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The map label for this gene is oprB [H]

Identifier: 262192860

GI number: 262192860

Start: 1172153

End: 1173691

Strand: Direct

Name: oprB [H]

Synonym: BMA10247_A1203

Alternate gene names: 262192860

Gene position: 1172153-1173691 (Clockwise)

Preceding gene: 126447020

Following gene: 126446480

Centisome position: 49.82

GC content: 71.47

Gene sequence:

>1539_bases
GTGCGCACGGCGGTTCGCGCCGCCGCCGTCGCGGCCGCCTTCTTCGCCGCGGGCTGCACGCTCGCGCCGCGCTACGAGCG
TCCGGCCGCGCCGGTGTCGGGCGCGTTCCCCGCCGACGGCGTCTATGCCGCGCAGCCGGGCGCCGCGCCCGGCGCGCGCA
GCGCGAACGGCCAGGCGGCCGTCGATATCGGCTGGCGCGAGTTCTTCGTCGATCCGCGCCTGCAGCGGCTGATCGAGATC
GCGCTGAAGAACAACCGCGACCTGCGCGTGTCGGTGCTCAACGTCGAGGCGTCGCGCGCGCAGTATCAGATCACGCGCGC
GGGGCTCTTCCCGACGTTGAGCGGCACCGGCACGGGCACGATCCAGCGCACGCCGGCCGGCGTGTCGATCACCGGCCAGC
CGCTCATCTCGCGGACCTACAACGTCGGCGTCTCCGCGTCGTGGGAGCTCGACCTGTTCGGCCGCGTGCAGAGCCTGAAG
GACCAGGCGCTCGCGCAATACTTCGCCACCGCGCAGGCGCGCAAGGCCGCGGAGATCTCGCTCGTCGCGAGCGTCGCCGA
TCAGTACCTGACGCTGCTGTCGACCGACGATCTGCTGCAGGTCACGCAGAACACGCTGAAGTCGGCGCGCGCGTCCTACG
ATCTGACGAAGCTGCAGTTCGACAACGGCACCGGCTCGGAGCTCGACCTGCGCCAGGCGCAGACGGTGGTCGAGACCGCG
CTCGCGAGCCAGCAGGCGCAGGCGCGCGCCCGCGCGCAGGCGCTCAACGCGCTCGTGCTGCTGATCGGCGAGCCGCTGCC
CGACGATCTGCCGGCCGACCTGCCACTCAATGCGCAGAACCTGCTCACCGACATTCCGGCCGGGCTGCCGTCCGATCTGC
TCACGCGGCGGCCCGACATCATGCAAGCCGAGGAGACGCTGCGCGCGGCGAACGCGAACATCGGCGCGGCGCGCGCGGCG
TTCTTCCCGAAGATCTCGCTCACCAGCGCGTTCGGCACCGCGAGCACGACGCTCGGCGGCCTGTTCAAGGCGGGCACGGC
GGCGTGGTCGTTCGCGCCGAACATCGCGCTGCCGATCTTCGAGGGCGGGCAGAACATCGCGAACCTCGATCTCGCGCACG
TGCAGAAGCGCATCGAGATCGCGAACTACGAGAAGGCGATCCAGAGCGCGTTTCGCGAGGTGTCGGACGGGCTTGCCGCG
CGCGGCACGTACGATCAGCAGATCGCGGCGCTCGAGCGCAACGAGCACGCGCAGCAGCGCCGCTACGATCTGTCGGACCT
GCGCTACAGGAACGGCGTCGACAGCTATCTGTCGGTGCTGACCGCGCAGACGGACCTGTATTCGGCGCAGCAGCAGTTGA
TCAGCGCGCGGCTCGCGCGCTGGACGAACCTCGTGGACCTGTATCGCGCGCTGGGCGGCGGGTGGCTCGAGCGCGCGGGC
GAGACGCCGCGCCCGGCGGACGCGCCCGTCGATTACGGCAAGGCGGCGCCCGCGCCGGCGGCGTCGGCATCGGCGCGAAC
AGCGGCCGTGATTGGTTGA

Upstream 100 bases:

>100_bases
TACGGCGGCGGCCTCGCGCCGTTGCCGCCGCCGCGCGGGCGGCGCCGCGCGCGCTTCGTGCGCTTCGTGCGCCGCTTCGC
GCGTCGCGTGCGCGTCGCTC

Downstream 100 bases:

>100_bases
GCGCGCAACCCGACATGCGGCAACCCAACGCGCGGCAGCACAGCGCGCGGCGCCCGAGCGCAGCGCGCGCCGGCCGAGAG
CGAATCGGGCGCACCTGGCG

Product: outer membrane efflux protein OprB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MRTAVRAAAVAAAFFAAGCTLAPRYERPAAPVSGAFPADGVYAAQPGAAPGARSANGQAAVDIGWREFFVDPRLQRLIEI
ALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGTIQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLK
DQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQVTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETA
LASQQAQARARAQALNALVLLIGEPLPDDLPADLPLNAQNLLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAA
FFPKISLTSAFGTASTTLGGLFKAGTAAWSFAPNIALPIFEGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAA
RGTYDQQIAALERNEHAQQRRYDLSDLRYRNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAG
ETPRPADAPVDYGKAAPAPAASASARTAAVIG

Sequences:

>Translated_512_residues
MRTAVRAAAVAAAFFAAGCTLAPRYERPAAPVSGAFPADGVYAAQPGAAPGARSANGQAAVDIGWREFFVDPRLQRLIEI
ALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGTIQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLK
DQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQVTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETA
LASQQAQARARAQALNALVLLIGEPLPDDLPADLPLNAQNLLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAA
FFPKISLTSAFGTASTTLGGLFKAGTAAWSFAPNIALPIFEGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAA
RGTYDQQIAALERNEHAQQRRYDLSDLRYRNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAG
ETPRPADAPVDYGKAAPAPAASASARTAAVIG
>Mature_512_residues
MRTAVRAAAVAAAFFAAGCTLAPRYERPAAPVSGAFPADGVYAAQPGAAPGARSANGQAAVDIGWREFFVDPRLQRLIEI
ALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGTIQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLK
DQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQVTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETA
LASQQAQARARAQALNALVLLIGEPLPDDLPADLPLNAQNLLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAA
FFPKISLTSAFGTASTTLGGLFKAGTAAWSFAPNIALPIFEGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAA
RGTYDQQIAALERNEHAQQRRYDLSDLRYRNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAG
ETPRPADAPVDYGKAAPAPAASASARTAAVIG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=458, Percent_Identity=41.703056768559, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI2367348, Length=425, Percent_Identity=24.7058823529412, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87082199, Length=315, Percent_Identity=23.8095238095238, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54510; Mature: 54510

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTAVRAAAVAAAFFAAGCTLAPRYERPAAPVSGAFPADGVYAAQPGAAPGARSANGQAA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEE
VDIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGT
EECCHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHHEEEEHHCCCCCCCCCCCCE
IQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLKDQALAQYFATAQARKAAEIS
EEECCCCCEEECCHHHHHHEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVASVADQYLTLLSTDDLLQVTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LASQQAQARARAQALNALVLLIGEPLPDDLPADLPLNAQNLLTDIPAGLPSDLLTRRPDI
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCH
MQAEETLRAANANIGAARAAFFPKISLTSAFGTASTTLGGLFKAGTAAWSFAPNIALPIF
HHHHHHHHHHCCCCCHHHHHHCCCEEHHHHCCCHHHHHHHHHHHCCHHEECCCCCEEEEE
EGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQR
CCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHH
RYDLSDLRYRNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAG
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
ETPRPADAPVDYGKAAPAPAASASARTAAVIG
CCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MRTAVRAAAVAAAFFAAGCTLAPRYERPAAPVSGAFPADGVYAAQPGAAPGARSANGQAA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEE
VDIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGT
EECCHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHHEEEEHHCCCCCCCCCCCCE
IQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLKDQALAQYFATAQARKAAEIS
EEECCCCCEEECCHHHHHHEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVASVADQYLTLLSTDDLLQVTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LASQQAQARARAQALNALVLLIGEPLPDDLPADLPLNAQNLLTDIPAGLPSDLLTRRPDI
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCH
MQAEETLRAANANIGAARAAFFPKISLTSAFGTASTTLGGLFKAGTAAWSFAPNIALPIF
HHHHHHHHHHCCCCCHHHHHHCCCEEHHHHCCCHHHHHHHHHHHCCHHEECCCCCEEEEE
EGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQR
CCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHH
RYDLSDLRYRNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAG
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
ETPRPADAPVDYGKAAPAPAASASARTAAVIG
CCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]