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The map label for this gene is cysB [H]

Identifier: 28199708

GI number: 28199708

Start: 2154703

End: 2155686

Strand: Reverse

Name: cysB [H]

Synonym: PD1839

Alternate gene names: 28199708

Gene position: 2155686-2154703 (Counterclockwise)

Preceding gene: 28199712

Following gene: 28199705

Centisome position: 85.55

GC content: 55.79

Gene sequence:

>984_bases
ATGACGTTGACTCAACTTCGCTACCTTGTGGCTATTGCCGATGCCGATCTCAATATTACGCTTGCTGCTGCACGTATACA
TGCCACGCAGCCGGGCCTGTCCAAGCAATTGAAAAAGCTAGAGGATGAACTCGGCTTTTTGTTGTTTGTGCGCCGGGGCC
GTAGTTTGGAGTCAGTGACGCCTGCGGGGGAGGAGGTGATTGAGCGGGCGCGGGTGATGTTGGTTGAAGTCAACAACATA
CGTACTTATGCCGCCAATCAGCGCCGTGAGTACCAAGGGCAATTGACGTTGAGTACCACCCACACTCAGGCGCGTTTTGT
GCTGCCGCCTGCCGTGGCGTTGATCAAGCAGGCTTATCCGCAGGTTACTGTGCATTTGCAGCAGGCTGCCGAGAGCGATG
TGCTGGATGAGTTTGGTCAGGGAGGGGCCGATATTGCAATCGTGAGTACTGCGGGCAGCGCGCCTTCATCAGGGCTTGCG
GTCCCTTTGTATCGGTGGCGGCGTTTGGTGATTGTGCCGTGTGGTCATCCTTTGGATACAGTGGGGTGCACTCTGGATTT
GGCAGCGCTGGCGGAGTATCCGTTGGTCAGTTATGACTCTTCAATACGGCCAATGTCTTCGCTGCAACGCGCTTTCGCCA
GTTTAGGGTTGGAGTCGCGCATTGTGTTGACAGCTTTGGATGCTGATTTGATCAAGACCTATGTGCGTGCCGGTCTGGGT
GTGGGCATTCTTGCGGAGATGGCGGTTAATGCGGTCGATACCGATTTGCGTGCTTGGCCGGTACCGGCGCCCATTCCTGA
ATGTATTGCTTGGGCGGTGCTGCCACGTGATCGTGTCTTGCGTGACTACGTGTTGGAGTTGGTGCATGCGCTTGCGCCGC
AGATCGACAAGCGTGATTTGCGGCGTGTGCTCAATGGTCATCAGGAGCCTGACTGGCCGTCGCCTCCTAGCTGGCAATCG
TTGACACAGACCATTACTCATTGA

Upstream 100 bases:

>100_bases
CAGGAAATGACTTCAGGCAGTTGGAAAATAGCCGTTAGTTATTAAGTTCTTCATGGAAGTGATCTACAGTCGTTTTTGGT
TTTGCGATATTTCGCCTGTC

Downstream 100 bases:

>100_bases
GTGGGTTCTCAAGTGGCTGTGTGGGGGCTATGGATGTTCTTGGTGAGTCACGCAGGTGGTGGCAGGGTGTGTGATGTGTT
CACGGGTGCTTGCAGTCACT

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTLTQLRYLVAIADADLNITLAAARIHATQPGLSKQLKKLEDELGFLLFVRRGRSLESVTPAGEEVIERARVMLVEVNNI
RTYAANQRREYQGQLTLSTTHTQARFVLPPAVALIKQAYPQVTVHLQQAAESDVLDEFGQGGADIAIVSTAGSAPSSGLA
VPLYRWRRLVIVPCGHPLDTVGCTLDLAALAEYPLVSYDSSIRPMSSLQRAFASLGLESRIVLTALDADLIKTYVRAGLG
VGILAEMAVNAVDTDLRAWPVPAPIPECIAWAVLPRDRVLRDYVLELVHALAPQIDKRDLRRVLNGHQEPDWPSPPSWQS
LTQTITH

Sequences:

>Translated_327_residues
MTLTQLRYLVAIADADLNITLAAARIHATQPGLSKQLKKLEDELGFLLFVRRGRSLESVTPAGEEVIERARVMLVEVNNI
RTYAANQRREYQGQLTLSTTHTQARFVLPPAVALIKQAYPQVTVHLQQAAESDVLDEFGQGGADIAIVSTAGSAPSSGLA
VPLYRWRRLVIVPCGHPLDTVGCTLDLAALAEYPLVSYDSSIRPMSSLQRAFASLGLESRIVLTALDADLIKTYVRAGLG
VGILAEMAVNAVDTDLRAWPVPAPIPECIAWAVLPRDRVLRDYVLELVHALAPQIDKRDLRRVLNGHQEPDWPSPPSWQS
LTQTITH
>Mature_326_residues
TLTQLRYLVAIADADLNITLAAARIHATQPGLSKQLKKLEDELGFLLFVRRGRSLESVTPAGEEVIERARVMLVEVNNIR
TYAANQRREYQGQLTLSTTHTQARFVLPPAVALIKQAYPQVTVHLQQAAESDVLDEFGQGGADIAIVSTAGSAPSSGLAV
PLYRWRRLVIVPCGHPLDTVGCTLDLAALAEYPLVSYDSSIRPMSSLQRAFASLGLESRIVLTALDADLIKTYVRAGLGV
GILAEMAVNAVDTDLRAWPVPAPIPECIAWAVLPRDRVLRDYVLELVHALAPQIDKRDLRRVLNGHQEPDWPSPPSWQSL
TQTITH

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=298, Percent_Identity=38.9261744966443, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1788296, Length=308, Percent_Identity=33.4415584415584, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1790399, Length=251, Percent_Identity=28.2868525896414, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787879, Length=303, Percent_Identity=27.0627062706271, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367136, Length=244, Percent_Identity=26.6393442622951, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=26.1168384879725, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787806, Length=177, Percent_Identity=29.3785310734463, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI157672245, Length=225, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788297, Length=262, Percent_Identity=23.6641221374046, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=180, Percent_Identity=30.5555555555556, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=147, Percent_Identity=26.530612244898, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35858; Mature: 35727

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTQLRYLVAIADADLNITLAAARIHATQPGLSKQLKKLEDELGFLLFVRRGRSLESVT
CCHHHHEEEHEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCEEEEECCCCCCCCCC
PAGEEVIERARVMLVEVNNIRTYAANQRREYQGQLTLSTTHTQARFVLPPAVALIKQAYP
CCHHHHHHHHHEEEEEECCCEEHHHCCCHHCCCEEEEEECCCCEEEECCHHHHHHHHHCH
QVTVHLQQAAESDVLDEFGQGGADIAIVSTAGSAPSSGLAVPLYRWRRLVIVPCGHPLDT
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEHHHCCEEEEEECCCCHHH
VGCTLDLAALAEYPLVSYDSSIRPMSSLQRAFASLGLESRIVLTALDADLIKTYVRAGLG
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCC
VGILAEMAVNAVDTDLRAWPVPAPIPECIAWAVLPRDRVLRDYVLELVHALAPQIDKRDL
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
RRVLNGHQEPDWPSPPSWQSLTQTITH
HHHHCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TLTQLRYLVAIADADLNITLAAARIHATQPGLSKQLKKLEDELGFLLFVRRGRSLESVT
CHHHHEEEHEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCEEEEECCCCCCCCCC
PAGEEVIERARVMLVEVNNIRTYAANQRREYQGQLTLSTTHTQARFVLPPAVALIKQAYP
CCHHHHHHHHHEEEEEECCCEEHHHCCCHHCCCEEEEEECCCCEEEECCHHHHHHHHHCH
QVTVHLQQAAESDVLDEFGQGGADIAIVSTAGSAPSSGLAVPLYRWRRLVIVPCGHPLDT
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEHHHCCEEEEEECCCCHHH
VGCTLDLAALAEYPLVSYDSSIRPMSSLQRAFASLGLESRIVLTALDADLIKTYVRAGLG
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCC
VGILAEMAVNAVDTDLRAWPVPAPIPECIAWAVLPRDRVLRDYVLELVHALAPQIDKRDL
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
RRVLNGHQEPDWPSPPSWQSLTQTITH
HHHHCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]