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The map label for this gene is rnhB [H]

Identifier: 29140770

GI number: 29140770

Start: 265801

End: 266397

Strand: Direct

Name: rnhB [H]

Synonym: t0231

Alternate gene names: 29140770

Gene position: 265801-266397 (Clockwise)

Preceding gene: 29140769

Following gene: 29140771

Centisome position: 5.55

GC content: 56.11

Gene sequence:

>597_bases
ATGATCGAATTTGTTTATCCACACACCCATTTAGTGGCGGGTGTGGATGAAGTGGGCCGTGGCCCGCTGGTGGGCGCCGT
AGTGACCGCCGCCGTGATCCTCGATCCGGCGCGTCCGATCGTCGGTCTGAATGATTCCAAAAAATTGTCTGAAAAGCGTC
GTCTGTCATTGTATGACGAGATTAAAGAAAAGGCGTTAAGCTGGAGTCTGGGACGCGCGGAAGCGCATGAAATTGATGAG
CTGAATATTTTGCACGCCACCATGCTCGCCATGCAGCGCGCTGTCGCTGGTCTGCATATTGCGCCAGAATATGTGCTGAT
CGACGGCAATCGCTGTCCGGAGCTGCCTGTGCCGTCAATGGCCGTGGTGAAAGGCGACAGCCGCGTGGCGGAGATTAGCG
CGGCGTCTATTCTGGCGAAAGTCACCCGCGACGCAGAAATGGCGGCGCTGGATATCGTTTTTCCTCAGTATGGCTTTGCG
CAGCACAAAGGCTATCCAACCGCTTTTCATCTGGAAAAGTTGGCCCAGTATGGCGCGACGGCGCACCACCGACGCAGTTT
TGCGCCTGTTAAACGCGCTCTGGGACTTGTGTCCTGA

Upstream 100 bases:

>100_bases
AAACCAGCCATGCGATGCACGACACCTTCCGTGAATTGCATCAGCAGATCCGCTGTAATGCCGATGAGCAGGCGGCAGAT
GCCGTCCTGGAGTTAGCACA

Downstream 100 bases:

>100_bases
TTCTTGTATCGAGAAATCTGAAGATGTCTGAACCACGTTTCGTTCACCTGCGGGTGCACAGCGACTACTCTATGATCGAT
GGGCTGGCGAAGACCGGGCC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIVGLNDSKKLSEKRRLSLYDEIKEKALSWSLGRAEAHEIDE
LNILHATMLAMQRAVAGLHIAPEYVLIDGNRCPELPVPSMAVVKGDSRVAEISAASILAKVTRDAEMAALDIVFPQYGFA
QHKGYPTAFHLEKLAQYGATAHHRRSFAPVKRALGLVS

Sequences:

>Translated_198_residues
MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIVGLNDSKKLSEKRRLSLYDEIKEKALSWSLGRAEAHEIDE
LNILHATMLAMQRAVAGLHIAPEYVLIDGNRCPELPVPSMAVVKGDSRVAEISAASILAKVTRDAEMAALDIVFPQYGFA
QHKGYPTAFHLEKLAQYGATAHHRRSFAPVKRALGLVS
>Mature_198_residues
MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIVGLNDSKKLSEKRRLSLYDEIKEKALSWSLGRAEAHEIDE
LNILHATMLAMQRAVAGLHIAPEYVLIDGNRCPELPVPSMAVVKGDSRVAEISAASILAKVTRDAEMAALDIVFPQYGFA
QHKGYPTAFHLEKLAQYGATAHHRRSFAPVKRALGLVS

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=198, Percent_Identity=92.9292929292929, Blast_Score=376, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 21507; Mature: 21507

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIVGLNDSKKLSEKRRLSLYDE
CCEEECCCHHHHHCHHHHCCCCHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHH
IKEKALSWSLGRAEAHEIDELNILHATMLAMQRAVAGLHIAPEYVLIDGNRCPELPVPSM
HHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHCCEECCCEEEECCCCCCCCCCCCE
AVVKGDSRVAEISAASILAKVTRDAEMAALDIVFPQYGFAQHKGYPTAFHLEKLAQYGAT
EEEECCCHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCCHHHCCCCHHHHHHHHHHHCCC
AHHRRSFAPVKRALGLVS
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIVGLNDSKKLSEKRRLSLYDE
CCEEECCCHHHHHCHHHHCCCCHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHH
IKEKALSWSLGRAEAHEIDELNILHATMLAMQRAVAGLHIAPEYVLIDGNRCPELPVPSM
HHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHCCEECCCEEEECCCCCCCCCCCCE
AVVKGDSRVAEISAASILAKVTRDAEMAALDIVFPQYGFAQHKGYPTAFHLEKLAQYGAT
EEEECCCHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCCHHHCCCCHHHHHHHHHHHCCC
AHHRRSFAPVKRALGLVS
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA