Click here to switch to the map view.

The map label for this gene is estA [H]

Identifier: 28199047

GI number: 28199047

Start: 1352538

End: 1353212

Strand: Reverse

Name: estA [H]

Synonym: PD1155

Alternate gene names: 28199047

Gene position: 1353212-1352538 (Counterclockwise)

Preceding gene: 77747680

Following gene: 28199046

Centisome position: 53.7

GC content: 52.15

Gene sequence:

>675_bases
ATGCTGGAAACGATAGAGCACGAGACTGCCCCTATGCCGCAGTGGAGTGTGTTGTGGTTACATGGTTTGGGGGCGGATGG
TCATGATTTCATGCCCATCATTCCAGAGTTGGTGCGTCCACACTGGCCAGCTTTGCGTTTTGTGTTTCCGCATGCTCCGG
TGAGGCCGATCACGATTAATAACGGTGTACCGATGCGTGCTTGGTACGATCTTGTCAGTTTTGATTTCAATCAACGTGCC
GACCAGACTGGGATTGAAGCTGCAGTTGCGCAGGTCCAGGCATTGATGATGCGCGAGCAGCAGCGTGGCATTGCGTCTGA
ACGCTTGTTTCTTGCCGGATTTTCGCAGGGAGGAGCGGTGGTGCTCAGTATCGGTTTGCGTTGTAAGGCCTACTTAGCAG
GCTTGATTGCATTGTCTACGTATCTACCGGATCCTAACGTTGTCACTACCACTACAGGGTTGCTTTCAGGCAGCAATGCG
CAACAGTTTTTTATTGCCCACGGACATTCCGATCCAGTGGTGCCACTGGTACATGGTCAGTGTGCTGCTGAGGTGTTGCG
TAAGCTAGGTTTCGCTGTGGAATGGTATACCTACACGATGGCGCATCAGGTTTGCCAAGAGGAGATCCAGGCCTTGGGAG
ACTGGTTAGAGCGCCGTTTTGCGATTTCTTCTTAA

Upstream 100 bases:

>100_bases
ATTCCAGAGGAAACAGCACCATCGAAGGTATTGATACGTGTCGCCGAACTTCGCCGCGCTAGGCAACCCTAATTTTTGAA
TCCCTCCTGTTTGGATATAT

Downstream 100 bases:

>100_bases
CTGAGCTGCTTTTGTGCCGCAATCATTGATGTTGCGTCCGTCTTGGTTGCCGGAGATGTGCCGGGTCCCACGCATTGTGG
TATTGCTAGGATTGGCTGAA

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MLETIEHETAPMPQWSVLWLHGLGADGHDFMPIIPELVRPHWPALRFVFPHAPVRPITINNGVPMRAWYDLVSFDFNQRA
DQTGIEAAVAQVQALMMREQQRGIASERLFLAGFSQGGAVVLSIGLRCKAYLAGLIALSTYLPDPNVVTTTTGLLSGSNA
QQFFIAHGHSDPVVPLVHGQCAAEVLRKLGFAVEWYTYTMAHQVCQEEIQALGDWLERRFAISS

Sequences:

>Translated_224_residues
MLETIEHETAPMPQWSVLWLHGLGADGHDFMPIIPELVRPHWPALRFVFPHAPVRPITINNGVPMRAWYDLVSFDFNQRA
DQTGIEAAVAQVQALMMREQQRGIASERLFLAGFSQGGAVVLSIGLRCKAYLAGLIALSTYLPDPNVVTTTTGLLSGSNA
QQFFIAHGHSDPVVPLVHGQCAAEVLRKLGFAVEWYTYTMAHQVCQEEIQALGDWLERRFAISS
>Mature_224_residues
MLETIEHETAPMPQWSVLWLHGLGADGHDFMPIIPELVRPHWPALRFVFPHAPVRPITINNGVPMRAWYDLVSFDFNQRA
DQTGIEAAVAQVQALMMREQQRGIASERLFLAGFSQGGAVVLSIGLRCKAYLAGLIALSTYLPDPNVVTTTTGLLSGSNA
QQFFIAHGHSDPVVPLVHGQCAAEVLRKLGFAVEWYTYTMAHQVCQEEIQALGDWLERRFAISS

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=207, Percent_Identity=35.2657004830918, Blast_Score=130, Evalue=8e-31,
Organism=Homo sapiens, GI9966764, Length=218, Percent_Identity=34.8623853211009, Blast_Score=124, Evalue=5e-29,
Organism=Homo sapiens, GI20270341, Length=206, Percent_Identity=23.7864077669903, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17508057, Length=209, Percent_Identity=33.0143540669856, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323147, Length=216, Percent_Identity=26.8518518518519, Blast_Score=89, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45553055, Length=204, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI45553057, Length=204, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI21357257, Length=204, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI161078179, Length=208, Percent_Identity=25.4807692307692, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24848; Mature: 24848

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLETIEHETAPMPQWSVLWLHGLGADGHDFMPIIPELVRPHWPALRFVFPHAPVRPITIN
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCEEEEC
NGVPMRAWYDLVSFDFNQRADQTGIEAAVAQVQALMMREQQRGIASERLFLAGFSQGGAV
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEECCCCCEE
VLSIGLRCKAYLAGLIALSTYLPDPNVVTTTTGLLSGSNAQQFFIAHGHSDPVVPLVHGQ
EEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCEEEECCH
CAAEVLRKLGFAVEWYTYTMAHQVCQEEIQALGDWLERRFAISS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLETIEHETAPMPQWSVLWLHGLGADGHDFMPIIPELVRPHWPALRFVFPHAPVRPITIN
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCEEEEC
NGVPMRAWYDLVSFDFNQRADQTGIEAAVAQVQALMMREQQRGIASERLFLAGFSQGGAV
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEECCCCCEE
VLSIGLRCKAYLAGLIALSTYLPDPNVVTTTTGLLSGSNAQQFFIAHGHSDPVVPLVHGQ
EEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCEEEECCH
CAAEVLRKLGFAVEWYTYTMAHQVCQEEIQALGDWLERRFAISS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA