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The map label for this gene is leuC

Identifier: 29140656

GI number: 29140656

Start: 131078

End: 132478

Strand: Reverse

Name: leuC

Synonym: t0115

Alternate gene names: 29140656

Gene position: 132478-131078 (Counterclockwise)

Preceding gene: 29140657

Following gene: 29140655

Centisome position: 2.76

GC content: 58.03

Gene sequence:

>1401_bases
ATGGCCAAAACGTTATACGAAAAATTATTTGATGCCCACGTGGTCTTTGAGGCGCCAAACGAAACGCCGCTGCTGTACAT
CGATCGCCACCTGGTACACGAAGTGACCTCGCCGCAGGCGTTTGACGGTCTGCGCGCGCACCATCGTCCGGTACGTCAGC
CGGGGAAAACCTTCGCCACGATGGATCACAACGTCTCGACGCAGACTAAAGACATTAATGCTTCTGGTGAAATGGCGCGT
ATCCAGATGCAAGAGCTGATTAAGAACTGTAACGAGTTCGGCGTCGAGCTGTATGACCTGAATCACCCATATCAGGGCAT
CGTCCATGTGATGGGGCCGGAACAGGGCGTTACCCTGCCGGGTATGACCATCGTCTGCGGCGACTCCCACACCGCTACCC
ACGGCGCGTTTGGCGCGTTGGCCTTCGGCATCGGCACTTCTGAGGTAGAACATGTACTGGCGACGCAAACCCTGAAACAG
GGGCGCGCTAAAACCATGAAGATTGAAGTCACGGGCAACGCCGCACCGGGCATTACCGCCAAAGACATCGTGCTGGCGAT
CATCGGTAAAACTGGTAGCGCCGGCGGCACCGGACACGTGGTTGAATTTTGCGGCGACGCTATCCGCGCGCTGAGTATGG
AAGGCCGCATGACGCTGTGCAATATGGCGATTGAGATGGGCGCCAAAGCCGGTCTGGTCGCCCCGGATGAAACCACTTTC
AACTACGTAAAAGGGCGTTTGCACGCGCCGAAGGGCCGCGATTTTGACGAAGCCGTCGAGTACTGGAAAACGCTGAAAAC
CGATGACGGCGCGACCTTTGATACTGTCGTCACCCTGCGAGCAGAAGAGATCGCGCCGCAGGTGACCTGGGGCACTAATC
CGGGCCAGGTGATTTCCGTCACCGACATCATCCCCGATCCCGCCTCTTTTAGCGATCCGGTTGAGCGCGCCAGCGCCGAA
AAAGCGCTGGCTTATATGGGCTTACAGCCGGGCGTACCGTTAACAGACGTTGCTATTGATAAAGTCTTTATCGGCTCTTG
TACCAATTCGCGTATTGAAGATTTGCGCGCGGCGGCGGAAGTCGCCAAAGGGCGCAAAGTTGCGCCTGGCGTGCAGGCGC
TGGTGGTGCCGGGTTCAGGTCCGGTGAAAGCGCAGGCGGAAGCGGAAGGTCTGGACAAGATCTTTATCGAAGCAGGATTT
GAATGGCGCTTACCAGGCTGTTCCATGTGCCTGGCCATGAATAATGACCGCCTGAACCCGGGCGAGCGCTGCGCCTCCAC
CAGCAACCGTAACTTTGAAGGTCGTCAGGGCCGCGGGGGTCGCACGCATTTAGTCAGCCCGGCGATGGCCGCCGCTGCCG
CCGTTACCGGCCACTTCGCCGATATTCGCAGCATCAAATAA

Upstream 100 bases:

>100_bases
AGGGGGTTCGCACCAGCGATTTAGCCAGGGGCGCTGCCGCAGTCAGTACCGATGAGATGGGCGATATTATCGCCCGCTAT
GTCGCAGAAGGGGTGTAATC

Downstream 100 bases:

>100_bases
GGAAACTACCATGGCAGAGAAATTTACCCAGCATACCGGCCTGGTTGTCCCACTGGATGCCGCCAACGTCGATACCGATG
CAATTATCCCTAAACAGTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFATMDHNVSTQTKDINASGEMAR
IQMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
GRAKTMKIEVTGNAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTF
NYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVTLRAEEIAPQVTWGTNPGQVISVTDIIPDPASFSDPVERASAE
KALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAEVAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGF
EWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSIK

Sequences:

>Translated_466_residues
MAKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFATMDHNVSTQTKDINASGEMAR
IQMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
GRAKTMKIEVTGNAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTF
NYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVTLRAEEIAPQVTWGTNPGQVISVTDIIPDPASFSDPVERASAE
KALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAEVAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGF
EWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSIK
>Mature_465_residues
AKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFATMDHNVSTQTKDINASGEMARI
QMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQG
RAKTMKIEVTGNAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTFN
YVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVTLRAEEIAPQVTWGTNPGQVISVTDIIPDPASFSDPVERASAEK
ALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAEVAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFE
WRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSIK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=410, Percent_Identity=26.5853658536585, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI8659555, Length=402, Percent_Identity=27.1144278606965, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI41352693, Length=385, Percent_Identity=27.012987012987, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=94.8497854077253, Blast_Score=907, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=28.8461538461538, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081781, Length=354, Percent_Identity=26.5536723163842, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI2367097, Length=364, Percent_Identity=25.8241758241758, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=412, Percent_Identity=28.6407766990291, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=412, Percent_Identity=28.6407766990291, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=30.6188925081433, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=457, Percent_Identity=27.7899343544858, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=60.0849256900212, Blast_Score=579, Evalue=1e-166,
Organism=Saccharomyces cerevisiae, GI6320440, Length=415, Percent_Identity=26.7469879518072, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6323335, Length=367, Percent_Identity=29.4277929155313, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6322261, Length=402, Percent_Identity=26.6169154228856, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI281365315, Length=410, Percent_Identity=24.8780487804878, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17864292, Length=410, Percent_Identity=24.8780487804878, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161076999, Length=410, Percent_Identity=24.8780487804878, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28571643, Length=409, Percent_Identity=25.4278728606357, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645686, Length=369, Percent_Identity=27.3712737127371, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137564, Length=454, Percent_Identity=25.5506607929515, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC1_SALCH (Q57TE8)

Other databases:

- EMBL:   AE017220
- RefSeq:   YP_215094.1
- ProteinModelPortal:   Q57TE8
- SMR:   Q57TE8
- GeneID:   3332518
- GenomeReviews:   AE017220_GR
- KEGG:   sec:SC0107
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   SENT321314:SCH_0107-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 49816; Mature: 49685

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFAT
CCHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEE
MDHNVSTQTKDINASGEMARIQMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGNAAPGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
KDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTF
HCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHH
NYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVTLRAEEIAPQVTWGTNPGQVISV
HHHHCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCEEEECCCCCCEEEE
TDIIPDPASFSDPVERASAEKALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAE
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEECCCCHHHHHHHHHHH
VAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNP
HHCCCCCCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSIK
HHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFAT
CHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEE
MDHNVSTQTKDINASGEMARIQMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGNAAPGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
KDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTF
HCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHH
NYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVTLRAEEIAPQVTWGTNPGQVISV
HHHHCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCEEEECCCCCCEEEE
TDIIPDPASFSDPVERASAEKALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAE
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEECCCCHHHHHHHHHHH
VAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNP
HHCCCCCCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSIK
HHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA