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The map label for this gene is cynR [H]

Identifier: 29140816

GI number: 29140816

Start: 319125

End: 320036

Strand: Reverse

Name: cynR [H]

Synonym: t0282

Alternate gene names: 29140816

Gene position: 320036-319125 (Counterclockwise)

Preceding gene: 29140819

Following gene: 29140815

Centisome position: 6.68

GC content: 43.2

Gene sequence:

>912_bases
ATGAATATCAAACAACTACACTCCTTCCTGATATTGTGTGATGAATTACATTATGGTCGGGCAGCCAGTCGCCTATTTAT
CACTCAGCCATCATTAAGTCAGCAAATCAAACAACTTGAGTCATCACTTAAAACAATATTATTTAAGCGAAAAGGCAGAG
GAATTAAATTAACAAAAGCAGGCATTATTTTGCAACGACACGCTAACAAAATAATGCTGGATTTAAAAAATGCCGAAAAT
GAACTTCTTCCCTATCAGGATCAACAGCGCGATACTATTTCAATCGGGGTGTCAGGCAGCTACCTGGTCCTGCCCGCTTT
TCGCAACTTTATGGCGCACCATCCGGAAATATCTTTAAATGTTAAAGAGTTCTCAACTGAGCAAACCATTAAAAAGCTGA
CGGATAGCGCCGTTGATATTGGCATTGTTTATCGAACAGCGCTCCCTGCCCAGCTAAGCTCGACCATGCTGTTTGAAGAT
GAAATTATCGCTGCAATCCCACTTTCTCATCCTCTGGCGACACGCGAACGTCTGCATCTAAAAGATTTAAACGACCAGCC
CATTATCGTGCTTAATGACTCTCTGCTATTAAGAGGGATCATCACCACAGAATTTAATAACCGCAAAGTCGTTCCTAACG
TCATCTGCGAACTGGATAACCACTACTCATGCCTTGAGTATGCCGAAGCGCAGATTGGCATCGCATTTATTACCCGCTCG
CTAACCCATTTATCGACACCCAAAAATGTTCGCCTGGTTTCACTGGGCATCCCCGCATTCTTTATTCCTGTCATGCTGGT
GCACAGTAATGACCTTTCACTGGATAACGCAACCATCTGCCTGCTCAAGCAGATCAAAAATTTTTACACTGTAGATAACA
AATCAGCGCCAGAAAAGAGCTGGCCCGAGTAG

Upstream 100 bases:

>100_bases
AAATGCACTGATCACAGTTTAAAAAAAGCAAAATAGGCAAACAATTTGGTATTTTTTATAATCGTTATAACAAAACCCAA
TCGATTGGCATTAAATTTTA

Downstream 100 bases:

>100_bases
AAAATAGGCGTATAATGCCCGCCCGATTTGTGTTGTTTCTGAGAATTTCCTGATGGCGCTGTTAATCACCAAAAAATGTA
TCAATTGCGATATGTGCGAG

Product: transcriptional regulator

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNIKQLHSFLILCDELHYGRAASRLFITQPSLSQQIKQLESSLKTILFKRKGRGIKLTKAGIILQRHANKIMLDLKNAEN
ELLPYQDQQRDTISIGVSGSYLVLPAFRNFMAHHPEISLNVKEFSTEQTIKKLTDSAVDIGIVYRTALPAQLSSTMLFED
EIIAAIPLSHPLATRERLHLKDLNDQPIIVLNDSLLLRGIITTEFNNRKVVPNVICELDNHYSCLEYAEAQIGIAFITRS
LTHLSTPKNVRLVSLGIPAFFIPVMLVHSNDLSLDNATICLLKQIKNFYTVDNKSAPEKSWPE

Sequences:

>Translated_303_residues
MNIKQLHSFLILCDELHYGRAASRLFITQPSLSQQIKQLESSLKTILFKRKGRGIKLTKAGIILQRHANKIMLDLKNAEN
ELLPYQDQQRDTISIGVSGSYLVLPAFRNFMAHHPEISLNVKEFSTEQTIKKLTDSAVDIGIVYRTALPAQLSSTMLFED
EIIAAIPLSHPLATRERLHLKDLNDQPIIVLNDSLLLRGIITTEFNNRKVVPNVICELDNHYSCLEYAEAQIGIAFITRS
LTHLSTPKNVRLVSLGIPAFFIPVMLVHSNDLSLDNATICLLKQIKNFYTVDNKSAPEKSWPE
>Mature_303_residues
MNIKQLHSFLILCDELHYGRAASRLFITQPSLSQQIKQLESSLKTILFKRKGRGIKLTKAGIILQRHANKIMLDLKNAEN
ELLPYQDQQRDTISIGVSGSYLVLPAFRNFMAHHPEISLNVKEFSTEQTIKKLTDSAVDIGIVYRTALPAQLSSTMLFED
EIIAAIPLSHPLATRERLHLKDLNDQPIIVLNDSLLLRGIITTEFNNRKVVPNVICELDNHYSCLEYAEAQIGIAFITRS
LTHLSTPKNVRLVSLGIPAFFIPVMLVHSNDLSLDNATICLLKQIKNFYTVDNKSAPEKSWPE

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=229, Percent_Identity=30.1310043668122, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787879, Length=200, Percent_Identity=32.5, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI2367136, Length=269, Percent_Identity=29.368029739777, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1790399, Length=205, Percent_Identity=27.8048780487805, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788887, Length=270, Percent_Identity=24.8148148148148, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788297, Length=244, Percent_Identity=25.4098360655738, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790208, Length=260, Percent_Identity=23.8461538461538, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693105, Length=262, Percent_Identity=24.8091603053435, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787601, Length=158, Percent_Identity=29.1139240506329, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=25.6410256410256, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787589, Length=144, Percent_Identity=28.4722222222222, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34210; Mature: 34210

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKQLHSFLILCDELHYGRAASRLFITQPSLSQQIKQLESSLKTILFKRKGRGIKLTKA
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHH
GIILQRHANKIMLDLKNAENELLPYQDQQRDTISIGVSGSYLVLPAFRNFMAHHPEISLN
CEEEEECCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHCCCCEEEE
VKEFSTEQTIKKLTDSAVDIGIVYRTALPAQLSSTMLFEDEIIAAIPLSHPLATRERLHL
HHHHHHHHHHHHHHHCHHEEEEEEEECCCHHHHHHEEECCCEEEEECCCCCCCHHHHHHE
KDLNDQPIIVLNDSLLLRGIITTEFNNRKVVPNVICELDNHYSCLEYAEAQIGIAFITRS
ECCCCCCEEEECCCEEEEEEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LTHLSTPKNVRLVSLGIPAFFIPVMLVHSNDLSLDNATICLLKQIKNFYTVDNKSAPEKS
HHHCCCCCCEEEEEECCCHHHHHHHEEECCCCCCCCHHHHHHHHHHHHEECCCCCCCCCC
WPE
CCC
>Mature Secondary Structure
MNIKQLHSFLILCDELHYGRAASRLFITQPSLSQQIKQLESSLKTILFKRKGRGIKLTKA
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHH
GIILQRHANKIMLDLKNAENELLPYQDQQRDTISIGVSGSYLVLPAFRNFMAHHPEISLN
CEEEEECCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHCCCCEEEE
VKEFSTEQTIKKLTDSAVDIGIVYRTALPAQLSSTMLFEDEIIAAIPLSHPLATRERLHL
HHHHHHHHHHHHHHHCHHEEEEEEEECCCHHHHHHEEECCCEEEEECCCCCCCHHHHHHE
KDLNDQPIIVLNDSLLLRGIITTEFNNRKVVPNVICELDNHYSCLEYAEAQIGIAFITRS
ECCCCCCEEEECCCEEEEEEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LTHLSTPKNVRLVSLGIPAFFIPVMLVHSNDLSLDNATICLLKQIKNFYTVDNKSAPEKS
HHHCCCCCCEEEEEECCCHHHHHHHEEECCCCCCCCHHHHHHHHHHHHEECCCCCCCCCC
WPE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]