Click here to switch to the map view.

The map label for this gene is pbpA [H]

Identifier: 28572928

GI number: 28572928

Start: 891053

End: 892510

Strand: Direct

Name: pbpA [H]

Synonym: TW787

Alternate gene names: 28572928

Gene position: 891053-892510 (Clockwise)

Preceding gene: 28572927

Following gene: 28572929

Centisome position: 96.23

GC content: 46.5

Gene sequence:

>1458_bases
ATGTCCAACATGCTATCGGTCAAACATGTAGACAAAAAAAACCGTGACGGGCGCGCAATCGACCCGGCCCTACGGGTTAT
AAGTTATATCGTTGTCGCAATGTTCCTAATGATAATGCTGTCTGCAAGCGGGATTCAAGGTCTTTTTGCTGATAACTTCC
TGAAAGATCCCCGCAACTCGAGAGTGTTGTACGCGGCCGAACGCGTTTATCGCGGGCCGATTTTTGTTGGAAATGAGAGG
GTGGCATATTCCGAAAAGCGAGGAAAGTCCTACAAGCGGTTTTATGCTCATCCGGAACTCTATTCGGCAATAACGGGATA
CTTCAACAATGTGCATGGCAGGTCGGGGCTGGAGAAATATCTCAATTCAGAACTGTCTGGCGCAAATCCTGTGGGACTCA
GCAGAATGTTTGAAGGTGCTGAAAACGGAAGAAGCGTTCTTCTTACGATAAACGAAAAGTTGCAGAAAATTGCATGGCGA
GCACTGGAGGGGTTTAGAGGGTCTGCTGTAATTATTGAACCATCAAGCGGGAAAATTCTGGCCCTTGTATCCCGTCCTGG
ATTTGACGCAAACAAACTCTCAAATGACACGAATGTAGAAGAGTCTTACAAGAAGCTCCTTAAAGACCCGGCTAAGCCTC
TTATAAACAGAGCGGCAAGCTTGTATCATCCTGGATCGGTTTTCAAAATAATAACCATGGCTACAGCGCTTGAGACCGGA
CGCTACGACCTAGACAAAGAAGTAACGGATAGTAACCTCCTGACTTTTCCGGGTCGCAGATTTTTTATAGAAAACTCAGG
AGCGTGTAGCGGGAGAAACAAAATTAACCTGGAACAAGCCCTCGCATTTTCGTGTAATGCCACTTTTGGAAAAATAGCCA
CCGATCTGGGTGGTGAGTTGATTAGCAAGATAGCTGAAAGATTCGGTTTTGGGAAAAAAATTACTATTCCGATACCATTT
CAGGCATCTGAATATCCTGTAACAGGTGACTCTGCACAACTCGCCCAAAGCGGTTTTGGTCAATGGGAAATCTGGGCAAC
CCCACTCCAAATCGCCATGATAAGTGCTGCCATTGCAAACAATGGAAAGCTTATGAAGCCGAATATTGTGCAACGTGTTA
AAGGTCCCGATGCAAATACGATAAAAGAATTTACCCCAGAGATGCTGGGTCGGGTGGCAACCCAGGAGACTGTAAAAAAA
CTTTCAAAGGCTATGGAATCAGTGGTGGAATACGGTACGGCTAGGAGGGGCAAAATTAGAGGAATACAGATTGCGGGAAA
AACGGGTACCGCGGAGGATGGTACAGGAAGAAACACCCTGTGGTTTACCGGCTTTGCCCCGGTTGACAGACCGTTTCTGG
CTGTTAGCATTGTTCTTGAGGAGCAAAAGGGTGACGCCGGTACGATTGCCGTACCGATTGCCAAAAAACTTATAGAAGAA
GCTCTGAACGAGAGGTAG

Upstream 100 bases:

>100_bases
TATCCGTATATCCGATAACCTGCGCAACTCTCCCGCGTTGAATGAATGGAAGACATGAAGGTCCAGATACAACAACCCTG
GCGGCATAGGTAGTAACAAT

Downstream 100 bases:

>100_bases
ACAGTGAGTTTTGACGCCGGTACGGTGCTTGGCAAGAGATACAGACTGATTGCTCGGATTGCGATCGGGGGCATGGGCGA
GGTCTGGAAGGCCCATGATG

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBPA [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSNMLSVKHVDKKNRDGRAIDPALRVISYIVVAMFLMIMLSASGIQGLFADNFLKDPRNSRVLYAAERVYRGPIFVGNER
VAYSEKRGKSYKRFYAHPELYSAITGYFNNVHGRSGLEKYLNSELSGANPVGLSRMFEGAENGRSVLLTINEKLQKIAWR
ALEGFRGSAVIIEPSSGKILALVSRPGFDANKLSNDTNVEESYKKLLKDPAKPLINRAASLYHPGSVFKIITMATALETG
RYDLDKEVTDSNLLTFPGRRFFIENSGACSGRNKINLEQALAFSCNATFGKIATDLGGELISKIAERFGFGKKITIPIPF
QASEYPVTGDSAQLAQSGFGQWEIWATPLQIAMISAAIANNGKLMKPNIVQRVKGPDANTIKEFTPEMLGRVATQETVKK
LSKAMESVVEYGTARRGKIRGIQIAGKTGTAEDGTGRNTLWFTGFAPVDRPFLAVSIVLEEQKGDAGTIAVPIAKKLIEE
ALNER

Sequences:

>Translated_485_residues
MSNMLSVKHVDKKNRDGRAIDPALRVISYIVVAMFLMIMLSASGIQGLFADNFLKDPRNSRVLYAAERVYRGPIFVGNER
VAYSEKRGKSYKRFYAHPELYSAITGYFNNVHGRSGLEKYLNSELSGANPVGLSRMFEGAENGRSVLLTINEKLQKIAWR
ALEGFRGSAVIIEPSSGKILALVSRPGFDANKLSNDTNVEESYKKLLKDPAKPLINRAASLYHPGSVFKIITMATALETG
RYDLDKEVTDSNLLTFPGRRFFIENSGACSGRNKINLEQALAFSCNATFGKIATDLGGELISKIAERFGFGKKITIPIPF
QASEYPVTGDSAQLAQSGFGQWEIWATPLQIAMISAAIANNGKLMKPNIVQRVKGPDANTIKEFTPEMLGRVATQETVKK
LSKAMESVVEYGTARRGKIRGIQIAGKTGTAEDGTGRNTLWFTGFAPVDRPFLAVSIVLEEQKGDAGTIAVPIAKKLIEE
ALNER
>Mature_484_residues
SNMLSVKHVDKKNRDGRAIDPALRVISYIVVAMFLMIMLSASGIQGLFADNFLKDPRNSRVLYAAERVYRGPIFVGNERV
AYSEKRGKSYKRFYAHPELYSAITGYFNNVHGRSGLEKYLNSELSGANPVGLSRMFEGAENGRSVLLTINEKLQKIAWRA
LEGFRGSAVIIEPSSGKILALVSRPGFDANKLSNDTNVEESYKKLLKDPAKPLINRAASLYHPGSVFKIITMATALETGR
YDLDKEVTDSNLLTFPGRRFFIENSGACSGRNKINLEQALAFSCNATFGKIATDLGGELISKIAERFGFGKKITIPIPFQ
ASEYPVTGDSAQLAQSGFGQWEIWATPLQIAMISAAIANNGKLMKPNIVQRVKGPDANTIKEFTPEMLGRVATQETVKKL
SKAMESVVEYGTARRGKIRGIQIAGKTGTAEDGTGRNTLWFTGFAPVDRPFLAVSIVLEEQKGDAGTIAVPIAKKLIEEA
LNER

Specific function: Cell wall formation. Plays an important role in cell division and cell shape maintenance by cross-linking adjacent peptidoglycan chains through transpeptidation [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Probable). Note=Localized to the division site (septum) along with newly synthesized peptidoglycan, between segregated nucleoids (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=415, Percent_Identity=26.0240963855422, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1786272, Length=414, Percent_Identity=24.3961352657005, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 53176; Mature: 53045

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNMLSVKHVDKKNRDGRAIDPALRVISYIVVAMFLMIMLSASGIQGLFADNFLKDPRNS
CCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
RVLYAAERVYRGPIFVGNERVAYSEKRGKSYKRFYAHPELYSAITGYFNNVHGRSGLEKY
EEEEEEHHHHCCCEEECCCCEECCHHCCCHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHH
LNSELSGANPVGLSRMFEGAENGRSVLLTINEKLQKIAWRALEGFRGSAVIIEPSSGKIL
HCCCCCCCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCEE
ALVSRPGFDANKLSNDTNVEESYKKLLKDPAKPLINRAASLYHPGSVFKIITMATALETG
EEEECCCCCHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
RYDLDKEVTDSNLLTFPGRRFFIENSGACSGRNKINLEQALAFSCNATFGKIATDLGGEL
CCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCCHHHHHEEECCCCHHHHHHHHHHHH
ISKIAERFGFGKKITIPIPFQASEYPVTGDSAQLAQSGFGQWEIWATPLQIAMISAAIAN
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCCEEEEECHHHHHHHHHHHHC
NGKLMKPNIVQRVKGPDANTIKEFTPEMLGRVATQETVKKLSKAMESVVEYGTARRGKIR
CCCEECCHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
GIQIAGKTGTAEDGTGRNTLWFTGFAPVDRPFLAVSIVLEEQKGDAGTIAVPIAKKLIEE
EEEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEEECCCCCCEEEHHHHHHHHHH
ALNER
HHCCC
>Mature Secondary Structure 
SNMLSVKHVDKKNRDGRAIDPALRVISYIVVAMFLMIMLSASGIQGLFADNFLKDPRNS
CCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
RVLYAAERVYRGPIFVGNERVAYSEKRGKSYKRFYAHPELYSAITGYFNNVHGRSGLEKY
EEEEEEHHHHCCCEEECCCCEECCHHCCCHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHH
LNSELSGANPVGLSRMFEGAENGRSVLLTINEKLQKIAWRALEGFRGSAVIIEPSSGKIL
HCCCCCCCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCEE
ALVSRPGFDANKLSNDTNVEESYKKLLKDPAKPLINRAASLYHPGSVFKIITMATALETG
EEEECCCCCHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
RYDLDKEVTDSNLLTFPGRRFFIENSGACSGRNKINLEQALAFSCNATFGKIATDLGGEL
CCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCCHHHHHEEECCCCHHHHHHHHHHHH
ISKIAERFGFGKKITIPIPFQASEYPVTGDSAQLAQSGFGQWEIWATPLQIAMISAAIAN
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCCEEEEECHHHHHHHHHHHHC
NGKLMKPNIVQRVKGPDANTIKEFTPEMLGRVATQETVKKLSKAMESVVEYGTARRGKIR
CCCEECCHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
GIQIAGKTGTAEDGTGRNTLWFTGFAPVDRPFLAVSIVLEEQKGDAGTIAVPIAKKLIEE
EEEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEEECCCCCCEEEHHHHHHHHHH
ALNER
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA