGCGACTGCTTGAACAGGCGCGCATGGTCGCGCAGTCGGATGTCAGC
GTATTGATTAACGGACAAAGCGGCACCGGGAAAGAGATTTTCGCACAGGCGATTCATAATGCCAGCCCGCGCAATAACAA
GCCGTTTGTCGCCATCAACTGCGGCGCGTTACCGGAACAGTTGCTGGAGTCCGAATTATTCGGCCATGCGCGCGGCGCCT
TTACCGGCGCAGTGAGCAATCGTGAAGGGTTGTTCCAGGCGGCGGAAGGCGGCACGCTGTTTCTGGATGAAATTGGCGAT
ATGCCCGCGCCGTTACAGGTGAAGCTGCTGCGGGTGTTACAGGAGCGCAAAGTCAGGCCGCTGGGCAGCAATCGCGATAT
TGATATCGACGTGCGAATTATTTCCGCGACGCACCGCGATCTGCCGAAGGCGATGGCGCGGGGCGAATTTCGTGAAGATT
TGTACTACCGGCTGAATGTCGTGAGCCTGAAAATTCCGGCGCTGGCGGAGCGTACCGAGGATATTCCGCTGCTGGCTAAT
CATCTGCTCAGACAGTCGGCACAGCGGCATAAACCGTTTGTTCGCGCCTTTTCTACTGATGCAATGAAACGGCTGATGAC
GGCAAGCTGGCCGGGCAACGTGCGCCAACTGGTCAACGTCATTGAACAATGTGTGGCGTTAACCTCTTCGCCGGTGATCA
GCGATGCATTGGTCGAGCAGGCGCTGGAAGGCGAAAACACCGCGCTGCCAACTTTTGCCGAAGCGCGAAACCAATTTGAA
CTGAACTATCTGCGCAAGTTACTGCAAATTACCAAAGGTAACGTGACGCACGCGGCAAGGATGGCGGGGCGCAACCGCAC
GGAATTCTATAAGCTACTCTCCCGTCACGAACTGGATGCGAATGATTTCAAAGAGTAG

Upstream 100 bases:

>100_bases
CGGCAGGGAATTACAACGCCGATACGCCGCATACGAATGACAAACCCGCGACGTCTGAAGATGGCGCGGCGCCGTCGCCG
TCACAAGATGAGGTAACGCC

Downstream 100 bases:

>100_bases
TTTTCATGCCGTAAAGTAGCGCGAAATATGTTACGTTTAACCGATCAAAACACAGGCGACCTTTTCAAGGAATAGCATGA
AAAAGATTGATGCGATTATT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MISRKPAHLLLVDDDPGLLKLLGMRLTSEGYSVVTAESGQEGLRVLHREKVDLVISDLRMDEMDGMQLFTEIQKVQPGMP
VIILTAHGSIPDAVAATQKGVFSFLTKPIDRDALYKAIDEALEQSAPATDDSWRNAIVTRSPLMLRLLEQARMVAQSDVS
VLINGQSGTGKEIFAQAIHNASPRNNKPFVAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGD
MPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISATHRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLAN
HLLRQSAQRHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFAEARNQFE
LNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE

Sequences:

>Translated_445_residues
MISRKPAHLLLVDDDPGLLKLLGMRLTSEGYSVVTAESGQEGLRVLHREKVDLVISDLRMDEMDGMQLFTEIQKVQPGMP
VIILTAHGSIPDAVAATQKGVFSFLTKPIDRDALYKAIDEALEQSAPATDDSWRNAIVTRSPLMLRLLEQARMVAQSDVS
VLINGQSGTGKEIFAQAIHNASPRNNKPFVAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGD
MPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISATHRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLAN
HLLRQSAQRHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFAEARNQFE
LNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE
>Mature_445_residues
MISRKPAHLLLVDDDPGLLKLLGMRLTSEGYSVVTAESGQEGLRVLHREKVDLVISDLRMDEMDGMQLFTEIQKVQPGMP
VIILTAHGSIPDAVAATQKGVFSFLTKPIDRDALYKAIDEALEQSAPATDDSWRNAIVTRSPLMLRLLEQARMVAQSDVS
VLINGQSGTGKEIFAQAIHNASPRNNKPFVAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGD
MPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISATHRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLAN
HLLRQSAQRHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFAEARNQFE
LNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=95.2702702702703, Blast_Score=851, Evalue=0.0,
Organism=Escherichia coli, GI1790437, Length=439, Percent_Identity=44.6469248291572, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1788550, Length=454, Percent_Identity=40.3083700440529, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=38.1465517241379, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI87082117, Length=232, Percent_Identity=55.1724137931034, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1789087, Length=313, Percent_Identity=47.2843450479233, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1789233, Length=252, Percent_Identity=48.015873015873, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=40.5063291139241, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786524, Length=377, Percent_Identity=38.9920424403183, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=37.2670807453416, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1787583, Length=239, Percent_Identity=42.6778242677824, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789828, Length=282, Percent_Identity=36.8794326241135, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87081858, Length=314, Percent_Identity=33.4394904458599, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI145693157, Length=110, Percent_Identity=34.5454545454545, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49386; Mature: 49386

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRKPAHLLLVDDDPGLLKLLGMRLTSEGYSVVTAESGQEGLRVLHREKVDLVISDLRM
CCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCC
DEMDGMQLFTEIQKVQPGMPVIILTAHGSIPDAVAATQKGVFSFLTKPIDRDALYKAIDE
CCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALEQSAPATDDSWRNAIVTRSPLMLRLLEQARMVAQSDVSVLINGQSGTGKEIFAQAIHN
HHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHC
ASPRNNKPFVAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGD
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCEEECCCCEEEHHHHCC
MPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISATHRDLPKAMARGEFREDLYYRLNV
CCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHCCCHHHHHHHEEEE
VSLKIPALAERTEDIPLLANHLLRQSAQRHKPFVRAFSTDAMKRLMTASWPGNVRQLVNV
EEEECCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IEQCVALTSSPVISDALVEQALEGENTALPTFAEARNQFELNYLRKLLQITKGNVTHAAR
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHH
MAGRNRTEFYKLLSRHELDANDFKE
HHCCCHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure
MISRKPAHLLLVDDDPGLLKLLGMRLTSEGYSVVTAESGQEGLRVLHREKVDLVISDLRM
CCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCC
DEMDGMQLFTEIQKVQPGMPVIILTAHGSIPDAVAATQKGVFSFLTKPIDRDALYKAIDE
CCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALEQSAPATDDSWRNAIVTRSPLMLRLLEQARMVAQSDVSVLINGQSGTGKEIFAQAIHN
HHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHC
ASPRNNKPFVAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGD
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCEEECCCCEEEHHHHCC
MPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISATHRDLPKAMARGEFREDLYYRLNV
CCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHCCCHHHHHHHEEEE
VSLKIPALAERTEDIPLLANHLLRQSAQRHKPFVRAFSTDAMKRLMTASWPGNVRQLVNV
EEEECCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IEQCVALTSSPVISDALVEQALEGENTALPTFAEARNQFELNYLRKLLQITKGNVTHAAR
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHH
MAGRNRTEFYKLLSRHELDANDFKE
HHCCCHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]