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The map label for this gene is thrB

Identifier: 28377454

GI number: 28377454

Start: 530751

End: 531626

Strand: Direct

Name: thrB

Synonym: lp_0572

Alternate gene names: 28377454

Gene position: 530751-531626 (Clockwise)

Preceding gene: 28377453

Following gene: 28377455

Centisome position: 16.04

GC content: 50.57

Gene sequence:

>876_bases
ATGTTAACGATTTCGGTTCCGGCGACTTCCGCTAATCTTGGTCCCGGTTTTGATTCAATCGGTCTGGCGCTGGACATGCA
ACTAACGCTACAAGTGTTGCAACCTAGTGACCATTGGCAGATTGACCATCCCTTTGGGGCGGATGTGCCGACGGATGAAC
GCAACTTAATTATTAAAACGGCGTTGCATTTGGTACCAGATTTACAACCACAGCATTTACAGATGGCTTCAAAAATCCCG
TTAGCGCGCGGATTGGGTAGTAGCTCGACGGCCATCGTAGCCGGCTTAGTGCTGGCAAACGAATTGACCGGCGCAACGCG
CTCTTCTGCTGAGCTACTGGAAGTTGCCACTCAACTGGAGGGTCATCCAGATAACGTTGCACCAGCCCTATTAGGAGGCT
TGGTTGTGGCGACGAACACTGATGGACGCGTCCGTGCGGTGAAGTTACCGTTACCGATGCTTTTTGCAAGTGTCTACGTG
CCTAACGAACCGCTATTGACGACCGCCAGTCGCCAAGCCTTGCCAACTGAATTGGCGTACCACCAAGCCGTTACTGGTAG
TAGTGTGGCAAATACGTTAGTGGCTGCTCTAGCAACTCAAAATTGGGACGTGGCGTTACCGCTGCTAGAACAGGACCAGT
TCCATGAACAGTATCGGGCTAAGTTAGTGCCGGCACTACAAACGGTTAGAGACCATGCCCATGCTCTGGGATTAACGGGG
ACTTATTTGAGTGGTGCTGGGCCGACGGTGATTACTTTGGGTGATTACGGTCAACTCGCTACGTTGCAGGCACAATTGAG
CCAAGATACAACACTCACCGGCCAATTATTCTTATTGCCAATGGATGCAACTGGTGTAAAAGTACAAAAATCTTAA

Upstream 100 bases:

>100_bases
ATGATCAACAGTTAGCAGATATCACTCGTGAAATCGGTGAACAAGAGAATATGCAACTATTAGCGAGTTATAAAGTTTTA
AAGAATGCGTAGGTGAGGAC

Downstream 100 bases:

>100_bases
AATAAGCTTGTCAGACTCGCAAAATTTTGTTATGATATTTTTTGTGAGTTGTGATGCGGCCATGGCGGAATTGGCAGACG
CGCAAGATTAAGGATCTTGT

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLTISVPATSANLGPGFDSIGLALDMQLTLQVLQPSDHWQIDHPFGADVPTDERNLIIKTALHLVPDLQPQHLQMASKIP
LARGLGSSSTAIVAGLVLANELTGATRSSAELLEVATQLEGHPDNVAPALLGGLVVATNTDGRVRAVKLPLPMLFASVYV
PNEPLLTTASRQALPTELAYHQAVTGSSVANTLVAALATQNWDVALPLLEQDQFHEQYRAKLVPALQTVRDHAHALGLTG
TYLSGAGPTVITLGDYGQLATLQAQLSQDTTLTGQLFLLPMDATGVKVQKS

Sequences:

>Translated_291_residues
MLTISVPATSANLGPGFDSIGLALDMQLTLQVLQPSDHWQIDHPFGADVPTDERNLIIKTALHLVPDLQPQHLQMASKIP
LARGLGSSSTAIVAGLVLANELTGATRSSAELLEVATQLEGHPDNVAPALLGGLVVATNTDGRVRAVKLPLPMLFASVYV
PNEPLLTTASRQALPTELAYHQAVTGSSVANTLVAALATQNWDVALPLLEQDQFHEQYRAKLVPALQTVRDHAHALGLTG
TYLSGAGPTVITLGDYGQLATLQAQLSQDTTLTGQLFLLPMDATGVKVQKS
>Mature_291_residues
MLTISVPATSANLGPGFDSIGLALDMQLTLQVLQPSDHWQIDHPFGADVPTDERNLIIKTALHLVPDLQPQHLQMASKIP
LARGLGSSSTAIVAGLVLANELTGATRSSAELLEVATQLEGHPDNVAPALLGGLVVATNTDGRVRAVKLPLPMLFASVYV
PNEPLLTTASRQALPTELAYHQAVTGSSVANTLVAALATQNWDVALPLLEQDQFHEQYRAKLVPALQTVRDHAHALGLTG
TYLSGAGPTVITLGDYGQLATLQAQLSQDTTLTGQLFLLPMDATGVKVQKS

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=311, Percent_Identity=25.08038585209, Blast_Score=91, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6321814, Length=314, Percent_Identity=29.6178343949045, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_LACPL (Q88Z08)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784346.1
- ProteinModelPortal:   Q88Z08
- SMR:   Q88Z08
- GeneID:   1064240
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0572
- NMPDR:   fig|220668.1.peg.480
- HOGENOM:   HBG646290
- OMA:   FDCLGAA
- ProtClustDB:   PRK01212
- BioCyc:   LPLA220668:LP_0572-MONOMER
- BRENDA:   2.7.1.39
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 30702; Mature: 30702

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTISVPATSANLGPGFDSIGLALDMQLTLQVLQPSDHWQIDHPFGADVPTDERNLIIKT
CEEEEECCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCEEECCCCCCCCCCCCCCCHHHH
ALHLVPDLQPQHLQMASKIPLARGLGSSSTAIVAGLVLANELTGATRSSAELLEVATQLE
HHHHCCCCCHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
GHPDNVAPALLGGLVVATNTDGRVRAVKLPLPMLFASVYVPNEPLLTTASRQALPTELAY
CCCCCHHHHHHCCEEEEECCCCCEEEEECCHHHHHHHHCCCCCCCEECCCCCCCCHHHHH
HQAVTGSSVANTLVAALATQNWDVALPLLEQDQFHEQYRAKLVPALQTVRDHAHALGLTG
HHHCCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TYLSGAGPTVITLGDYGQLATLQAQLSQDTTLTGQLFLLPMDATGVKVQKS
EEECCCCCEEEEECCCCCEEHHHHHHCCCCEEEEEEEEEEECCCCEEEECC
>Mature Secondary Structure
MLTISVPATSANLGPGFDSIGLALDMQLTLQVLQPSDHWQIDHPFGADVPTDERNLIIKT
CEEEEECCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCEEECCCCCCCCCCCCCCCHHHH
ALHLVPDLQPQHLQMASKIPLARGLGSSSTAIVAGLVLANELTGATRSSAELLEVATQLE
HHHHCCCCCHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
GHPDNVAPALLGGLVVATNTDGRVRAVKLPLPMLFASVYVPNEPLLTTASRQALPTELAY
CCCCCHHHHHHCCEEEEECCCCCEEEEECCHHHHHHHHCCCCCCCEECCCCCCCCHHHHH
HQAVTGSSVANTLVAALATQNWDVALPLLEQDQFHEQYRAKLVPALQTVRDHAHALGLTG
HHHCCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TYLSGAGPTVITLGDYGQLATLQAQLSQDTTLTGQLFLLPMDATGVKVQKS
EEECCCCCEEEEECCCCCEEHHHHHHCCCCEEEEEEEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566