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The map label for this gene is thrA2 [H]

Identifier: 28378893

GI number: 28378893

Start: 2086077

End: 2087429

Strand: Direct

Name: thrA2 [H]

Synonym: lp_2308

Alternate gene names: 28378893

Gene position: 2086077-2087429 (Clockwise)

Preceding gene: 28378880

Following gene: 28378910

Centisome position: 63.06

GC content: 45.45

Gene sequence:

>1353_bases
GTGAAAGTAATTAAATTTGGTGGCAGTTCCCTTGCTAGTAGTCAGCAACTGCAAAAAGTCCTCGCGATCGTTGAGGCTGA
TCAAAAACGTAAATATGTGATTGTATCGGCACCTGGTAAACGGTTCGATGGCGACACCAAAGTCACTGATTTACTTATTC
AATATGCCCGTCAAACGATCCATCATCAAGATACGCAAGCCGTTACCGCAGCTATCATTGACCGTTATGCAGAAATCGGT
CACGGGTTTAACGTTCCCGAAGCACAATTGACTCCGATTTTTGAAACGATTCGCAACTTACCAAAACAATCATACGCCGA
CAACACCTACTTGATGGCAGCTTTCAAAGCGCACGGCGAACGTCTCAATGCGCAACTGGTCGCCGCTGTTTTTCAACAAA
GCGGCCTCAACGCTCGCTACCTTGACCCTAAAGATGCGGGAATGGTCGTTACGGACGTTCCTGATGACGCTCAGATTATC
AAGAGTAGTTATGACCAGCTCGCTAAGTGGGCCAACAGCGAACAAATCCTAGTTATCCCCGGATTTTTCGCCTACAATCG
TAACGGCCAAATTTGTACCTTTTCACGCGGTGGCTCCGACATTACTGGCGCGATCATCGCTCGGGGTGTCCATGCTGAAC
GTTATGAAAACTTTACCGATGTCGATGCAATTTACGCGGTCAATCCTAGTTTGGTCGCAAAACCAGCGGCCATTAGTGAA
ATGACGTTTACTGAGATGCGCGAACTTTCTTACGCTGGCTTTTCTGTCTTTCACGACGAAGCGTTGATTCCAGCAATCGA
AGACAACATTACCGTCAACGTCAAGAATACGAACCATCCCGAGGCTCCGGGAACTTTGATTCAGTCAACTCGCGAACCCA
ATCCCCACTACCCGGTGACTGGGATTGCCAGTTCTTCTAGTTTTTGTTCACTATACTTACGTAAGTATCTGCTCAACAAA
GAAGTTGGGCTGGGCCGTAAAATTCTAACGATTTTAGAAAATCATCATATTAGTTATGAACACATGCCATCTGGGATCGA
TGATTTGACCATCATCTTTGATGAACATCAGTTGACGCCCGAATTAGAGAAAACGTTAGTTGCAGAAATCTCAACGGCCA
TCAATCCCGATGAAATTTACTTTACGCACGACTACTCAATCTTAATGCTCGTTGGCGAAAACATGCGCAACCGCGTCGGC
ATCATGTCACGTGCCGCCACCGCATTAGCCAACGACAATATTAAATTAATCATGGTCAATCAAGGCGCATCCGAAATTTC
TATTATGTTCGGTGTACACGACAATGATGCCGATCAAGCCGTTATTGCGTTATATAAAGAATTTTTCGATTAA

Upstream 100 bases:

>100_bases
TCACGATTAAATTAAATTCTCGTGAGTGGCAATTAACATTGCCAAGTCGCGATTTGTCTGCTATTCTTTTCATTATTGAT
AAAATTAAGGAGCGGTTAAT

Downstream 100 bases:

>100_bases
CCCATACGTCCGACTAGTCAACTAGTCGGGCATTTTTTATGGCCGTGTTTTTCATCAATTGTTGTTGAAAGGTGCCGGCA
ATTGACCCCGGATACTCGTT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKVIKFGGSSLASSQQLQKVLAIVEADQKRKYVIVSAPGKRFDGDTKVTDLLIQYARQTIHHQDTQAVTAAIIDRYAEIG
HGFNVPEAQLTPIFETIRNLPKQSYADNTYLMAAFKAHGERLNAQLVAAVFQQSGLNARYLDPKDAGMVVTDVPDDAQII
KSSYDQLAKWANSEQILVIPGFFAYNRNGQICTFSRGGSDITGAIIARGVHAERYENFTDVDAIYAVNPSLVAKPAAISE
MTFTEMRELSYAGFSVFHDEALIPAIEDNITVNVKNTNHPEAPGTLIQSTREPNPHYPVTGIASSSSFCSLYLRKYLLNK
EVGLGRKILTILENHHISYEHMPSGIDDLTIIFDEHQLTPELEKTLVAEISTAINPDEIYFTHDYSILMLVGENMRNRVG
IMSRAATALANDNIKLIMVNQGASEISIMFGVHDNDADQAVIALYKEFFD

Sequences:

>Translated_450_residues
MKVIKFGGSSLASSQQLQKVLAIVEADQKRKYVIVSAPGKRFDGDTKVTDLLIQYARQTIHHQDTQAVTAAIIDRYAEIG
HGFNVPEAQLTPIFETIRNLPKQSYADNTYLMAAFKAHGERLNAQLVAAVFQQSGLNARYLDPKDAGMVVTDVPDDAQII
KSSYDQLAKWANSEQILVIPGFFAYNRNGQICTFSRGGSDITGAIIARGVHAERYENFTDVDAIYAVNPSLVAKPAAISE
MTFTEMRELSYAGFSVFHDEALIPAIEDNITVNVKNTNHPEAPGTLIQSTREPNPHYPVTGIASSSSFCSLYLRKYLLNK
EVGLGRKILTILENHHISYEHMPSGIDDLTIIFDEHQLTPELEKTLVAEISTAINPDEIYFTHDYSILMLVGENMRNRVG
IMSRAATALANDNIKLIMVNQGASEISIMFGVHDNDADQAVIALYKEFFD
>Mature_450_residues
MKVIKFGGSSLASSQQLQKVLAIVEADQKRKYVIVSAPGKRFDGDTKVTDLLIQYARQTIHHQDTQAVTAAIIDRYAEIG
HGFNVPEAQLTPIFETIRNLPKQSYADNTYLMAAFKAHGERLNAQLVAAVFQQSGLNARYLDPKDAGMVVTDVPDDAQII
KSSYDQLAKWANSEQILVIPGFFAYNRNGQICTFSRGGSDITGAIIARGVHAERYENFTDVDAIYAVNPSLVAKPAAISE
MTFTEMRELSYAGFSVFHDEALIPAIEDNITVNVKNTNHPEAPGTLIQSTREPNPHYPVTGIASSSSFCSLYLRKYLLNK
EVGLGRKILTILENHHISYEHMPSGIDDLTIIFDEHQLTPELEKTLVAEISTAINPDEIYFTHDYSILMLVGENMRNRVG
IMSRAATALANDNIKLIMVNQGASEISIMFGVHDNDADQAVIALYKEFFD

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=474, Percent_Identity=25.9493670886076, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1790455, Length=464, Percent_Identity=25.4310344827586, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1790376, Length=293, Percent_Identity=24.5733788395904, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320893, Length=363, Percent_Identity=24.5179063360882, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49851; Mature: 49851

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIKFGGSSLASSQQLQKVLAIVEADQKRKYVIVSAPGKRFDGDTKVTDLLIQYARQTI
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHH
HHQDTQAVTAAIIDRYAEIGHGFNVPEAQLTPIFETIRNLPKQSYADNTYLMAAFKAHGE
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHCCCCEEEEEEEHHCCH
RLNAQLVAAVFQQSGLNARYLDPKDAGMVVTDVPDDAQIIKSSYDQLAKWANSEQILVIP
HHHHHHHHHHHHHCCCCEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEC
GFFAYNRNGQICTFSRGGSDITGAIIARGVHAERYENFTDVDAIYAVNPSLVAKPAAISE
CEEEECCCCCEEEECCCCCCHHHHHHHCCCCHHHHCCCCCCCEEEEECCHHHCCCCHHHH
MTFTEMRELSYAGFSVFHDEALIPAIEDNITVNVKNTNHPEAPGTLIQSTREPNPHYPVT
HHHHHHHHHHHCCCEEECCCCCCEEECCCEEEEEECCCCCCCCCHHHHCCCCCCCCCCEE
GIASSSSFCSLYLRKYLLNKEVGLGRKILTILENHHISYEHMPSGIDDLTIIFDEHQLTP
EECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHCCCCCCCEEEEEECCCCCH
ELEKTLVAEISTAINPDEIYFTHDYSILMLVGENMRNRVGIMSRAATALANDNIKLIMVN
HHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEE
QGASEISIMFGVHDNDADQAVIALYKEFFD
CCCCEEEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVIKFGGSSLASSQQLQKVLAIVEADQKRKYVIVSAPGKRFDGDTKVTDLLIQYARQTI
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHH
HHQDTQAVTAAIIDRYAEIGHGFNVPEAQLTPIFETIRNLPKQSYADNTYLMAAFKAHGE
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHCCCCEEEEEEEHHCCH
RLNAQLVAAVFQQSGLNARYLDPKDAGMVVTDVPDDAQIIKSSYDQLAKWANSEQILVIP
HHHHHHHHHHHHHCCCCEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEC
GFFAYNRNGQICTFSRGGSDITGAIIARGVHAERYENFTDVDAIYAVNPSLVAKPAAISE
CEEEECCCCCEEEECCCCCCHHHHHHHCCCCHHHHCCCCCCCEEEEECCHHHCCCCHHHH
MTFTEMRELSYAGFSVFHDEALIPAIEDNITVNVKNTNHPEAPGTLIQSTREPNPHYPVT
HHHHHHHHHHHCCCEEECCCCCCEEECCCEEEEEECCCCCCCCCHHHHCCCCCCCCCCEE
GIASSSSFCSLYLRKYLLNKEVGLGRKILTILENHHISYEHMPSGIDDLTIIFDEHQLTP
EECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHCCCCCCCEEEEEECCCCCH
ELEKTLVAEISTAINPDEIYFTHDYSILMLVGENMRNRVGIMSRAATALANDNIKLIMVN
HHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEE
QGASEISIMFGVHDNDADQAVIALYKEFFD
CCCCEEEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]