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The map label for this gene is appF [H]

Identifier: 239617918

GI number: 239617918

Start: 1657454

End: 1658659

Strand: Reverse

Name: appF [H]

Synonym: Kole_1546

Alternate gene names: 239617918

Gene position: 1658659-1657454 (Counterclockwise)

Preceding gene: 239617919

Following gene: 239617914

Centisome position: 72.05

GC content: 45.94

Gene sequence:

>1206_bases
ATGAATAACCAGACAAACCCAAAAAACACCCCCAAACCGTTGCTCAGGATAGATAATCTAGTCAAATATTTCCCGGTAAA
AGCGGGTGTTTTCCGACGTGTGGTTGCTCAGGTTAAGGCTGTTGATGATGTTAGTTTCGAGGTTTATGAACGGGAAACCC
TCGGGCTCGTCGGCGAATCTGGATGTGGTAAGACGACAGCGGGTATGACCGTTCTAAGGCTCTACGAACCCACATCCGGA
AGAATTATCCTGAACGATGAGGATACTACCCATTATTTTCTTCCGATAATGAAAGCAAGGGCATATCTCAAACGCATGTA
TGTGGAACGTTTTCTTGACCTTAAAAACGCCGCGGGTTCAGTTGAGTCCGCTATCAAGAGTATTGAAGACGACTTTGATC
GCAAGATGGCTAAACTTTTTTTCGAGGTTCACAACGGATCCGCTTCGGCCTTCATTCGTGACATGATGAGTGCCAGGGAA
GCGAAACGAAAAAAATTCAGACGCCAGGCCCAGATGATCTTTCAGGATCCATTTTCTTCACTCAATCCCAGGATGAGAGT
CAAGAACATCATAGCCGAAGGCGCTCTCATTCACCACCTTGCGACACGCTCAGAAGTCATGGACAAAGTTGCCGATATAT
TGAAAAAAGTAGGGCTTTCAGCAGATCATATGGGAAGATTCCCTCACGAATTCAGCGGAGGACAAAGGCAAAGAATAGGT
ATTGCTAGAGCCTTGATACTCAATCCGAAGCTCATCGTAGCAGACGAGGCCGTTTCCGCTCTTGACGTCTCAATACAGGC
TCAGATATTGAATCTGCTTAATGATTTGCAGAAAGAATTTGGTTTGACGTATCTGTTCATCGCCCACGATCTCGCTGTTG
TTAAACATGTCAGTAAAAGGGTTGCTGTTATGTACCTTGGAAAGATCGTCGAGATCGCCGACAAAAAAGAGCTCTTTGAA
AATCCTTTGCACCCTTACACTGTAGCATTGATGTCAGCAATACCGATCCCCGATCCAGAGAAAAAGAGCAAGAGAATCAT
TCTCAAAGGCGATGTCCCCAGTCCTATAAATCCACCCAGTGGCTGCCGTTTCCACCCACGCTGTCCAATAGCAAAAGAAG
TTTGTTCGAAGGAAGAACCGCCATTGAAGGATGTCGGAAACGGCCACAAGGTCGCCTGTTTCTTCCCTGGAGAACTGAAA
AGATAA

Upstream 100 bases:

>100_bases
AACCACCACTGGAAGAGATTGAATCCAGTCACAAAGTAGCTTGCTGGCACGTAGATAAACTCGAAGAAATGATCAAAGTG
GCAAGGGCAGGTGAAGGTGC

Downstream 100 bases:

>100_bases
TAGAAGTCTGGTTTTCTCTAGAGCATTCCGCCTATACCATCGACGACAAGGTCGATCTGGGCGGAATGTTTTTTTATCAA
TTCCCTGAGTTCCTTCATTG

Product: oligopeptide/dipeptide ABC transporter, ATPase subunit

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNNQTNPKNTPKPLLRIDNLVKYFPVKAGVFRRVVAQVKAVDDVSFEVYERETLGLVGESGCGKTTAGMTVLRLYEPTSG
RIILNDEDTTHYFLPIMKARAYLKRMYVERFLDLKNAAGSVESAIKSIEDDFDRKMAKLFFEVHNGSASAFIRDMMSARE
AKRKKFRRQAQMIFQDPFSSLNPRMRVKNIIAEGALIHHLATRSEVMDKVADILKKVGLSADHMGRFPHEFSGGQRQRIG
IARALILNPKLIVADEAVSALDVSIQAQILNLLNDLQKEFGLTYLFIAHDLAVVKHVSKRVAVMYLGKIVEIADKKELFE
NPLHPYTVALMSAIPIPDPEKKSKRIILKGDVPSPINPPSGCRFHPRCPIAKEVCSKEEPPLKDVGNGHKVACFFPGELK
R

Sequences:

>Translated_401_residues
MNNQTNPKNTPKPLLRIDNLVKYFPVKAGVFRRVVAQVKAVDDVSFEVYERETLGLVGESGCGKTTAGMTVLRLYEPTSG
RIILNDEDTTHYFLPIMKARAYLKRMYVERFLDLKNAAGSVESAIKSIEDDFDRKMAKLFFEVHNGSASAFIRDMMSARE
AKRKKFRRQAQMIFQDPFSSLNPRMRVKNIIAEGALIHHLATRSEVMDKVADILKKVGLSADHMGRFPHEFSGGQRQRIG
IARALILNPKLIVADEAVSALDVSIQAQILNLLNDLQKEFGLTYLFIAHDLAVVKHVSKRVAVMYLGKIVEIADKKELFE
NPLHPYTVALMSAIPIPDPEKKSKRIILKGDVPSPINPPSGCRFHPRCPIAKEVCSKEEPPLKDVGNGHKVACFFPGELK
R
>Mature_401_residues
MNNQTNPKNTPKPLLRIDNLVKYFPVKAGVFRRVVAQVKAVDDVSFEVYERETLGLVGESGCGKTTAGMTVLRLYEPTSG
RIILNDEDTTHYFLPIMKARAYLKRMYVERFLDLKNAAGSVESAIKSIEDDFDRKMAKLFFEVHNGSASAFIRDMMSARE
AKRKKFRRQAQMIFQDPFSSLNPRMRVKNIIAEGALIHHLATRSEVMDKVADILKKVGLSADHMGRFPHEFSGGQRQRIG
IARALILNPKLIVADEAVSALDVSIQAQILNLLNDLQKEFGLTYLFIAHDLAVVKHVSKRVAVMYLGKIVEIADKKELFE
NPLHPYTVALMSAIPIPDPEKKSKRIILKGDVPSPINPPSGCRFHPRCPIAKEVCSKEEPPLKDVGNGHKVACFFPGELK
R

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG4608

COG function: function code E; ABC-type oligopeptide transport system, ATPase component

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI171184400, Length=130, Percent_Identity=37.6923076923077, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI42741659, Length=139, Percent_Identity=35.2517985611511, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787500, Length=293, Percent_Identity=50.5119453924915, Blast_Score=277, Evalue=9e-76,
Organism=Escherichia coli, GI1789962, Length=385, Percent_Identity=38.1818181818182, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI145693107, Length=233, Percent_Identity=51.5021459227468, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI48994883, Length=256, Percent_Identity=41.40625, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1787758, Length=232, Percent_Identity=43.9655172413793, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1789963, Length=386, Percent_Identity=33.160621761658, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1787759, Length=265, Percent_Identity=35.8490566037736, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788506, Length=190, Percent_Identity=42.6315789473684, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1787548, Length=261, Percent_Identity=36.7816091954023, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787547, Length=169, Percent_Identity=38.4615384615385, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789891, Length=153, Percent_Identity=45.7516339869281, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786398, Length=202, Percent_Identity=36.1386138613861, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI48994997, Length=254, Percent_Identity=27.1653543307087, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789032, Length=178, Percent_Identity=35.3932584269663, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787712, Length=173, Percent_Identity=31.2138728323699, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787370, Length=167, Percent_Identity=32.9341317365269, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788225, Length=172, Percent_Identity=33.1395348837209, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788644, Length=187, Percent_Identity=31.0160427807487, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87081791, Length=200, Percent_Identity=30, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786872, Length=115, Percent_Identity=40, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787029, Length=157, Percent_Identity=33.7579617834395, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081709, Length=166, Percent_Identity=32.5301204819277, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789890, Length=169, Percent_Identity=36.094674556213, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789672, Length=171, Percent_Identity=31.5789473684211, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787089, Length=167, Percent_Identity=30.5389221556886, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790162, Length=161, Percent_Identity=29.1925465838509, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082267, Length=168, Percent_Identity=30.3571428571429, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788761, Length=127, Percent_Identity=31.496062992126, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081834, Length=99, Percent_Identity=40.4040404040404, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788450, Length=154, Percent_Identity=24.025974025974, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790467, Length=166, Percent_Identity=25.3012048192771, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786563, Length=120, Percent_Identity=37.5, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789873, Length=144, Percent_Identity=34.7222222222222, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787164, Length=93, Percent_Identity=38.7096774193548, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786253, Length=155, Percent_Identity=29.0322580645161, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17558664, Length=131, Percent_Identity=36.6412213740458, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193209708, Length=103, Percent_Identity=44.6601941747573, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17565938, Length=131, Percent_Identity=38.1679389312977, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=186, Percent_Identity=28.494623655914, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71996809, Length=186, Percent_Identity=28.494623655914, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193211017, Length=186, Percent_Identity=28.494623655914, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193202349, Length=178, Percent_Identity=29.7752808988764, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25146777, Length=158, Percent_Identity=32.9113924050633, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543740, Length=92, Percent_Identity=43.4782608695652, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193208177, Length=195, Percent_Identity=27.6923076923077, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI212646699, Length=169, Percent_Identity=30.1775147928994, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115534520, Length=80, Percent_Identity=46.25, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115535101, Length=113, Percent_Identity=37.1681415929204, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17567269, Length=172, Percent_Identity=30.2325581395349, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17541710, Length=89, Percent_Identity=39.3258426966292, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324985, Length=233, Percent_Identity=26.1802575107296, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323217, Length=95, Percent_Identity=38.9473684210526, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641565, Length=99, Percent_Identity=39.3939393939394, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI23397593, Length=119, Percent_Identity=38.655462184874, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85726357, Length=200, Percent_Identity=31.5, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17136662, Length=123, Percent_Identity=34.1463414634146, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593
- InterPro:   IPR010066
- InterPro:   IPR013563 [H]

Pfam domain/function: PF00005 ABC_tran; PF08352 oligo_HPY [H]

EC number: NA

Molecular weight: Translated: 45034; Mature: 45034

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQTNPKNTPKPLLRIDNLVKYFPVKAGVFRRVVAQVKAVDDVSFEVYERETLGLVGES
CCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
GCGKTTAGMTVLRLYEPTSGRIILNDEDTTHYFLPIMKARAYLKRMYVERFLDLKNAAGS
CCCCCHHCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VESAIKSIEDDFDRKMAKLFFEVHNGSASAFIRDMMSAREAKRKKFRRQAQMIFQDPFSS
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LNPRMRVKNIIAEGALIHHLATRSEVMDKVADILKKVGLSADHMGRFPHEFSGGQRQRIG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHH
IARALILNPKLIVADEAVSALDVSIQAQILNLLNDLQKEFGLTYLFIAHDLAVVKHVSKR
HHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHH
VAVMYLGKIVEIADKKELFENPLHPYTVALMSAIPIPDPEKKSKRIILKGDVPSPINPPS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCC
GCRFHPRCPIAKEVCSKEEPPLKDVGNGHKVACFFPGELKR
CCCCCCCCCHHHHHHCCCCCCHHHCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MNNQTNPKNTPKPLLRIDNLVKYFPVKAGVFRRVVAQVKAVDDVSFEVYERETLGLVGES
CCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
GCGKTTAGMTVLRLYEPTSGRIILNDEDTTHYFLPIMKARAYLKRMYVERFLDLKNAAGS
CCCCCHHCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VESAIKSIEDDFDRKMAKLFFEVHNGSASAFIRDMMSAREAKRKKFRRQAQMIFQDPFSS
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LNPRMRVKNIIAEGALIHHLATRSEVMDKVADILKKVGLSADHMGRFPHEFSGGQRQRIG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHH
IARALILNPKLIVADEAVSALDVSIQAQILNLLNDLQKEFGLTYLFIAHDLAVVKHVSKR
HHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHH
VAVMYLGKIVEIADKKELFENPLHPYTVALMSAIPIPDPEKKSKRIILKGDVPSPINPPS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCC
GCRFHPRCPIAKEVCSKEEPPLKDVGNGHKVACFFPGELKR
CCCCCCCCCHHHHHHCCCCCCHHHCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]