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The map label for this gene is def

Identifier: 28378768

GI number: 28378768

Start: 1948356

End: 1948916

Strand: Direct

Name: def

Synonym: lp_2155

Alternate gene names: 28378768

Gene position: 1948356-1948916 (Clockwise)

Preceding gene: 28378746

Following gene: 28378770

Centisome position: 58.89

GC content: 43.85

Gene sequence:

>561_bases
TTGATTAAAATGCGCGACATTATCCGCGAAGGTAATCACACGTTACGCGCGGAAGCAAAGCAAGTTAAATTTCCGTTGAG
TGAAGCTGATCAGAAGTTGGCCAATGACATGATGGAATACTTAGAAAACAGCCAGGATCCCGAATTGGCTAAAAAATACG
GACTGCGTGCCGGTGTTGGTTTAGCTGCACCGCAAGTCGACGTTTCGGAACAGATGGCTGCTGTCCTTGTTCCGAGTGAA
AATGAAGATGATGAACCGGTCTTTAAAGACGTCATTATCAATCCCGTGATCATCAGTCACTCCGTCCAACCCGGCGCCTT
AACTGAAGGTGAAGGTTGCTTATCCGTCGACCGTGATATTGCTGGCTACGTTATTCGACACGATCGGATTACGTTACGCT
ACTATAATATGGCTGGTGAAGAGAAAAAGATTCGTCTCAAGAATTATCCGGCCATTGTTTGTCAACATGAAATTGACCAT
CTTCATGGTATTTTATTCTACGACCATATCAATGGCGACAATCCATTTGCCGCTGATGATGATTTAGTTCTAATTTCTTA
A

Upstream 100 bases:

>100_bases
TTCATCTTTTTCATAACGGGCGCTACTTTTTGGCGTAGGCTTATGATAAACTAGATATTAAGTGTAAACTGCGCGATCTT
AATTTTGGAGGTAATTGGAA

Downstream 100 bases:

>100_bases
AGTCATTTAAAAAAGCAGTCCTGAAATGTTCAGGACTGCTTTTTTGGTGCACTCGAATTCAACCGACGCAAATCTTCCAA
ATACTCTAAAGTGGCCTCAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MIKMRDIIREGNHTLRAEAKQVKFPLSEADQKLANDMMEYLENSQDPELAKKYGLRAGVGLAAPQVDVSEQMAAVLVPSE
NEDDEPVFKDVIINPVIISHSVQPGALTEGEGCLSVDRDIAGYVIRHDRITLRYYNMAGEEKKIRLKNYPAIVCQHEIDH
LHGILFYDHINGDNPFAADDDLVLIS

Sequences:

>Translated_186_residues
MIKMRDIIREGNHTLRAEAKQVKFPLSEADQKLANDMMEYLENSQDPELAKKYGLRAGVGLAAPQVDVSEQMAAVLVPSE
NEDDEPVFKDVIINPVIISHSVQPGALTEGEGCLSVDRDIAGYVIRHDRITLRYYNMAGEEKKIRLKNYPAIVCQHEIDH
LHGILFYDHINGDNPFAADDDLVLIS
>Mature_186_residues
MIKMRDIIREGNHTLRAEAKQVKFPLSEADQKLANDMMEYLENSQDPELAKKYGLRAGVGLAAPQVDVSEQMAAVLVPSE
NEDDEPVFKDVIINPVIISHSVQPGALTEGEGCLSVDRDIAGYVIRHDRITLRYYNMAGEEKKIRLKNYPAIVCQHEIDH
LHGILFYDHINGDNPFAADDDLVLIS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=155, Percent_Identity=30.9677419354839, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_LACPL (Q88VB2)

Other databases:

- EMBL:   AL935258
- RefSeq:   NP_785660.1
- ProteinModelPortal:   Q88VB2
- SMR:   Q88VB2
- GeneID:   1063706
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_2155
- NMPDR:   fig|220668.1.peg.1794
- HOGENOM:   HBG665227
- OMA:   PVMGEKL
- ProtClustDB:   PRK00150
- BioCyc:   LPLA220668:LP_2155-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20855; Mature: 20855

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: ACT_SITE 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKMRDIIREGNHTLRAEAKQVKFPLSEADQKLANDMMEYLENSQDPELAKKYGLRAGVG
CCCHHHHHHCCCCEEEECHHHEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
LAAPQVDVSEQMAAVLVPSENEDDEPVFKDVIINPVIISHSVQPGALTEGEGCLSVDRDI
CCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCEEEECCCCCCCCCCCCCCEEECCCC
AGYVIRHDRITLRYYNMAGEEKKIRLKNYPAIVCQHEIDHLHGILFYDHINGDNPFAADD
CCEEEECCEEEEEEECCCCCCCEEEECCCCEEEEEHHHHHHEEEEEEEEECCCCCCCCCC
DLVLIS
CEEEEC
>Mature Secondary Structure
MIKMRDIIREGNHTLRAEAKQVKFPLSEADQKLANDMMEYLENSQDPELAKKYGLRAGVG
CCCHHHHHHCCCCEEEECHHHEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
LAAPQVDVSEQMAAVLVPSENEDDEPVFKDVIINPVIISHSVQPGALTEGEGCLSVDRDI
CCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCEEEECCCCCCCCCCCCCCEEECCCC
AGYVIRHDRITLRYYNMAGEEKKIRLKNYPAIVCQHEIDHLHGILFYDHINGDNPFAADD
CCEEEECCEEEEEEECCCCCCCEEEECCCCEEEEEHHHHHHEEEEEEEEECCCCCCCCCC
DLVLIS
CEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566