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The map label for this gene is arrD [H]

Identifier: 28199005

GI number: 28199005

Start: 1308680

End: 1309174

Strand: Reverse

Name: arrD [H]

Synonym: PD1113

Alternate gene names: 28199005

Gene position: 1309174-1308680 (Counterclockwise)

Preceding gene: 28199006

Following gene: 28199004

Centisome position: 51.96

GC content: 54.75

Gene sequence:

>495_bases
ATGCAGACCATTGGTGAAGAAGGCATTGCACTGATTAAGTTTTTTGAGGGTTGCAAGCTGAGCTCGTATACCTGTCCTGG
GGGTGTGTTGACGATTGGCTATGGTGAGACGGGCAAGCACGTTACGCCTGATATGTGTCTTGCCAATGAGCAGGAAGCGG
ATGCGCGGTTACGTGCTCGATTGGCCAAGGAGTTTGAACCGGCGGTGCGGCGTTATGTGCGTGTGCCACTCAAGCAACAT
CAATTTGATGCGTTGGTATCGCTGAGCTTCAACATTGGTGCGGGCGCGTTCCACCGCTCGACGCTGTTACGCAAGCTTAA
TGCCGGTGATATTGCTGGTGCGGCGCAGCAGTTTCATGTGTGGAAATGGGCGGGCGGTCGTGTGCAGTCTGGCTTAATCA
TCAGGCGTGCCGCCGAACGTGCATTGTTTGAAGGCAGTGACTGGCGTGCTGAAGAGAAGCAACGGCGTGCCGCTTTAAAG
GGTCGCCGTGATTGA

Upstream 100 bases:

>100_bases
CTGCCGGTATTTCATCGCACCCGCCACGGAAAACCTGTCACAGGACGTATGCCCAGGATACACGCAGGATGAGCGGCAAT
GAATGTCTCCGGGACGTGTC

Downstream 100 bases:

>100_bases
TCCCTCGGCCATGATGTCCTGGTGGAAAGAAGCGTTCTACACGTGCCTTGCGATGGTGGCTGGGGTACTCGGCTACTTAA
TGCGCACGCTCGACAACGGC

Product: phage-related lysozyme

Products: NA

Alternate protein names: Endolysin; Lysis protein; Muramidase [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MQTIGEEGIALIKFFEGCKLSSYTCPGGVLTIGYGETGKHVTPDMCLANEQEADARLRARLAKEFEPAVRRYVRVPLKQH
QFDALVSLSFNIGAGAFHRSTLLRKLNAGDIAGAAQQFHVWKWAGGRVQSGLIIRRAAERALFEGSDWRAEEKQRRAALK
GRRD

Sequences:

>Translated_164_residues
MQTIGEEGIALIKFFEGCKLSSYTCPGGVLTIGYGETGKHVTPDMCLANEQEADARLRARLAKEFEPAVRRYVRVPLKQH
QFDALVSLSFNIGAGAFHRSTLLRKLNAGDIAGAAQQFHVWKWAGGRVQSGLIIRRAAERALFEGSDWRAEEKQRRAALK
GRRD
>Mature_164_residues
MQTIGEEGIALIKFFEGCKLSSYTCPGGVLTIGYGETGKHVTPDMCLANEQEADARLRARLAKEFEPAVRRYVRVPLKQH
QFDALVSLSFNIGAGAFHRSTLLRKLNAGDIAGAAQQFHVWKWAGGRVQSGLIIRRAAERALFEGSDWRAEEKQRRAALK
GRRD

Specific function: Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity [H]

COG id: COG3772

COG function: function code R; Phage-related lysozyme (muraminidase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 24 family [H]

Homologues:

Organism=Escherichia coli, GI1786768, Length=131, Percent_Identity=36.6412213740458, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002196 [H]

Pfam domain/function: PF00959 Phage_lysozyme [H]

EC number: =3.2.1.17 [H]

Molecular weight: Translated: 18243; Mature: 18243

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTIGEEGIALIKFFEGCKLSSYTCPGGVLTIGYGETGKHVTPDMCLANEQEADARLRAR
CCCCCCHHHHHHHHHCCCCCCCEECCCCEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHH
LAKEFEPAVRRYVRVPLKQHQFDALVSLSFNIGAGAFHRSTLLRKLNAGDIAGAAQQFHV
HHHHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCHHCCHHHEEE
WKWAGGRVQSGLIIRRAAERALFEGSDWRAEEKQRRAALKGRRD
EECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQTIGEEGIALIKFFEGCKLSSYTCPGGVLTIGYGETGKHVTPDMCLANEQEADARLRAR
CCCCCCHHHHHHHHHCCCCCCCEECCCCEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHH
LAKEFEPAVRRYVRVPLKQHQFDALVSLSFNIGAGAFHRSTLLRKLNAGDIAGAAQQFHV
HHHHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCHHCCHHHEEE
WKWAGGRVQSGLIIRRAAERALFEGSDWRAEEKQRRAALKGRRD
EECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]