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The map label for this gene is yadF [H]

Identifier: 29140717

GI number: 29140717

Start: 203589

End: 204251

Strand: Reverse

Name: yadF [H]

Synonym: t0176

Alternate gene names: 29140717

Gene position: 204251-203589 (Counterclockwise)

Preceding gene: 29140722

Following gene: 29140715

Centisome position: 4.26

GC content: 50.68

Gene sequence:

>663_bases
ATGAAAGACATAGATACACTCATCAGCAACAATGCACTATGGTCAAAAATGCTGGTGGAAGAGGACCCCGGATTTTTTGA
GAAACTGGCGCAAGCGCAAAAACCGCGCTTTCTATGGATTGGATGTTCCGACAGCCGCGTTCCCGCAGAGCGTTTAACCG
GGCTTGAACCGGGCGAATTGTTTGTTCACCGTAATGTGGCTAACCTGGTTATTCACACCGATCTGAACTGTCTCTCCGTG
GTTCAGTATGCGGTAGATGCTCTGGAAGTTGAGCATATTATCATTTGCGGCCACTCCGGTTGCGGCGGTATCAAGGCTGC
GGTAGAAAACCCTGAGCTGGGCTTGATTAATAACTGGCTGCTGCATATCCGCGACATCTGGCTTAAACATAGCTCGCTGT
TGGGAAAAATGCCCGAAGAGCAACGTCTGGACGCGCTCTATGAATTGAACGTCATGGAGCAGGTCTATAACCTGGGGCAT
TCCACCATTATGCAGTCAGCGTGGAAACGCGGGCAGAATGTGACCATTCACGGCTGGGCGTACAGTATCAATGATGGCCT
GCTGCGCGATCTTGACGTCACCGCCACCAACAGAGAAACGCTGGAGAACGGCTATCATAAGGGTATCTCCGCCCTAAGTC
TGAAATACATTCCCCACCAATAA

Upstream 100 bases:

>100_bases
ACTTACTTTACGTTCTTACACTTTATATATGGTTTAAATAATTACGGAGTTGCCCTATATTAGCCCAACGCACTTATTTG
GTTACAGGTCGTTAACTTCC

Downstream 100 bases:

>100_bases
GCCATTAGCTATAGGCCGGATAAGTCGCTTTAGCGACGCCATCCGGCATTTCTGCGCTTACTCGTCCAGCAGCACCACTT
TACCAACATACGGTAGATGG

Product: carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase 2 [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSDSRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSV
VQYAVDALEVEHIIICGHSGCGGIKAAVENPELGLINNWLLHIRDIWLKHSSLLGKMPEEQRLDALYELNVMEQVYNLGH
STIMQSAWKRGQNVTIHGWAYSINDGLLRDLDVTATNRETLENGYHKGISALSLKYIPHQ

Sequences:

>Translated_220_residues
MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSDSRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSV
VQYAVDALEVEHIIICGHSGCGGIKAAVENPELGLINNWLLHIRDIWLKHSSLLGKMPEEQRLDALYELNVMEQVYNLGH
STIMQSAWKRGQNVTIHGWAYSINDGLLRDLDVTATNRETLENGYHKGISALSLKYIPHQ
>Mature_220_residues
MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSDSRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSV
VQYAVDALEVEHIIICGHSGCGGIKAAVENPELGLINNWLLHIRDIWLKHSSLLGKMPEEQRLDALYELNVMEQVYNLGH
STIMQSAWKRGQNVTIHGWAYSINDGLLRDLDVTATNRETLENGYHKGISALSLKYIPHQ

Specific function: Unknown

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786318, Length=220, Percent_Identity=89.5454545454545, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1786534, Length=203, Percent_Identity=32.512315270936, Blast_Score=97, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324292, Length=200, Percent_Identity=30, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 24794; Mature: 24794

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSDSRVPAERLTGLEPGEL
CCCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHCCCCCCCE
FVHRNVANLVIHTDLNCLSVVQYAVDALEVEHIIICGHSGCGGIKAAVENPELGLINNWL
EEECCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCCCCCCHHHHHH
LHIRDIWLKHSSLLGKMPEEQRLDALYELNVMEQVYNLGHSTIMQSAWKRGQNVTIHGWA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEE
YSINDGLLRDLDVTATNRETLENGYHKGISALSLKYIPHQ
EECCCCCEECCCCCCCCHHHHHHHHHCCHHHEEEECCCCC
>Mature Secondary Structure
MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSDSRVPAERLTGLEPGEL
CCCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHCCCCCCCE
FVHRNVANLVIHTDLNCLSVVQYAVDALEVEHIIICGHSGCGGIKAAVENPELGLINNWL
EEECCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCCCCCCHHHHHH
LHIRDIWLKHSSLLGKMPEEQRLDALYELNVMEQVYNLGHSTIMQSAWKRGQNVTIHGWA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEE
YSINDGLLRDLDVTATNRETLENGYHKGISALSLKYIPHQ
EECCCCCEECCCCCCCCHHHHHHHHHCCHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 10493123 [H]