Click here to switch to the map view.

The map label for this gene is brnQ2 [H]

Identifier: 28378812

GI number: 28378812

Start: 1996909

End: 1998258

Strand: Direct

Name: brnQ2 [H]

Synonym: lp_2213

Alternate gene names: 28378812

Gene position: 1996909-1998258 (Clockwise)

Preceding gene: 28378811

Following gene: 28378814

Centisome position: 60.36

GC content: 48.59

Gene sequence:

>1350_bases
ATGAATAACCGTTTATCAGGACGGAATTTACTCTTCATCGGCACCATGTTATTTGGACTATTTTTCGGTGCTGGTAACTT
GATTTTTCCAATTTTTCTGGGCCAGCAAGCCGGCCAACACGTTGGTGCGGCCATTATCGGACTATTGATCACCGGGATTG
GACTTCCATTACTAGGCGTGATCGGCATGGGGCTGACGCGCAGTCAGGGCGTATTTGACCTGGCAACAAACGTTAGCCGA
TCATACGCGTATGTCTTCACCGTACTGCTTTACTTTACCCTCGGGCCACTTTTTGCAATGCCACGATTAGCAACTACGGC
CTTTCAGATTGGACTGGCCCCCTTCTTAACCACCAAATTACAACCCGTGGTGCTATTGGGCTTCTCCGTTGCTTTTTTCG
TCGTCACTTGGTTACTGGCTCGTAATCCCGGTAAGATTATGACCTATGTTGGTAAGTGGCTGACACCCATCTTTTTGCTA
TTATTAGGTGCCTTAATGCTTGTCGCTATCATTAAGCCACTGGGATCACTTAACGTCGCGGCGAGTGGCGCCTATGTCAG
CCATCCAGTTCTAACTGGTTTTACTGAAGGCTACAACACGATGGATGCGTTAGCCTCGCTAGCTTTTGGCATCGTCGTGA
TTGATGCGATTCGCGACCTCGGTGTCCGTGATCCGGGGCAAATCGCAACTGATACCATTAAAGCAGGCCTAATCAGCATG
AGTTTAATGGCAGTCATCTATGGCCTGCTGGCGATTATGGGTACCATGAGCTTAGGTCGCTTTGCTCCAGCTGCCAATGG
GGGGATTACCTTAGCTCAAATTGCCGATGCCTACTTCGGCCGATTCGGTAATGTTTTACTAGCTCTAATTGTCATCATCG
CTTGCTTAAAAACTGCCATTGGCCTCACTTCTGCTTTCGGTGATACGTTGCATGGCATGTTTCCTAAGATTTCCTACCAT
TGGCTGATCACCATCGCTTGTCTCATTCCAGCCATTCTTGCTAACGTTGGGCTAACTGAAATGATTACTTATTCAACCCC
AGTGCTGATGTTTCTCTACCCGCTCGCAATCGTGTTAATCATACTGGCAGTACTGGCACCATGGCTTGGGATGTCCCGCT
GGTTATATGGAATGACAACTGTCTGGACCATGATTCCGGCCATTCTTGCCGGCATTAACGCTATGCCATCCGCCTGGGTA
CAAGCATCCTGGTTGCAAGCTTTGCTTCATCTCAACGCGTGGCTACCGCTTGCCAATCTAGGGTTAGGCTGGAGTGTACC
CGCACTGCTAGGTTTTGGTTTAGGGTGGTTGATCAGCCGTAAACAACCATCTGCTTCTAAATTGCATTAA

Upstream 100 bases:

>100_bases
GTTGCATTACACCGCCAGTTAACGCCCTCACCGACCTTTAATTAATTCTTCACCAAAGCATGCTAAACTATTGCCATGTT
TATTTGAAAGTGGGGATACC

Downstream 100 bases:

>100_bases
TATAGCGTCACATATAAAATAGCGGACCTTCTGATAGCCATCCACGACTTTTCAGAAGGTCCGCTTTAAATTACGAGAGC
CATGCCAATTGACTGGTTGC

Product: branched-chain amino acid transport protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MNNRLSGRNLLFIGTMLFGLFFGAGNLIFPIFLGQQAGQHVGAAIIGLLITGIGLPLLGVIGMGLTRSQGVFDLATNVSR
SYAYVFTVLLYFTLGPLFAMPRLATTAFQIGLAPFLTTKLQPVVLLGFSVAFFVVTWLLARNPGKIMTYVGKWLTPIFLL
LLGALMLVAIIKPLGSLNVAASGAYVSHPVLTGFTEGYNTMDALASLAFGIVVIDAIRDLGVRDPGQIATDTIKAGLISM
SLMAVIYGLLAIMGTMSLGRFAPAANGGITLAQIADAYFGRFGNVLLALIVIIACLKTAIGLTSAFGDTLHGMFPKISYH
WLITIACLIPAILANVGLTEMITYSTPVLMFLYPLAIVLIILAVLAPWLGMSRWLYGMTTVWTMIPAILAGINAMPSAWV
QASWLQALLHLNAWLPLANLGLGWSVPALLGFGLGWLISRKQPSASKLH

Sequences:

>Translated_449_residues
MNNRLSGRNLLFIGTMLFGLFFGAGNLIFPIFLGQQAGQHVGAAIIGLLITGIGLPLLGVIGMGLTRSQGVFDLATNVSR
SYAYVFTVLLYFTLGPLFAMPRLATTAFQIGLAPFLTTKLQPVVLLGFSVAFFVVTWLLARNPGKIMTYVGKWLTPIFLL
LLGALMLVAIIKPLGSLNVAASGAYVSHPVLTGFTEGYNTMDALASLAFGIVVIDAIRDLGVRDPGQIATDTIKAGLISM
SLMAVIYGLLAIMGTMSLGRFAPAANGGITLAQIADAYFGRFGNVLLALIVIIACLKTAIGLTSAFGDTLHGMFPKISYH
WLITIACLIPAILANVGLTEMITYSTPVLMFLYPLAIVLIILAVLAPWLGMSRWLYGMTTVWTMIPAILAGINAMPSAWV
QASWLQALLHLNAWLPLANLGLGWSVPALLGFGLGWLISRKQPSASKLH
>Mature_449_residues
MNNRLSGRNLLFIGTMLFGLFFGAGNLIFPIFLGQQAGQHVGAAIIGLLITGIGLPLLGVIGMGLTRSQGVFDLATNVSR
SYAYVFTVLLYFTLGPLFAMPRLATTAFQIGLAPFLTTKLQPVVLLGFSVAFFVVTWLLARNPGKIMTYVGKWLTPIFLL
LLGALMLVAIIKPLGSLNVAASGAYVSHPVLTGFTEGYNTMDALASLAFGIVVIDAIRDLGVRDPGQIATDTIKAGLISM
SLMAVIYGLLAIMGTMSLGRFAPAANGGITLAQIADAYFGRFGNVLLALIVIIACLKTAIGLTSAFGDTLHGMFPKISYH
WLITIACLIPAILANVGLTEMITYSTPVLMFLYPLAIVLIILAVLAPWLGMSRWLYGMTTVWTMIPAILAGINAMPSAWV
QASWLQALLHLNAWLPLANLGLGWSVPALLGFGLGWLISRKQPSASKLH

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=430, Percent_Identity=34.8837209302326, Blast_Score=225, Evalue=5e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47872; Mature: 47872

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure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CCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8544834 [H]