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The map label for this gene is thiI [H]

Identifier: 239617818

GI number: 239617818

Start: 1543864

End: 1545078

Strand: Reverse

Name: thiI [H]

Synonym: Kole_1444

Alternate gene names: 239617818

Gene position: 1545078-1543864 (Counterclockwise)

Preceding gene: 239617846

Following gene: 239617817

Centisome position: 67.12

GC content: 41.56

Gene sequence:

>1215_bases
ATGAAAAAAATCATCATCATACGATACGGTGAAATAGGATTAAAAGGCAAAAACAGAAGCTATTTCGAAAAAGCGCTCAT
CAGAAACTTAAAATCCCAGATTCCCGGTGCAAAAGTTTATAACAAGAAGAAAAGATTCCTGATCGATATTCCTGAGGAAT
TCATATCTGAAGCTATGGAAATTGTTTCCAGGACATTTGGTGTTCAAAATTACAGTCTTGGTGTTCAAACCAACTTTGAA
ATCTCTGAAATCGAAAAAGTTGCCAGAGAGCTTGTTTCCATGGAGGTAAACACTGGAAAAAGGACCTTCAAAGTAGAGAC
CCGCCGTGCCAATAAACGTTTCCCGATGACAAGCCAGGAGTTGAATGCGTATCTCGGTGAAAAGATACTGGATGCTTTTC
CGGAATTGAAGGTAGATGTCCATACTCCGGATTTCAAAATAGGTGTTGAAGTTAGAAACGAAGGAGTTCTTGTTTTTGGG
GACAAGATTCCTGGTCCCGGTGGTCTTCCTGTCGGAGTCAGCGGATCAGCATTACTGCTGCTTTCGGGAGGTATTGACAG
TCCCGTTGCTGGATGGTATGCCCTAAAGAGAGGGATCACCCTTCACGCCATTCATTTCGCAAGTCCACCTTACACCGGTG
AAAAAGCCTTTGAAAAAGTCCTTGATCTCGCAAAAGCGTTGACAAAATACAATGGCGGTAGGGACATGTTGCTGTACCGG
GTCCATTTTACAAAATTACAGCTGGCTATTCACCGCAACGTCCGAGAAAGCCTTAGTCTCGTTATACAACGACGCGCTAT
GATGAGGATTGCCACAAAAATAGCAAAAGAACATGGTTACAAAGCGATAGTTACGGGTGAGAATCTTGGACAGGTAGCCA
GCCAAACAATAGAAAATCTTCACACTATCGGAAGCGCAACTGATATACTTGTTTTAAGGCCTTTAACAGGATTCGATAAG
ATCGAAACAATACAGATTGCGAAAAAAATCGAGACTTTTGAAATCTCGATACAACCATACGAAGACTGCTGCACCGTTTT
CGTTCCACAACAACCAGCCACAAAAGCAAGAATCGTTGACGTTGAAATCGAAGAGTCGAAACTCGATTTAGCTCCATTAT
TGGATGAAACAATGAAGATGGTAGAAATTTATAAACTAAGGCAAGGAAAGGTGATAGAAATTGAAAAGCCGGGAATGGAT
AAAACAAATTCGTGA

Upstream 100 bases:

>100_bases
AAGTATTCCCATTAAAAGAATCTTTTTCATTTTCATCCCCCCTACTTCGTAAGTTGAGTTAAAATAATTATATCATTGTA
CTATCCAGGAGGCCTAAGAA

Downstream 100 bases:

>100_bases
TTTTTTTCTGACAATCTGGGTAGCGATAGCCGTTACAGGTTATGTGTTGGTATATGGTGCTTTCGTTCTTCTCATCAGCT
GGTTCATTGAAAAGTTTCGA

Product: thiamine biosynthesis/tRNA modification protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKKIIIIRYGEIGLKGKNRSYFEKALIRNLKSQIPGAKVYNKKKRFLIDIPEEFISEAMEIVSRTFGVQNYSLGVQTNFE
ISEIEKVARELVSMEVNTGKRTFKVETRRANKRFPMTSQELNAYLGEKILDAFPELKVDVHTPDFKIGVEVRNEGVLVFG
DKIPGPGGLPVGVSGSALLLLSGGIDSPVAGWYALKRGITLHAIHFASPPYTGEKAFEKVLDLAKALTKYNGGRDMLLYR
VHFTKLQLAIHRNVRESLSLVIQRRAMMRIATKIAKEHGYKAIVTGENLGQVASQTIENLHTIGSATDILVLRPLTGFDK
IETIQIAKKIETFEISIQPYEDCCTVFVPQQPATKARIVDVEIEESKLDLAPLLDETMKMVEIYKLRQGKVIEIEKPGMD
KTNS

Sequences:

>Translated_404_residues
MKKIIIIRYGEIGLKGKNRSYFEKALIRNLKSQIPGAKVYNKKKRFLIDIPEEFISEAMEIVSRTFGVQNYSLGVQTNFE
ISEIEKVARELVSMEVNTGKRTFKVETRRANKRFPMTSQELNAYLGEKILDAFPELKVDVHTPDFKIGVEVRNEGVLVFG
DKIPGPGGLPVGVSGSALLLLSGGIDSPVAGWYALKRGITLHAIHFASPPYTGEKAFEKVLDLAKALTKYNGGRDMLLYR
VHFTKLQLAIHRNVRESLSLVIQRRAMMRIATKIAKEHGYKAIVTGENLGQVASQTIENLHTIGSATDILVLRPLTGFDK
IETIQIAKKIETFEISIQPYEDCCTVFVPQQPATKARIVDVEIEESKLDLAPLLDETMKMVEIYKLRQGKVIEIEKPGMD
KTNS
>Mature_404_residues
MKKIIIIRYGEIGLKGKNRSYFEKALIRNLKSQIPGAKVYNKKKRFLIDIPEEFISEAMEIVSRTFGVQNYSLGVQTNFE
ISEIEKVARELVSMEVNTGKRTFKVETRRANKRFPMTSQELNAYLGEKILDAFPELKVDVHTPDFKIGVEVRNEGVLVFG
DKIPGPGGLPVGVSGSALLLLSGGIDSPVAGWYALKRGITLHAIHFASPPYTGEKAFEKVLDLAKALTKYNGGRDMLLYR
VHFTKLQLAIHRNVRESLSLVIQRRAMMRIATKIAKEHGYKAIVTGENLGQVASQTIENLHTIGSATDILVLRPLTGFDK
IETIQIAKKIETFEISIQPYEDCCTVFVPQQPATKARIVDVEIEESKLDLAPLLDETMKMVEIYKLRQGKVIEIEKPGMD
KTNS

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=288, Percent_Identity=29.1666666666667, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 45293; Mature: 45293

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIIIRYGEIGLKGKNRSYFEKALIRNLKSQIPGAKVYNKKKRFLIDIPEEFISEAME
CCEEEEEEECCEECCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEECCHHHHHHHHH
IVSRTFGVQNYSLGVQTNFEISEIEKVARELVSMEVNTGKRTFKVETRRANKRFPMTSQE
HHHHHHCCCEEEECEECCCCHHHHHHHHHHHHHEECCCCCEEEEEEECCCCCCCCCCHHH
LNAYLGEKILDAFPELKVDVHTPDFKIGVEVRNEGVLVFGDKIPGPGGLPVGVSGSALLL
HHHHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCEEEECCCCCCCCCCCCCCCCCEEEE
LSGGIDSPVAGWYALKRGITLHAIHFASPPYTGEKAFEKVLDLAKALTKYNGGRDMLLYR
EECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
VHFTKLQLAIHRNVRESLSLVIQRRAMMRIATKIAKEHGYKAIVTGENLGQVASQTIENL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
HTIGSATDILVLRPLTGFDKIETIQIAKKIETFEISIQPYEDCCTVFVPQQPATKARIVD
HHCCCCCCEEEEECCCCCCHHHHHHHHHHEEEEEEEECCCCCCEEEEECCCCCCCEEEEE
VEIEESKLDLAPLLDETMKMVEIYKLRQGKVIEIEKPGMDKTNS
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MKKIIIIRYGEIGLKGKNRSYFEKALIRNLKSQIPGAKVYNKKKRFLIDIPEEFISEAME
CCEEEEEEECCEECCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEECCHHHHHHHHH
IVSRTFGVQNYSLGVQTNFEISEIEKVARELVSMEVNTGKRTFKVETRRANKRFPMTSQE
HHHHHHCCCEEEECEECCCCHHHHHHHHHHHHHEECCCCCEEEEEEECCCCCCCCCCHHH
LNAYLGEKILDAFPELKVDVHTPDFKIGVEVRNEGVLVFGDKIPGPGGLPVGVSGSALLL
HHHHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCEEEECCCCCCCCCCCCCCCCCEEEE
LSGGIDSPVAGWYALKRGITLHAIHFASPPYTGEKAFEKVLDLAKALTKYNGGRDMLLYR
EECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
VHFTKLQLAIHRNVRESLSLVIQRRAMMRIATKIAKEHGYKAIVTGENLGQVASQTIENL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
HTIGSATDILVLRPLTGFDKIETIQIAKKIETFEISIQPYEDCCTVFVPQQPATKARIVD
HHCCCCCCEEEEECCCCCCHHHHHHHHHHEEEEEEEECCCCCCEEEEECCCCCCCEEEEE
VEIEESKLDLAPLLDETMKMVEIYKLRQGKVIEIEKPGMDKTNS
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]