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The map label for this gene is nrtB [H]

Identifier: 28199540

GI number: 28199540

Start: 1929629

End: 1931383

Strand: Reverse

Name: nrtB [H]

Synonym: PD1663

Alternate gene names: 28199540

Gene position: 1931383-1929629 (Counterclockwise)

Preceding gene: 28199542

Following gene: 28199539

Centisome position: 76.65

GC content: 55.44

Gene sequence:

>1755_bases
ATGGCGTTCCTTTCTCAAAACCGCCCTGTCCATCCTGCCGTAGCCTCGTTGGTGCGAGCGCAATTACCTAACGTCTATGA
TTTAGTGGCGTTGGCGTTGATATTGCTCCTAGGGATTGCCTTGTTGCATGGTGCCGAAGGCGCAAGCGCGCCGTTGAGCG
CGTTGCCGGTGGAAGCTGTGTCGTTGGATCCAACACACCTGCCTGAGTATGGGTTGCGTACCACCTTGCGGATGTTTGCG
GCGATGACGGCCTCACTGCTGTTTACCTTCGTGGTTGCCACGTTGGCTGCCAAGAGTCAGCGTGCTGAACGACTGATTGT
TCCAGCCTTGGATGTCTTGCAGTCGGTGCCGGTCCTAGGGTTCCTGACATTTACTGTGACGTTCTTTCTCGGACTGTTCC
CGGGTAGCCAACTGGGTGCCGAATGTGCCTCGGTATTCGCATTATTTACCAGTCAGGCATGGAACATGGCGTTCTCGTTC
TACCAGTCATTACGTACTGTGCCACGCGATTTGCAAGAAGTCAGTCGTGGCTTTGGTTTCTCCGCTTGGTTGCGTTTTTG
GCGATTGGAGGTCCCGTTCGCGATGCCTGGACTCATCTGGAACATGATGATGTCCATGTCAGGGGGATGGTTCTTCGTGG
TGGCCTCCGAAGCGATTACGGTTGGTGATACCACAGTGACGCTTCCTGGTATTGGTTCCTACTTGGCGTTGGCGATCGCA
CATCAGCAGTTTGGTGCAGTTGCCTGGGCGGTGGCTGCGATGGCATTGCTCATCTTGCTTTACGACCAGCTGCTGTTCCG
CCCGATTGTGGTTTGGGCCGATAAGTTCCGGGTTGAGCTGACGGCTGGTCGTCAGATACCCAGATCCTGGGTTTACGATC
TGATTTGCCGTACCAGACTGCTAAAGCGCTTAGTTGCACCTCTCACTCGGCTTTGGCAGCGAGTGCTTATCCTACGTTGG
ACCAAGCGCAATAGAGTGACAGTGCTGCCTGTGTCGCCCCGGGCGGGTAGCGTGCGTGGAGATCGATTATGGCGGGTGGT
TTTGTTTGCTGTGGCTGCCGCTTGCATCTATTTTGTGTTCAATAGCCTATGGCAGACACTGGACGTAGAAGATCTTACAG
CTGCATTGGGTGGCGCTTTGGCGACATTACTCAGGGTGGTCGTATTGATTGCCTTGGCCTCGCTGATTTGGGTACCGATT
GGAGTGTGGATTGGATTGCGTCCGGTGCTCGCACAGCGGGTCCAGCCCTTGGTACAGTTTTTGGCTGCCTTCCCCGCTAA
CGTACTATTCCCGTTTGCGGTGGTCGCGATTGTGAGCACGGATGCGAATCCGAATATTTGGTTGTCGCCACTCATGGTGC
TGGGTACCCAGTGGTACATCCTGTTCAACGTGATTGCTGGAGCGAGCGCGTTTCCGACTGACCTGAAAGAGGCGGCCCAG
GTGTATCGCTTGCGTTCGTGGACCTGGTGGCGCCGGGTGATGTTACCTGGGATCTTCCCCTATTATCTGACCGGCGCGTT
GACGGCTTCAGGTGGCTCTTGGAACGCCAGTATCGTTGCCGAACTGGTCAGTTGGGGACACCAGCATCTGCAAGCCTATG
GCCTGGGGTCTTATATCGCCAGTGCGACGGAGGCCGGTGACTACCCCCGCGTACTCCTTGGAGTGGTGGTGATGTCGCTG
TTTGTCACCGTGTTTAACCGTACCTTGTGGCGTCGCTTATTTGTCTTTGCTGAGCGCCGTTTACGTTTGGATTGA

Upstream 100 bases:

>100_bases
GCTATTGCCTTTTCTTTGAGGTTTTATATCGGCTGCCGCGCTTTGGTTTGAATCTGTCTGCGTGCTGTTTTTTCCGTCCA
TGGAAATCAGGAGGTTCTGC

Downstream 100 bases:

>100_bases
CTCTTTTATCCTGCTTTTTATTTTTTCCACTGGAGTGCATCAATGAACCTGTCCGATGTCTCTCAGAAAAGCCCCTTGGT
GAGCGTGCGAGGGGTTTGTA

Product: ABC transporter nitrate permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MAFLSQNRPVHPAVASLVRAQLPNVYDLVALALILLLGIALLHGAEGASAPLSALPVEAVSLDPTHLPEYGLRTTLRMFA
AMTASLLFTFVVATLAAKSQRAERLIVPALDVLQSVPVLGFLTFTVTFFLGLFPGSQLGAECASVFALFTSQAWNMAFSF
YQSLRTVPRDLQEVSRGFGFSAWLRFWRLEVPFAMPGLIWNMMMSMSGGWFFVVASEAITVGDTTVTLPGIGSYLALAIA
HQQFGAVAWAVAAMALLILLYDQLLFRPIVVWADKFRVELTAGRQIPRSWVYDLICRTRLLKRLVAPLTRLWQRVLILRW
TKRNRVTVLPVSPRAGSVRGDRLWRVVLFAVAAACIYFVFNSLWQTLDVEDLTAALGGALATLLRVVVLIALASLIWVPI
GVWIGLRPVLAQRVQPLVQFLAAFPANVLFPFAVVAIVSTDANPNIWLSPLMVLGTQWYILFNVIAGASAFPTDLKEAAQ
VYRLRSWTWWRRVMLPGIFPYYLTGALTASGGSWNASIVAELVSWGHQHLQAYGLGSYIASATEAGDYPRVLLGVVVMSL
FVTVFNRTLWRRLFVFAERRLRLD

Sequences:

>Translated_584_residues
MAFLSQNRPVHPAVASLVRAQLPNVYDLVALALILLLGIALLHGAEGASAPLSALPVEAVSLDPTHLPEYGLRTTLRMFA
AMTASLLFTFVVATLAAKSQRAERLIVPALDVLQSVPVLGFLTFTVTFFLGLFPGSQLGAECASVFALFTSQAWNMAFSF
YQSLRTVPRDLQEVSRGFGFSAWLRFWRLEVPFAMPGLIWNMMMSMSGGWFFVVASEAITVGDTTVTLPGIGSYLALAIA
HQQFGAVAWAVAAMALLILLYDQLLFRPIVVWADKFRVELTAGRQIPRSWVYDLICRTRLLKRLVAPLTRLWQRVLILRW
TKRNRVTVLPVSPRAGSVRGDRLWRVVLFAVAAACIYFVFNSLWQTLDVEDLTAALGGALATLLRVVVLIALASLIWVPI
GVWIGLRPVLAQRVQPLVQFLAAFPANVLFPFAVVAIVSTDANPNIWLSPLMVLGTQWYILFNVIAGASAFPTDLKEAAQ
VYRLRSWTWWRRVMLPGIFPYYLTGALTASGGSWNASIVAELVSWGHQHLQAYGLGSYIASATEAGDYPRVLLGVVVMSL
FVTVFNRTLWRRLFVFAERRLRLD
>Mature_583_residues
AFLSQNRPVHPAVASLVRAQLPNVYDLVALALILLLGIALLHGAEGASAPLSALPVEAVSLDPTHLPEYGLRTTLRMFAA
MTASLLFTFVVATLAAKSQRAERLIVPALDVLQSVPVLGFLTFTVTFFLGLFPGSQLGAECASVFALFTSQAWNMAFSFY
QSLRTVPRDLQEVSRGFGFSAWLRFWRLEVPFAMPGLIWNMMMSMSGGWFFVVASEAITVGDTTVTLPGIGSYLALAIAH
QQFGAVAWAVAAMALLILLYDQLLFRPIVVWADKFRVELTAGRQIPRSWVYDLICRTRLLKRLVAPLTRLWQRVLILRWT
KRNRVTVLPVSPRAGSVRGDRLWRVVLFAVAAACIYFVFNSLWQTLDVEDLTAALGGALATLLRVVVLIALASLIWVPIG
VWIGLRPVLAQRVQPLVQFLAAFPANVLFPFAVVAIVSTDANPNIWLSPLMVLGTQWYILFNVIAGASAFPTDLKEAAQV
YRLRSWTWWRRVMLPGIFPYYLTGALTASGGSWNASIVAELVSWGHQHLQAYGLGSYIASATEAGDYPRVLLGVVVMSLF
VTVFNRTLWRRLFVFAERRLRLD

Specific function: Probably part of a high-affinity binding-protein- dependent transport system for nitrate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4986

COG function: function code P; ABC-type anion transport system, duplicated permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 64688; Mature: 64557

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLSQNRPVHPAVASLVRAQLPNVYDLVALALILLLGIALLHGAEGASAPLSALPVEAV
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHC
SLDPTHLPEYGLRTTLRMFAAMTASLLFTFVVATLAAKSQRAERLIVPALDVLQSVPVLG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
FLTFTVTFFLGLFPGSQLGAECASVFALFTSQAWNMAFSFYQSLRTVPRDLQEVSRGFGF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SAWLRFWRLEVPFAMPGLIWNMMMSMSGGWFFVVASEAITVGDTTVTLPGIGSYLALAIA
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEECCCEEECCCHHHHHHHHHH
HQQFGAVAWAVAAMALLILLYDQLLFRPIVVWADKFRVELTAGRQIPRSWVYDLICRTRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHH
LKRLVAPLTRLWQRVLILRWTKRNRVTVLPVSPRAGSVRGDRLWRVVLFAVAAACIYFVF
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NSLWQTLDVEDLTAALGGALATLLRVVVLIALASLIWVPIGVWIGLRPVLAQRVQPLVQF
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAFPANVLFPFAVVAIVSTDANPNIWLSPLMVLGTQWYILFNVIAGASAFPTDLKEAAQ
HHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VYRLRSWTWWRRVMLPGIFPYYLTGALTASGGSWNASIVAELVSWGHQHLQAYGLGSYIA
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SATEAGDYPRVLLGVVVMSLFVTVFNRTLWRRLFVFAERRLRLD
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AFLSQNRPVHPAVASLVRAQLPNVYDLVALALILLLGIALLHGAEGASAPLSALPVEAV
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHC
SLDPTHLPEYGLRTTLRMFAAMTASLLFTFVVATLAAKSQRAERLIVPALDVLQSVPVLG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
FLTFTVTFFLGLFPGSQLGAECASVFALFTSQAWNMAFSFYQSLRTVPRDLQEVSRGFGF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SAWLRFWRLEVPFAMPGLIWNMMMSMSGGWFFVVASEAITVGDTTVTLPGIGSYLALAIA
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEECCCEEECCCHHHHHHHHHH
HQQFGAVAWAVAAMALLILLYDQLLFRPIVVWADKFRVELTAGRQIPRSWVYDLICRTRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHH
LKRLVAPLTRLWQRVLILRWTKRNRVTVLPVSPRAGSVRGDRLWRVVLFAVAAACIYFVF
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NSLWQTLDVEDLTAALGGALATLLRVVVLIALASLIWVPIGVWIGLRPVLAQRVQPLVQF
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAFPANVLFPFAVVAIVSTDANPNIWLSPLMVLGTQWYILFNVIAGASAFPTDLKEAAQ
HHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VYRLRSWTWWRRVMLPGIFPYYLTGALTASGGSWNASIVAELVSWGHQHLQAYGLGSYIA
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SATEAGDYPRVLLGVVVMSLFVTVFNRTLWRRLFVFAERRLRLD
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7647306 [H]