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The map label for this gene is tgt [H]

Identifier: 239618056

GI number: 239618056

Start: 1817338

End: 1818471

Strand: Reverse

Name: tgt [H]

Synonym: Kole_1685

Alternate gene names: 239618056

Gene position: 1818471-1817338 (Counterclockwise)

Preceding gene: 239618057

Following gene: 239618055

Centisome position: 78.99

GC content: 45.41

Gene sequence:

>1134_bases
ATGGTTAAATTCACCGTCACAAAAAAAGACCCGGTAACATCCGCGAGAACTGGAAAGCTAGAAACAAAACACGGTGTTTT
CGAAACACCTGTGTTTATGCCTGTGGGTACCAACGCTACCGTCAAAGGACTCTGGCAGGAGCAATTAAAGGAGCTTGGGG
CAAGAATCATTCTTGGCAATGCTTTCCACCTTTATTTAAAGCCTGGTCTCGAGGTAATAAGAAGCTTTGGCGGTCTGCAC
AGTTTCATGAACTGGAATAATTCTATACTCACCGACAGTGGAGGATTTCAGGTTTTCAGCCTTAAAGATAAAAAAGTTCT
GGACGAAGGTGTTCTGTTTAAATCGCCCATCGATGGCTCCAAGATCATGATGACACCGGAATTATCCATGGAAATACAGA
TGACGCTCGGATCGGATATAGCGATGGCATTCGACGAATGTGTCGCGCCTGAGTTAGACAGAACCTACGCACAAGAATCG
GTGGAAAGGACAACCAGATGGGCAGAAAAGTGCTTAGCGTATCATGACCGTTCTCAGGCGCTCTTTGGAATCGTTCAGGG
TGCTTTTTACAAAGACCTGCGCGAAAAGAGCGCCAGGGAAATTACAGCCATGGATTTCGATGGATTCGCCGTAGGTGGTT
TGAGTGTTGGTGAACCCTTTGAGGTGACCAGGGATATTCTCGAGTTTACAACAAAACTCCTTCCGGAGGATAAGCCAAGA
TATCTCATGGGAATTGGTACGCCAGATATGATAATCATGGCTGTCGAGAACGGCATCGATATGTTCGACTGCGTCCTTCC
AACGCGAATGGGAAGGCATGGAACAGCATTAACATGGTCCGGAAAAATCAATCTCAAAGCTGCCAGGCACAAGTTTTCCA
AAGAACCCATCGATCCTGAGTGCAACTGTAAGGTTTGCAGAAGCTACTCAAAAGGTTACATATATCACCTGTTTTCACGT
GATGAGCTCCTTGGAAAAATGATGCTGAGTTACCATAATGTGCATTTTTTGCTGGATTTCACCGACCGACTCAGAAATGC
CATTCGCGAAGAAAGATTCTTAGAGTTCAAAGAGTTGTGTACCCGAAAACAGTTAATCAAGAACGATACCGGGGTAAATG
TATCCGAGGGGTGA

Upstream 100 bases:

>100_bases
GATCCACGGTACTGTAACTGGATCAAAGAAGAAATGGACCATCTCGAAAAGCAAATCGAAGCCTCCTACCGGAGGATACT
CGAGGTGATAAAAAAGAGAA

Downstream 100 bases:

>100_bases
TTGAATGAAAAAATATTATCTCATAATTCTTCTGGGTCTATTATCGGTTACAGGTTTTTCCTTTGACCCGTTCCTTGTTC
AGACTGGCGGAAATTCAATT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MVKFTVTKKDPVTSARTGKLETKHGVFETPVFMPVGTNATVKGLWQEQLKELGARIILGNAFHLYLKPGLEVIRSFGGLH
SFMNWNNSILTDSGGFQVFSLKDKKVLDEGVLFKSPIDGSKIMMTPELSMEIQMTLGSDIAMAFDECVAPELDRTYAQES
VERTTRWAEKCLAYHDRSQALFGIVQGAFYKDLREKSAREITAMDFDGFAVGGLSVGEPFEVTRDILEFTTKLLPEDKPR
YLMGIGTPDMIIMAVENGIDMFDCVLPTRMGRHGTALTWSGKINLKAARHKFSKEPIDPECNCKVCRSYSKGYIYHLFSR
DELLGKMMLSYHNVHFLLDFTDRLRNAIREERFLEFKELCTRKQLIKNDTGVNVSEG

Sequences:

>Translated_377_residues
MVKFTVTKKDPVTSARTGKLETKHGVFETPVFMPVGTNATVKGLWQEQLKELGARIILGNAFHLYLKPGLEVIRSFGGLH
SFMNWNNSILTDSGGFQVFSLKDKKVLDEGVLFKSPIDGSKIMMTPELSMEIQMTLGSDIAMAFDECVAPELDRTYAQES
VERTTRWAEKCLAYHDRSQALFGIVQGAFYKDLREKSAREITAMDFDGFAVGGLSVGEPFEVTRDILEFTTKLLPEDKPR
YLMGIGTPDMIIMAVENGIDMFDCVLPTRMGRHGTALTWSGKINLKAARHKFSKEPIDPECNCKVCRSYSKGYIYHLFSR
DELLGKMMLSYHNVHFLLDFTDRLRNAIREERFLEFKELCTRKQLIKNDTGVNVSEG
>Mature_377_residues
MVKFTVTKKDPVTSARTGKLETKHGVFETPVFMPVGTNATVKGLWQEQLKELGARIILGNAFHLYLKPGLEVIRSFGGLH
SFMNWNNSILTDSGGFQVFSLKDKKVLDEGVLFKSPIDGSKIMMTPELSMEIQMTLGSDIAMAFDECVAPELDRTYAQES
VERTTRWAEKCLAYHDRSQALFGIVQGAFYKDLREKSAREITAMDFDGFAVGGLSVGEPFEVTRDILEFTTKLLPEDKPR
YLMGIGTPDMIIMAVENGIDMFDCVLPTRMGRHGTALTWSGKINLKAARHKFSKEPIDPECNCKVCRSYSKGYIYHLFSR
DELLGKMMLSYHNVHFLLDFTDRLRNAIREERFLEFKELCTRKQLIKNDTGVNVSEG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=349, Percent_Identity=44.6991404011461, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI13375872, Length=255, Percent_Identity=26.2745098039216, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1786607, Length=346, Percent_Identity=48.2658959537572, Blast_Score=343, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17544680, Length=345, Percent_Identity=41.1594202898551, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI25146502, Length=191, Percent_Identity=26.7015706806283, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19920510, Length=358, Percent_Identity=44.1340782122905, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI21355311, Length=280, Percent_Identity=23.5714285714286, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42662; Mature: 42662

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKFTVTKKDPVTSARTGKLETKHGVFETPVFMPVGTNATVKGLWQEQLKELGARIILGN
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECC
AFHLYLKPGLEVIRSFGGLHSFMNWNNSILTDSGGFQVFSLKDKKVLDEGVLFKSPIDGS
EEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCHHHHHCCCEEECCCCCC
KIMMTPELSMEIQMTLGSDIAMAFDECVAPELDRTYAQESVERTTRWAEKCLAYHDRSQA
EEEECCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LFGIVQGAFYKDLREKSAREITAMDFDGFAVGGLSVGEPFEVTRDILEFTTKLLPEDKPR
HHHHHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
YLMGIGTPDMIIMAVENGIDMFDCVLPTRMGRHGTALTWSGKINLKAARHKFSKEPIDPE
EEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEECCEEEEHHHHHHHCCCCCCCC
CNCKVCRSYSKGYIYHLFSRDELLGKMMLSYHNVHFLLDFTDRLRNAIREERFLEFKELC
CCHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
TRKQLIKNDTGVNVSEG
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVKFTVTKKDPVTSARTGKLETKHGVFETPVFMPVGTNATVKGLWQEQLKELGARIILGN
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECC
AFHLYLKPGLEVIRSFGGLHSFMNWNNSILTDSGGFQVFSLKDKKVLDEGVLFKSPIDGS
EEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCHHHHHCCCEEECCCCCC
KIMMTPELSMEIQMTLGSDIAMAFDECVAPELDRTYAQESVERTTRWAEKCLAYHDRSQA
EEEECCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LFGIVQGAFYKDLREKSAREITAMDFDGFAVGGLSVGEPFEVTRDILEFTTKLLPEDKPR
HHHHHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
YLMGIGTPDMIIMAVENGIDMFDCVLPTRMGRHGTALTWSGKINLKAARHKFSKEPIDPE
EEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEECCEEEEHHHHHHHCCCCCCCC
CNCKVCRSYSKGYIYHLFSRDELLGKMMLSYHNVHFLLDFTDRLRNAIREERFLEFKELC
CCHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
TRKQLIKNDTGVNVSEG
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA