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The map label for this gene is tilS [H]

Identifier: 29140776

GI number: 29140776

Start: 276253

End: 277545

Strand: Direct

Name: tilS [H]

Synonym: t0238

Alternate gene names: 29140776

Gene position: 276253-277545 (Clockwise)

Preceding gene: 29140775

Following gene: 29140779

Centisome position: 5.76

GC content: 59.01

Gene sequence:

>1293_bases
ATGACCACTCTCACGTTGAATACCTCGTTACTCTCTTCTCGCCGTATTCTGGCTGCCTTTAGCGGTGGCCTGGATTCTAC
GGTGCTTCTGCATCAACTGGTGTTGTGGCGAGAACGCCATCCTGACGTCACGCTGCGTGCTATCCATATTCATCACGGTT
TAAGCCCTCATGCTGATAGCTGGGTGCGGCATTGCGAAACCGTATGCGAACGCTGGCAGGTTCCGCTGGTAGTGGAGCGG
GTGACGTTGGCGGATAATGGCCTGGGTATCGAGGCCCATGCGCGTGAAGCGCGCTACCGGGCGTTCGCACAGACGCTATT
GCCTGGCGAAGTGCTGGCAACCGCGCAGCACCTTGACGATCAGTGTGAAACATTCCTGCTGGCGTTAAAGCGCGGCAGCG
GTCCGGCCGGACTTTCCGCGATGGGAGAACGCTCGCCTTTTGCCGGAACTCTGCTTCTTCGCCCGCTGTTGAGAGAAACG
CGGAAGACGCTGGAACAGTGGGCTGTGCGTCATGGCTTATGCTGGATTGAAGATGAAAGCAATCAGGATGACGCCTACGA
TCGCAATTTTTTACGCCTGCGCGCGCTTCCGCTCTTACAGCAGCGCTGGCCGCATTTTCCTGCGGCGGTAGCCCGTAGCG
CGACGCTCTGCGCCGAGCAAGAAAGGCTGCTGGACGAGCTTCTGGCCAGCGATCTTACGGACTGTATCACGACGGAGGGA
ACATTGCGGCTCTCTCCATTAATGTCGATGAGCGATGTGCGGCGGGCGGCGATTTTGCGTCGCTGGCTGGCGATGCGTAA
TGCGCCGATGCCCTCTCGCGACGCGCTGGAACGTATCTGGCAGGAGGTGGCGCTGGCGCGCGACGATGCCTCTCCCTGTT
TACGCTTTGGCGATCACGAGATTCGCCGTTATCAGTCGCAACTGTGGTGGATTAAAACCGTAGCCGGACAACACGAAACG
ACGGTCGCCTGGCCCGTCTGGCAAACACCGCTGGCGCTGCCTGCCGGCTTAGGGACGGTACAGCTCGTTCCTGGCGGCGA
ACTGCGCCGACCGCGAGAGGAAGAATCCGTCAGCATTCGGTTTAAAGCGCCAGGGTTGCTACACATTGTGGGACGTCACG
GCGGACGTAAATTAAAGAAAATCTGGCAGGAGCAGGGGATCCCGCCCTGGCGACGAGACACCACGCCGCTGTTGTTTTAC
GGCGAAACGCTGATTGCGGCTGCGGGTGTTTTTGTGACGCGTGAGGGAGCAGCAGAAGATAAAGAAGGGGTGAGTCTGGT
TTGGCATGCCTGA

Upstream 100 bases:

>100_bases
ATCCGGACGGTTTACCGCTTGAGCTGTATGAGCAGTAAGGCTTGTCATTAAGACGCTCGCCAGGTAACGTTGCCGGGCTA
AGTCACTATAAGCATGGATC

Downstream 100 bases:

>100_bases
TGGCGCTGCGCTTATCAGGCCTACGTATCGCATGGAACGTAGGCCGGATAAGGTGCTTGCACCACCATCCGGCAACAAAA
TACTCAGGATTCGCTAACTA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTTLTLNTSLLSSRRILAAFSGGLDSTVLLHQLVLWRERHPDVTLRAIHIHHGLSPHADSWVRHCETVCERWQVPLVVER
VTLADNGLGIEAHAREARYRAFAQTLLPGEVLATAQHLDDQCETFLLALKRGSGPAGLSAMGERSPFAGTLLLRPLLRET
RKTLEQWAVRHGLCWIEDESNQDDAYDRNFLRLRALPLLQQRWPHFPAAVARSATLCAEQERLLDELLASDLTDCITTEG
TLRLSPLMSMSDVRRAAILRRWLAMRNAPMPSRDALERIWQEVALARDDASPCLRFGDHEIRRYQSQLWWIKTVAGQHET
TVAWPVWQTPLALPAGLGTVQLVPGGELRRPREEESVSIRFKAPGLLHIVGRHGGRKLKKIWQEQGIPPWRRDTTPLLFY
GETLIAAAGVFVTREGAAEDKEGVSLVWHA

Sequences:

>Translated_430_residues
MTTLTLNTSLLSSRRILAAFSGGLDSTVLLHQLVLWRERHPDVTLRAIHIHHGLSPHADSWVRHCETVCERWQVPLVVER
VTLADNGLGIEAHAREARYRAFAQTLLPGEVLATAQHLDDQCETFLLALKRGSGPAGLSAMGERSPFAGTLLLRPLLRET
RKTLEQWAVRHGLCWIEDESNQDDAYDRNFLRLRALPLLQQRWPHFPAAVARSATLCAEQERLLDELLASDLTDCITTEG
TLRLSPLMSMSDVRRAAILRRWLAMRNAPMPSRDALERIWQEVALARDDASPCLRFGDHEIRRYQSQLWWIKTVAGQHET
TVAWPVWQTPLALPAGLGTVQLVPGGELRRPREEESVSIRFKAPGLLHIVGRHGGRKLKKIWQEQGIPPWRRDTTPLLFY
GETLIAAAGVFVTREGAAEDKEGVSLVWHA
>Mature_429_residues
TTLTLNTSLLSSRRILAAFSGGLDSTVLLHQLVLWRERHPDVTLRAIHIHHGLSPHADSWVRHCETVCERWQVPLVVERV
TLADNGLGIEAHAREARYRAFAQTLLPGEVLATAQHLDDQCETFLLALKRGSGPAGLSAMGERSPFAGTLLLRPLLRETR
KTLEQWAVRHGLCWIEDESNQDDAYDRNFLRLRALPLLQQRWPHFPAAVARSATLCAEQERLLDELLASDLTDCITTEGT
LRLSPLMSMSDVRRAAILRRWLAMRNAPMPSRDALERIWQEVALARDDASPCLRFGDHEIRRYQSQLWWIKTVAGQHETT
VAWPVWQTPLALPAGLGTVQLVPGGELRRPREEESVSIRFKAPGLLHIVGRHGGRKLKKIWQEQGIPPWRRDTTPLLFYG
ETLIAAAGVFVTREGAAEDKEGVSLVWHA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=427, Percent_Identity=75.6440281030445, Blast_Score=640, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 48486; Mature: 48355

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLTLNTSLLSSRRILAAFSGGLDSTVLLHQLVLWRERHPDVTLRAIHIHHGLSPHADS
CCEEEEEHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHH
WVRHCETVCERWQVPLVVERVTLADNGLGIEAHAREARYRAFAQTLLPGEVLATAQHLDD
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QCETFLLALKRGSGPAGLSAMGERSPFAGTLLLRPLLRETRKTLEQWAVRHGLCWIEDES
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
NQDDAYDRNFLRLRALPLLQQRWPHFPAAVARSATLCAEQERLLDELLASDLTDCITTEG
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TLRLSPLMSMSDVRRAAILRRWLAMRNAPMPSRDALERIWQEVALARDDASPCLRFGDHE
CEEECCHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCHH
IRRYQSQLWWIKTVAGQHETTVAWPVWQTPLALPAGLGTVQLVPGGELRRPREEESVSIR
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEE
FKAPGLLHIVGRHGGRKLKKIWQEQGIPPWRRDTTPLLFYGETLIAAAGVFVTREGAAED
EECCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHCCEEECCCCCCC
KEGVSLVWHA
CCCCEEEEEC
>Mature Secondary Structure 
TTLTLNTSLLSSRRILAAFSGGLDSTVLLHQLVLWRERHPDVTLRAIHIHHGLSPHADS
CEEEEEHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHH
WVRHCETVCERWQVPLVVERVTLADNGLGIEAHAREARYRAFAQTLLPGEVLATAQHLDD
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QCETFLLALKRGSGPAGLSAMGERSPFAGTLLLRPLLRETRKTLEQWAVRHGLCWIEDES
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
NQDDAYDRNFLRLRALPLLQQRWPHFPAAVARSATLCAEQERLLDELLASDLTDCITTEG
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TLRLSPLMSMSDVRRAAILRRWLAMRNAPMPSRDALERIWQEVALARDDASPCLRFGDHE
CEEECCHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCHH
IRRYQSQLWWIKTVAGQHETTVAWPVWQTPLALPAGLGTVQLVPGGELRRPREEESVSIR
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEE
FKAPGLLHIVGRHGGRKLKKIWQEQGIPPWRRDTTPLLFYGETLIAAAGVFVTREGAAED
EECCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHCCEEECCCCCCC
KEGVSLVWHA
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA