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The map label for this gene is holB [H]

Identifier: 28377572

GI number: 28377572

Start: 647296

End: 648315

Strand: Direct

Name: holB [H]

Synonym: lp_0705

Alternate gene names: 28377572

Gene position: 647296-648315 (Clockwise)

Preceding gene: 28377571

Following gene: 28377573

Centisome position: 19.57

GC content: 48.73

Gene sequence:

>1020_bases
ATGGCAACAACCGAGTCATTTGCGCAAACGTTAGCGGCGAAACAGCCCCGCTTATTAGCGGCATTTAAACACATCATCGC
CCAGAACCATCTGGCCCACGCGTACTTATTCGCGGGGATGGAAGGCGCTGGACAACCAGAACTGGCCCATTGGATTGCGC
AACGGTTGTTCTGCCTGCATATTAATGATGGTGAACCAGATGGCACTTGTGAGGAGTGCGTCCGGATTGCTAATGGGTCG
CACCCTGATATTGTGACAGTGGCTCCCGAGGGTCAGCGAATTCACGTTGATCAGGTTCGTTACTTAAAAGCTGAATTCTC
TAAGAGTGCGGTCGAAGGTAACCGGAAACTGTTCATCATTAATGATGCGGAGAAGATGACAGCGAGTGCTGCCAATAGCT
TATTGAAGTTTATTGAGGAGCCAAGTGGTAACGTCACGGCCTTGTTATTAACGACTAATAAGCAACTGATGCTACCAACG
ATTATTTCACGAACCCAGGTGATCGAATTCCCACCATTAAATGCGCAGGCGTTACAGCAGACGTTTGAACAGGCCGGGAT
TGCGCCAAACCAAGCGCAGATGTTACGGGGCCTGACAAATAGCGTGACCCAGGCGCAGGCGTTGCTAGCGGATGATTGGT
TACCACAAGCTGCGCAAGCATTGTGGCGGTGGTTTGACCAGGCTGTTAAGGGTGACCCACGGAGTTTTGTGGCTGTGCAG
GTCAATTTAGTTGGTTTGGCTAAGGACGCTGACCATCAGCTGGTTATGTTGGATTTAATTGCGGCCTTGTTCCAAGATCT
ATTGCAATTACATTTTGAGGTGGCAACGACAGCGTTAACCTTTGGTCAGTACCAAAAAGAATTGGCCGGGTTAACGGCTG
AAGTCAGCGATGAGCAGTTGATTGCGGCGACAGAACTAGCTTTAACGGCCAAGCGCCAGCTAGCTAGCAATATTAGTTTT
CAAAATGTCTTAGAAGGCTTAACGTTAAAGTTATGGCCGATTTTTCAAACAAACGCTTAA

Upstream 100 bases:

>100_bases
GGCGCAGCTGCCTATCCAGTTGAAGTTCAAGTCGGTGGCGCCACGGTCTTTGTTCTACCAATTGAACAATTTCACCAATT
TTAATGAAGGATGATTATTA

Downstream 100 bases:

>100_bases
ATAAAGGAAAACGAGGGTGAGCGCGTGGATAAGCGTGATTTATACGATAGTTTCGGTGAGATGGAACAACAGATGCAACA
AATGCTAGACAAAATGGCTA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MATTESFAQTLAAKQPRLLAAFKHIIAQNHLAHAYLFAGMEGAGQPELAHWIAQRLFCLHINDGEPDGTCEECVRIANGS
HPDIVTVAPEGQRIHVDQVRYLKAEFSKSAVEGNRKLFIINDAEKMTASAANSLLKFIEEPSGNVTALLLTTNKQLMLPT
IISRTQVIEFPPLNAQALQQTFEQAGIAPNQAQMLRGLTNSVTQAQALLADDWLPQAAQALWRWFDQAVKGDPRSFVAVQ
VNLVGLAKDADHQLVMLDLIAALFQDLLQLHFEVATTALTFGQYQKELAGLTAEVSDEQLIAATELALTAKRQLASNISF
QNVLEGLTLKLWPIFQTNA

Sequences:

>Translated_339_residues
MATTESFAQTLAAKQPRLLAAFKHIIAQNHLAHAYLFAGMEGAGQPELAHWIAQRLFCLHINDGEPDGTCEECVRIANGS
HPDIVTVAPEGQRIHVDQVRYLKAEFSKSAVEGNRKLFIINDAEKMTASAANSLLKFIEEPSGNVTALLLTTNKQLMLPT
IISRTQVIEFPPLNAQALQQTFEQAGIAPNQAQMLRGLTNSVTQAQALLADDWLPQAAQALWRWFDQAVKGDPRSFVAVQ
VNLVGLAKDADHQLVMLDLIAALFQDLLQLHFEVATTALTFGQYQKELAGLTAEVSDEQLIAATELALTAKRQLASNISF
QNVLEGLTLKLWPIFQTNA
>Mature_338_residues
ATTESFAQTLAAKQPRLLAAFKHIIAQNHLAHAYLFAGMEGAGQPELAHWIAQRLFCLHINDGEPDGTCEECVRIANGSH
PDIVTVAPEGQRIHVDQVRYLKAEFSKSAVEGNRKLFIINDAEKMTASAANSLLKFIEEPSGNVTALLLTTNKQLMLPTI
ISRTQVIEFPPLNAQALQQTFEQAGIAPNQAQMLRGLTNSVTQAQALLADDWLPQAAQALWRWFDQAVKGDPRSFVAVQV
NLVGLAKDADHQLVMLDLIAALFQDLLQLHFEVATTALTFGQYQKELAGLTAEVSDEQLIAATELALTAKRQLASNISFQ
NVLEGLTLKLWPIFQTNA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=180, Percent_Identity=27.7777777777778, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37186; Mature: 37055

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTESFAQTLAAKQPRLLAAFKHIIAQNHLAHAYLFAGMEGAGQPELAHWIAQRLFCLH
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEE
INDGEPDGTCEECVRIANGSHPDIVTVAPEGQRIHVDQVRYLKAEFSKSAVEGNRKLFII
ECCCCCCCCHHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEE
NDAEKMTASAANSLLKFIEEPSGNVTALLLTTNKQLMLPTIISRTQVIEFPPLNAQALQQ
ECHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEHHHHHCCCEEEECCCCCHHHHHH
TFEQAGIAPNQAQMLRGLTNSVTQAQALLADDWLPQAAQALWRWFDQAVKGDPRSFVAVQ
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
VNLVGLAKDADHQLVMLDLIAALFQDLLQLHFEVATTALTFGQYQKELAGLTAEVSDEQL
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
IAATELALTAKRQLASNISFQNVLEGLTLKLWPIFQTNA
HHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEEEEECCC
>Mature Secondary Structure 
ATTESFAQTLAAKQPRLLAAFKHIIAQNHLAHAYLFAGMEGAGQPELAHWIAQRLFCLH
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEE
INDGEPDGTCEECVRIANGSHPDIVTVAPEGQRIHVDQVRYLKAEFSKSAVEGNRKLFII
ECCCCCCCCHHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEE
NDAEKMTASAANSLLKFIEEPSGNVTALLLTTNKQLMLPTIISRTQVIEFPPLNAQALQQ
ECHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEHHHHHCCCEEEECCCCCHHHHHH
TFEQAGIAPNQAQMLRGLTNSVTQAQALLADDWLPQAAQALWRWFDQAVKGDPRSFVAVQ
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
VNLVGLAKDADHQLVMLDLIAALFQDLLQLHFEVATTALTFGQYQKELAGLTAEVSDEQL
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
IAATELALTAKRQLASNISFQNVLEGLTLKLWPIFQTNA
HHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]