Click here to switch to the map view.

The map label for this gene is yfhK [H]

Identifier: 29140826

GI number: 29140826

Start: 333203

End: 334588

Strand: Direct

Name: yfhK [H]

Synonym: t0292

Alternate gene names: 29140826

Gene position: 333203-334588 (Clockwise)

Preceding gene: 29140825

Following gene: 29140827

Centisome position: 6.95

GC content: 54.69

Gene sequence:

>1386_bases
ATGCTGGCCTTTTTGCTGATCCTGCTGCCGTTGCTGGTTCTGGCCTGGCAGGCGTGGCAAAGCCTTAACGCGTTAAGCGA
TCAGGCTGCGGTGACTAACCGCAGCACGCTTATTGACGCCCGGCGCAGCGAGGCGATGACCAACGTCGCGCTGGAGATGG
AGCGAAGCTACCGACAGTATTGCGTGCTTGACGATCCGACGCTGGCAAAAGTGTATCAAAGCCAGCGTAAGCGCTATAGC
GATATGCTGGATGCCCATGCCGGCGTCTTGCCGGATGATAAGTTGTATCAGGCGTTACGCCAGGATTTAAACGCGCTGGC
GCAATTGCAGTGTAAAGATAGCGGCCCGGAAGCGGCAGCGGCCGCGCGTCTGGAGGCCTTTGCCAACGCGAATACGGAAA
TGGTGCAGGCGACGCGTACTGTGGTTTACTCGCGCGGGCAGCAGTTACAACAAGAAATCGCCGAGCGCGGACAATTTTTC
GGCTGGCAGGCGCTGGTGCTATTTCTGGTGAGCCTGGCGATGGTGCTACTTTTTACCCGCATGATTATCGGGCCGGTGAA
AGGAATTGAACGGATGATTAATCGCCTGGGCGAAGGCCGTTCTTTGGGGAATACCGTCACCTTTACCGGTCCGCGTGAAT
TACGATCGGTGGGGCAGCGCATTATCTGGCTGAGTGAGCGTCTGGCGTGGCTGGAATCCCAACGCCATCAGTTTCTACGT
CACTTGTCCCATGAGCTGAAAACTCCGCTGGCCAGTATGCGCGAGGGGACAGAGCTGCTGGCCGATCAGGTGGTTGGGCC
GCTGACGCCGGAGCAAAAAGAGGTGGTCGATATTCTGGATGACAGCAGTCGTAATTTACAAAAATTGATTGAGCAATTGC
TTGATTACAACCGGAAACTGGTAGACAGTGCCACAGAGCTGGAAGCGGTCGATATCGCGCCGCTTGTGGATATGGTGGTT
TCCGCCCACAGTTTGCCTGCCCGCGCTAAAATGATGCATACCGATGTCGACCTGGAGGCCGAACGCTGCATAGCAGAGCC
CATGTTGCTGATGAGCGTGCTGGATAATCTCTATTCCAACGCTGTGCACTATGGCGCTGAATCCGGTAACATTTGTATCC
GTAGTCGCTCTCAGGGCTCCACGGTTTATATCGATGTGGTTAATAGCGGCGAACCGATTCCGCAAACCGAAAGAGAAATG
ATTTTTGAGCCTTTTTTCCAGGGAAGCCACCAGCGAAAAGGGGCGGTAAAAGGCAGTGGGTTAGGGCTGAGTATCGCCAG
AGATTGTATTCGTCGAATGCAGGGAGAGATCCAGCTGGTGGATGACAAAGCGCAGGAAGTTTGTTTTCGCATCTCGCTGC
CATTGCCTGCATCTGACAAACACTAA

Upstream 100 bases:

>100_bases
TGACGCTCCCCTCGGTTAGCATCTTTTTTATTCTTCTTTTTATGTGAGCGACACGTTGAAGCGCTGGTCTGTTTTCCCCC
GTTCTTTACGACAATTGGTC

Downstream 100 bases:

>100_bases
TATGAATTTAAGCCTGGTGCGTATGTCACACGTTTTCGTCCAAACTATTAAACGATGCCTTTTGCGATGGGGAATACCGG
TGGGCATATCGTGTCTGGCG

Product: sensor kinase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLAFLLILLPLLVLAWQAWQSLNALSDQAAVTNRSTLIDARRSEAMTNVALEMERSYRQYCVLDDPTLAKVYQSQRKRYS
DMLDAHAGVLPDDKLYQALRQDLNALAQLQCKDSGPEAAAAARLEAFANANTEMVQATRTVVYSRGQQLQQEIAERGQFF
GWQALVLFLVSLAMVLLFTRMIIGPVKGIERMINRLGEGRSLGNTVTFTGPRELRSVGQRIIWLSERLAWLESQRHQFLR
HLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVDILDDSSRNLQKLIEQLLDYNRKLVDSATELEAVDIAPLVDMVV
SAHSLPARAKMMHTDVDLEAERCIAEPMLLMSVLDNLYSNAVHYGAESGNICIRSRSQGSTVYIDVVNSGEPIPQTEREM
IFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGEIQLVDDKAQEVCFRISLPLPASDKH

Sequences:

>Translated_461_residues
MLAFLLILLPLLVLAWQAWQSLNALSDQAAVTNRSTLIDARRSEAMTNVALEMERSYRQYCVLDDPTLAKVYQSQRKRYS
DMLDAHAGVLPDDKLYQALRQDLNALAQLQCKDSGPEAAAAARLEAFANANTEMVQATRTVVYSRGQQLQQEIAERGQFF
GWQALVLFLVSLAMVLLFTRMIIGPVKGIERMINRLGEGRSLGNTVTFTGPRELRSVGQRIIWLSERLAWLESQRHQFLR
HLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVDILDDSSRNLQKLIEQLLDYNRKLVDSATELEAVDIAPLVDMVV
SAHSLPARAKMMHTDVDLEAERCIAEPMLLMSVLDNLYSNAVHYGAESGNICIRSRSQGSTVYIDVVNSGEPIPQTEREM
IFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGEIQLVDDKAQEVCFRISLPLPASDKH
>Mature_461_residues
MLAFLLILLPLLVLAWQAWQSLNALSDQAAVTNRSTLIDARRSEAMTNVALEMERSYRQYCVLDDPTLAKVYQSQRKRYS
DMLDAHAGVLPDDKLYQALRQDLNALAQLQCKDSGPEAAAAARLEAFANANTEMVQATRTVVYSRGQQLQQEIAERGQFF
GWQALVLFLVSLAMVLLFTRMIIGPVKGIERMINRLGEGRSLGNTVTFTGPRELRSVGQRIIWLSERLAWLESQRHQFLR
HLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVDILDDSSRNLQKLIEQLLDYNRKLVDSATELEAVDIAPLVDMVV
SAHSLPARAKMMHTDVDLEAERCIAEPMLLMSVLDNLYSNAVHYGAESGNICIRSRSQGSTVYIDVVNSGEPIPQTEREM
IFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGEIQLVDDKAQEVCFRISLPLPASDKH

Specific function: Probable member of a two-component regulatory system yfhA/yfhK. May activate yfhA by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082128, Length=446, Percent_Identity=86.5470852017937, Blast_Score=786, Evalue=0.0,
Organism=Escherichia coli, GI1790861, Length=234, Percent_Identity=32.9059829059829, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI145693157, Length=226, Percent_Identity=29.646017699115, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789808, Length=294, Percent_Identity=23.1292517006803, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786912, Length=250, Percent_Identity=27.2, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87081816, Length=229, Percent_Identity=29.6943231441048, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788393, Length=280, Percent_Identity=26.4285714285714, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=25.2252252252252, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790346, Length=293, Percent_Identity=24.5733788395904, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI48994928, Length=228, Percent_Identity=26.7543859649123, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789149, Length=234, Percent_Identity=26.9230769230769, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786783, Length=303, Percent_Identity=24.4224422442244, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788713, Length=242, Percent_Identity=28.099173553719, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788549, Length=262, Percent_Identity=27.4809160305344, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790300, Length=230, Percent_Identity=29.5652173913043, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786600, Length=229, Percent_Identity=24.8908296943231, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1787894, Length=298, Percent_Identity=25.503355704698, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788279, Length=250, Percent_Identity=26.8, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51630; Mature: 51630

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAFLLILLPLLVLAWQAWQSLNALSDQAAVTNRSTLIDARRSEAMTNVALEMERSYRQY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCE
CVLDDPTLAKVYQSQRKRYSDMLDAHAGVLPDDKLYQALRQDLNALAQLQCKDSGPEAAA
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AARLEAFANANTEMVQATRTVVYSRGQQLQQEIAERGQFFGWQALVLFLVSLAMVLLFTR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MIIGPVKGIERMINRLGEGRSLGNTVTFTGPRELRSVGQRIIWLSERLAWLESQRHQFLR
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVDILDDSSRNLQKLIEQLLDYNRKL
HHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VDSATELEAVDIAPLVDMVVSAHSLPARAKMMHTDVDLEAERCIAEPMLLMSVLDNLYSN
HCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVHYGAESGNICIRSRSQGSTVYIDVVNSGEPIPQTEREMIFEPFFQGSHQRKGAVKGSG
HHHCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
LGLSIARDCIRRMQGEIQLVDDKAQEVCFRISLPLPASDKH
CCHHHHHHHHHHHCCCEEEECHHHHHEEEEEECCCCCCCCC
>Mature Secondary Structure
MLAFLLILLPLLVLAWQAWQSLNALSDQAAVTNRSTLIDARRSEAMTNVALEMERSYRQY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCE
CVLDDPTLAKVYQSQRKRYSDMLDAHAGVLPDDKLYQALRQDLNALAQLQCKDSGPEAAA
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AARLEAFANANTEMVQATRTVVYSRGQQLQQEIAERGQFFGWQALVLFLVSLAMVLLFTR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MIIGPVKGIERMINRLGEGRSLGNTVTFTGPRELRSVGQRIIWLSERLAWLESQRHQFLR
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVDILDDSSRNLQKLIEQLLDYNRKL
HHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VDSATELEAVDIAPLVDMVVSAHSLPARAKMMHTDVDLEAERCIAEPMLLMSVLDNLYSN
HCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVHYGAESGNICIRSRSQGSTVYIDVVNSGEPIPQTEREMIFEPFFQGSHQRKGAVKGSG
HHHCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
LGLSIARDCIRRMQGEIQLVDDKAQEVCFRISLPLPASDKH
CCHHHHHHHHHHHCCCEEEECHHHHHEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]