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The map label for this gene is lspA

Identifier: 28378454

GI number: 28378454

Start: 1612454

End: 1612903

Strand: Direct

Name: lspA

Synonym: lp_1780

Alternate gene names: 28378454

Gene position: 1612454-1612903 (Clockwise)

Preceding gene: 28378453

Following gene: 28378455

Centisome position: 48.74

GC content: 40.44

Gene sequence:

>450_bases
ATGTGGATCTATTTAATTTTAATGGTAGCGCTGGTCATTATTGACCAAGTTATCAAAGCAGCGATTGTGAGTCATATTGC
GTTGGGTGCCAGTACGAGTATTGTTACCGGGTTATTATCACTCACAAATTTACATAATAACGGGGCTGCTTGGAGTATCT
TAGAAGGCAAGATGTGGTTCTTCTACTTGATTTCAGTGATTGCCTTGATCGTGATGGGCTACTTGCTGTGGCGCTTACGT
GGCAAATGGCTATATGAGGTTGGTATTTCACTGATGATTGCGGGAACTTTGGGGAATTTCATTGACCGGTTACGTATTGG
GTACGTCGTCGATATGTTCCAACTTGATTTTATTAATTTTCCAATTTTTAACTTTGCGGATTCATGTTTAACCGTGGGCG
TCATTTTTATCTTGATCGGCGTGCTGCGGGATGATAGTTTTGAAAAATAA

Upstream 100 bases:

>100_bases
AGTCTGCAATAAAAACCGCGCTTTTCCTGCCGGAGTTTCGTACAAGGACGACGTCTTTTGGTATAATTAAACACAGCTTA
GCGAAATGTGAGGACAGAAT

Downstream 100 bases:

>100_bases
GTTACAGGGAGCTGATAACGTGGCAGGAGCGACCACGGAACAACAATTCACGATTACTGACCAAACAGGGCGCTTGGATA
AAGTGTTAGCGGCACTACAG

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MWIYLILMVALVIIDQVIKAAIVSHIALGASTSIVTGLLSLTNLHNNGAAWSILEGKMWFFYLISVIALIVMGYLLWRLR
GKWLYEVGISLMIAGTLGNFIDRLRIGYVVDMFQLDFINFPIFNFADSCLTVGVIFILIGVLRDDSFEK

Sequences:

>Translated_149_residues
MWIYLILMVALVIIDQVIKAAIVSHIALGASTSIVTGLLSLTNLHNNGAAWSILEGKMWFFYLISVIALIVMGYLLWRLR
GKWLYEVGISLMIAGTLGNFIDRLRIGYVVDMFQLDFINFPIFNFADSCLTVGVIFILIGVLRDDSFEK
>Mature_149_residues
MWIYLILMVALVIIDQVIKAAIVSHIALGASTSIVTGLLSLTNLHNNGAAWSILEGKMWFFYLISVIALIVMGYLLWRLR
GKWLYEVGISLMIAGTLGNFIDRLRIGYVVDMFQLDFINFPIFNFADSCLTVGVIFILIGVLRDDSFEK

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=150, Percent_Identity=34, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_LACPL (Q88W75)

Other databases:

- EMBL:   AL935257
- RefSeq:   NP_785346.1
- MEROPS:   A08.001
- GeneID:   1064177
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1780
- NMPDR:   fig|220668.1.peg.1480
- HOGENOM:   HBG724422
- OMA:   SYVVDMI
- ProtClustDB:   PRK14797
- BioCyc:   LPLA220668:LP_1780-MONOMER
- BRENDA:   3.4.23.36
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 16765; Mature: 16765

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 102-102 ACT_SITE 127-127

Signals:

None

Transmembrane regions:

HASH(0xd0cab9c)-; HASH(0xca7114c)-; HASH(0xe3e8cbc)-; HASH(0xce9d7b8)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWIYLILMVALVIIDQVIKAAIVSHIALGASTSIVTGLLSLTNLHNNGAAWSILEGKMWF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
FYLISVIALIVMGYLLWRLRGKWLYEVGISLMIAGTLGNFIDRLRIGYVVDMFQLDFINF
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIFNFADSCLTVGVIFILIGVLRDDSFEK
CHHHHHHHHHHHHHHHHHHHHHHCCCCCH
>Mature Secondary Structure
MWIYLILMVALVIIDQVIKAAIVSHIALGASTSIVTGLLSLTNLHNNGAAWSILEGKMWF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
FYLISVIALIVMGYLLWRLRGKWLYEVGISLMIAGTLGNFIDRLRIGYVVDMFQLDFINF
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIFNFADSCLTVGVIFILIGVLRDDSFEK
CHHHHHHHHHHHHHHHHHHHHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12566566