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The map label for this gene is mscM [H]

Identifier: 28199518

GI number: 28199518

Start: 1900109

End: 1901527

Strand: Reverse

Name: mscM [H]

Synonym: PD1641

Alternate gene names: 28199518

Gene position: 1901527-1900109 (Counterclockwise)

Preceding gene: 28199519

Following gene: 28199517

Centisome position: 75.46

GC content: 49.82

Gene sequence:

>1419_bases
GTGGTGGTAAGACAAACGTGTGCTGCTGTTGAGATTATTTGTTTCTTGGTTCAATGCCAATGTGTGGGGATACGTGGGTA
CCCCATGCTGTACATTGAAGGGGGCTGTGATTTTGGAGTGATGATGTTCAATGCCAGCATTGCGATTACCTGGACCCATT
TGCATGATGTCTTGGCACCTTATCCTTGGGTCTACCGTGTATTAGCAACAATCATGTTGTTGTTGGCTGCTTGGTTTGCT
AATTGGGTGACGAAGCGGGTTTTGCTACGTGGTGTGCAGCGTGTGCTGCGTAGCAGTGTGCTTGCTGGCGAGGAGGGCGG
CTACGGCATGCGTTTGGGTGTCATCCCGCGCTTGGCTAATGTGGTTCCAGCATTGGTGTTGTCTCTGGGAGTGGCCGCGG
TGCCTGATGTGCCACTTGTGGTGGTTGCAGTGGTGCGCAATGTTTGTACGGCTTTTATCGTATTGACCGTGGCATTGGCG
TTGGCTCGTTTGATGGATTTGGCCAATCAAGTGTATGAGCGCCGTCCCGATGCGCAGCACAAGCCAATCAAGAGTTATGT
ACAGCTGCTGAAAATTGTGCTCGGTTTGTTTACGGCTGTACTGATGATTTCAGTATTGATAGACAAGTCGCCGCTGATCC
TTCTTTCTGGTCTGGGCGCCATGATGGCTGTGTTGATTTTGGTCTTTCAGGACACGTTGTTGTCGTTGGTCGCCAGTGTG
ACGATTAATTCCAACGATATGGTGCGGGTGGGTGATTGGATCGAAATGCCACAGCAGGGCGCTGATGGGGATGTGATTGA
TATTGCGCTGCATACGATCAAGGTCCAGAACTGGGACAAAACGATTACTGCAATTCCAATCAAGCGATTGATCAGTGATT
CGTTCAAGAACTGGCGTGGAATGCATGAGAGTGGTGGGCGCCGGATCAAGCGGTCGCTATTTCTGGATCAGTGCAGTGTA
CGTTTTCTTGATGATTCTGAAATTGCAAGGATGCGTGAGTTCAGCATATTGCGTGATTACATTGATGCAAAGTGCGAGAG
TTTGGCTGAGTGGAATGTGGCGCTGTCAAGTCAAGGTGTTGCTTCAGTCAATGCTCGCCGTATTACCAATCTTGGGACAT
TTCGCGCTTATGTGGAGCATTATCTGCGTGCCAGCCCGCATGTGCACCAGGAGATGACGTTGTTAGTACGGCAATTGCAA
CCAACGGATCTTGGGCTGCCTTTGGAGATCTACTGTTTTACAAATAACACGGATTGGGTGGCTTACGAGCAAATTCAATC
GGACATTTTCGATCATCTGTTGGCGTTGTTGCCGCACTTTGGGTTGAGGGTTTTTCAGAGTTCCAGTGACACCATGTTGA
TGGAAACGGCGCAACGTTTGAGTGCTATATCGGCTGCGTTAACGGGGCGTGCGCATTGA

Upstream 100 bases:

>100_bases
TTGGGAGGGTGCTGTTTCTTGGATCAATTTACGTGACTTAAGTCGCTATTTATGGGTGATATTCTCGTCTCCTTGTGTTG
GCTGCCACCTCAGATGGTCC

Downstream 100 bases:

>100_bases
CGTGTGTATGCGCACGACAGGCATTAAGAAGGATGTGTCCACGATAGAGCGACTTGATATGATGTTTTACGACGCACTTA
TGTTGTTTAGCAGTGTATTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MVVRQTCAAVEIICFLVQCQCVGIRGYPMLYIEGGCDFGVMMFNASIAITWTHLHDVLAPYPWVYRVLATIMLLLAAWFA
NWVTKRVLLRGVQRVLRSSVLAGEEGGYGMRLGVIPRLANVVPALVLSLGVAAVPDVPLVVVAVVRNVCTAFIVLTVALA
LARLMDLANQVYERRPDAQHKPIKSYVQLLKIVLGLFTAVLMISVLIDKSPLILLSGLGAMMAVLILVFQDTLLSLVASV
TINSNDMVRVGDWIEMPQQGADGDVIDIALHTIKVQNWDKTITAIPIKRLISDSFKNWRGMHESGGRRIKRSLFLDQCSV
RFLDDSEIARMREFSILRDYIDAKCESLAEWNVALSSQGVASVNARRITNLGTFRAYVEHYLRASPHVHQEMTLLVRQLQ
PTDLGLPLEIYCFTNNTDWVAYEQIQSDIFDHLLALLPHFGLRVFQSSSDTMLMETAQRLSAISAALTGRAH

Sequences:

>Translated_472_residues
MVVRQTCAAVEIICFLVQCQCVGIRGYPMLYIEGGCDFGVMMFNASIAITWTHLHDVLAPYPWVYRVLATIMLLLAAWFA
NWVTKRVLLRGVQRVLRSSVLAGEEGGYGMRLGVIPRLANVVPALVLSLGVAAVPDVPLVVVAVVRNVCTAFIVLTVALA
LARLMDLANQVYERRPDAQHKPIKSYVQLLKIVLGLFTAVLMISVLIDKSPLILLSGLGAMMAVLILVFQDTLLSLVASV
TINSNDMVRVGDWIEMPQQGADGDVIDIALHTIKVQNWDKTITAIPIKRLISDSFKNWRGMHESGGRRIKRSLFLDQCSV
RFLDDSEIARMREFSILRDYIDAKCESLAEWNVALSSQGVASVNARRITNLGTFRAYVEHYLRASPHVHQEMTLLVRQLQ
PTDLGLPLEIYCFTNNTDWVAYEQIQSDIFDHLLALLPHFGLRVFQSSSDTMLMETAQRLSAISAALTGRAH
>Mature_472_residues
MVVRQTCAAVEIICFLVQCQCVGIRGYPMLYIEGGCDFGVMMFNASIAITWTHLHDVLAPYPWVYRVLATIMLLLAAWFA
NWVTKRVLLRGVQRVLRSSVLAGEEGGYGMRLGVIPRLANVVPALVLSLGVAAVPDVPLVVVAVVRNVCTAFIVLTVALA
LARLMDLANQVYERRPDAQHKPIKSYVQLLKIVLGLFTAVLMISVLIDKSPLILLSGLGAMMAVLILVFQDTLLSLVASV
TINSNDMVRVGDWIEMPQQGADGDVIDIALHTIKVQNWDKTITAIPIKRLISDSFKNWRGMHESGGRRIKRSLFLDQCSV
RFLDDSEIARMREFSILRDYIDAKCESLAEWNVALSSQGVASVNARRITNLGTFRAYVEHYLRASPHVHQEMTLLVRQLQ
PTDLGLPLEIYCFTNNTDWVAYEQIQSDIFDHLLALLPHFGLRVFQSSSDTMLMETAQRLSAISAALTGRAH

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=298, Percent_Identity=50.3355704697987, Blast_Score=321, Evalue=8e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 52564; Mature: 52564

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVRQTCAAVEIICFLVQCQCVGIRGYPMLYIEGGCDFGVMMFNASIAITWTHLHDVLAP
CCCHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCEEEEEEECEEEEEHHHHHHHHCC
YPWVYRVLATIMLLLAAWFANWVTKRVLLRGVQRVLRSSVLAGEEGGYGMRLGVIPRLAN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
VVPALVLSLGVAAVPDVPLVVVAVVRNVCTAFIVLTVALALARLMDLANQVYERRPDAQH
HHHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KPIKSYVQLLKIVLGLFTAVLMISVLIDKSPLILLSGLGAMMAVLILVFQDTLLSLVASV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TINSNDMVRVGDWIEMPQQGADGDVIDIALHTIKVQNWDKTITAIPIKRLISDSFKNWRG
EECCCCCEEECHHHHCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
MHESGGRRIKRSLFLDQCSVRFLDDSEIARMREFSILRDYIDAKCESLAEWNVALSSQGV
CHHCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASVNARRITNLGTFRAYVEHYLRASPHVHQEMTLLVRQLQPTDLGLPLEIYCFTNNTDWV
HHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCH
AYEQIQSDIFDHLLALLPHFGLRVFQSSSDTMLMETAQRLSAISAALTGRAH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVVRQTCAAVEIICFLVQCQCVGIRGYPMLYIEGGCDFGVMMFNASIAITWTHLHDVLAP
CCCHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCEEEEEEECEEEEEHHHHHHHHCC
YPWVYRVLATIMLLLAAWFANWVTKRVLLRGVQRVLRSSVLAGEEGGYGMRLGVIPRLAN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
VVPALVLSLGVAAVPDVPLVVVAVVRNVCTAFIVLTVALALARLMDLANQVYERRPDAQH
HHHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KPIKSYVQLLKIVLGLFTAVLMISVLIDKSPLILLSGLGAMMAVLILVFQDTLLSLVASV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TINSNDMVRVGDWIEMPQQGADGDVIDIALHTIKVQNWDKTITAIPIKRLISDSFKNWRG
EECCCCCEEECHHHHCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
MHESGGRRIKRSLFLDQCSVRFLDDSEIARMREFSILRDYIDAKCESLAEWNVALSSQGV
CHHCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASVNARRITNLGTFRAYVEHYLRASPHVHQEMTLLVRQLQPTDLGLPLEIYCFTNNTDWV
HHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCH
AYEQIQSDIFDHLLALLPHFGLRVFQSSSDTMLMETAQRLSAISAALTGRAH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]