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The map label for this gene is ispD

Identifier: 28378484

GI number: 28378484

Start: 1642529

End: 1643230

Strand: Direct

Name: ispD

Synonym: lp_1816

Alternate gene names: 28378484

Gene position: 1642529-1643230 (Clockwise)

Preceding gene: 28378480

Following gene: 28378485

Centisome position: 49.65

GC content: 43.02

Gene sequence:

>702_bases
ATGATTTATGCACAAATATTAGCCGGCGGTAAAGGAACTCGTATGGGCAATGTTCCGATGCCAAAGCAATTCTTGCTGCT
TGCCGACAAACCAATCTTAATCCATACCATCGAAAAATTTACACTTGAAAGTCGCTTTGATGCCATTTTGGTCGTCTGTC
CAGCCGATTGGGTCAGCCATACTGAAGATATCATTAAAAAGTACATCACCGATGAGCGCGTTCACGTTGTCGTTGGTGGG
GCCGACCGTAACGAAACACTAATGAGTGGAATCAACTATATCCAAGATCATTACGGGATTCAAGACGATGATGTTGTTGT
TACCCATGACGCAGTTCGGCCGTTCATCACACAACGTATCATTAACGACAATATCGTCGCCGTTTTAGAAAATAAAGCAG
TCGATACTGTCGTCCCAGCAATCGATACAATCGTCCGCGGTGCTAATGATCAGGTGACCGATATTCCGGTTCGTTCCGAA
ATGTATCAGGGTCAAACCCCTCAAAGTTTTCACATCAAAATATTGATCGACTCATATAACGCACTCAGCTCCGAACAAAA
GGCATCACTGTCTGATTCATGCAAAATCTGCTCGCTGGCTGGTCAAAAGGTTAGTTTAGTACGTGGTGAAAATTATAATT
TTAAAATCACAACGCCGTTTGACTTACGCGTTGCATCAGCATTAGTAGAAAAGAGGTCATAA

Upstream 100 bases:

>100_bases
CCAGGAACGTCACACTTCTTTCATATTTATTCACAAAAAACATAAAATTCTTATTTATCATTATCCACATAATTTAAATA
ATGAATGGGGTCATGTAGAT

Downstream 100 bases:

>100_bases
GATGTTAAATCAAGTTTATCGCTTAGTAAGTCCCCGTCAATTTGAAGTTGAAACGGTCGCCGAAGAGGTAGACGCCGATG
ATTTGATCGTGCGGCCAAAG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MIYAQILAGGKGTRMGNVPMPKQFLLLADKPILIHTIEKFTLESRFDAILVVCPADWVSHTEDIIKKYITDERVHVVVGG
ADRNETLMSGINYIQDHYGIQDDDVVVTHDAVRPFITQRIINDNIVAVLENKAVDTVVPAIDTIVRGANDQVTDIPVRSE
MYQGQTPQSFHIKILIDSYNALSSEQKASLSDSCKICSLAGQKVSLVRGENYNFKITTPFDLRVASALVEKRS

Sequences:

>Translated_233_residues
MIYAQILAGGKGTRMGNVPMPKQFLLLADKPILIHTIEKFTLESRFDAILVVCPADWVSHTEDIIKKYITDERVHVVVGG
ADRNETLMSGINYIQDHYGIQDDDVVVTHDAVRPFITQRIINDNIVAVLENKAVDTVVPAIDTIVRGANDQVTDIPVRSE
MYQGQTPQSFHIKILIDSYNALSSEQKASLSDSCKICSLAGQKVSLVRGENYNFKITTPFDLRVASALVEKRS
>Mature_233_residues
MIYAQILAGGKGTRMGNVPMPKQFLLLADKPILIHTIEKFTLESRFDAILVVCPADWVSHTEDIIKKYITDERVHVVVGG
ADRNETLMSGINYIQDHYGIQDDDVVVTHDAVRPFITQRIINDNIVAVLENKAVDTVVPAIDTIVRGANDQVTDIPVRSE
MYQGQTPQSFHIKILIDSYNALSSEQKASLSDSCKICSLAGQKVSLVRGENYNFKITTPFDLRVASALVEKRS

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=239, Percent_Identity=26.7782426778243, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI157671913, Length=137, Percent_Identity=29.1970802919708, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=24.8908296943231, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_LACPL (Q88W46)

Other databases:

- EMBL:   AL935257
- RefSeq:   NP_785376.1
- ProteinModelPortal:   Q88W46
- SMR:   Q88W46
- GeneID:   1063386
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1816
- NMPDR:   fig|220668.1.peg.1510
- HOGENOM:   HBG672839
- OMA:   GELFNIK
- ProtClustDB:   PRK00155
- BioCyc:   LPLA220668:LP_1816-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25901; Mature: 25901

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYAQILAGGKGTRMGNVPMPKQFLLLADKPILIHTIEKFTLESRFDAILVVCPADWVSH
CEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEHHHHHHHHCCCCEEEEEECCHHHHH
TEDIIKKYITDERVHVVVGGADRNETLMSGINYIQDHYGIQDDDVVVTHDAVRPFITQRI
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHH
INDNIVAVLENKAVDTVVPAIDTIVRGANDQVTDIPVRSEMYQGQTPQSFHIKILIDSYN
HCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCCEEEEEEEECCC
ALSSEQKASLSDSCKICSLAGQKVSLVRGENYNFKITTPFDLRVASALVEKRS
CCCCHHHCCCCCCCCEEECCCCEEEEEECCCCCEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYAQILAGGKGTRMGNVPMPKQFLLLADKPILIHTIEKFTLESRFDAILVVCPADWVSH
CEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEHHHHHHHHCCCCEEEEEECCHHHHH
TEDIIKKYITDERVHVVVGGADRNETLMSGINYIQDHYGIQDDDVVVTHDAVRPFITQRI
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHH
INDNIVAVLENKAVDTVVPAIDTIVRGANDQVTDIPVRSEMYQGQTPQSFHIKILIDSYN
HCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCCEEEEEEEECCC
ALSSEQKASLSDSCKICSLAGQKVSLVRGENYNFKITTPFDLRVASALVEKRS
CCCCHHHCCCCCCCCEEECCCCEEEEEECCCCCEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566