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The map label for this gene is pyk [H]

Identifier: 239617309

GI number: 239617309

Start: 971724

End: 972680

Strand: Reverse

Name: pyk [H]

Synonym: Kole_0918

Alternate gene names: 239617309

Gene position: 972680-971724 (Counterclockwise)

Preceding gene: 239617311

Following gene: 239617308

Centisome position: 42.25

GC content: 38.56

Gene sequence:

>957_bases
TTGAATTTTAAAATTTTTGCTACTATAAAGGACTTTTCAATTGCCCGAGAGCTGCTGAATGCTGGAGTAACTGCTTTCAG
GGTTAATTCTTCACATATGGAGATTGGTGAGTTAATAGAGTTTTTAGAAGAATACCAGTTCCATTTCTACAACATCCCGC
TCTATATCGATCTTAAAGGTAGCAAAAAAAGACTTCGAAAAGATCAACGAACCGTTGAACTTATTCCGGGGGAAAAGATT
GTTTTTGCTACAAGTGAAAGCCCGGAAATAAAAACCCTTTTAGTAGAACCTGCTGTATTGAAACTTCTTTCCCCGAACCT
GAATGTTTCCCTAAATGATGGAAAGATAAAGTTGAAGATTCTCGATAACGATGGAAATAGAGCCTATGCAATAGTACTGA
AAGCTGGAAGAGTCCATGGAGCTAAGGGAATAAACTTTGAATTCCTTTCGGATAAAAATTTCAGATCTTTTTCTTTAACC
GAGAAGGACGTGGAAATAATCTCAAAAACACGTGATTATCCCTTTGTGAGATACGCATTATCCTTTGTTAGTTCTCCTGA
ACAGATAAAAAATCTAAGAGCAATTTCACGAAGGTATGTGGCTGCTAAGATAGAAAATTATTTGGATTTGGATAAACTGA
AGGAAATTTCAAGTGAAACCGACGAAATGTGGTTTTGTCGGGGTGATCTTGGAGCACATCTTGGAATGAGGGGACTTGCA
AAATTCTATGGTGAATTTGTGAAATCTATGCGGGATTTTACCTGCCCTGTCTTCATGGCAGGTGAGGTTATGGAACACAT
GGTAGACAATCCCCGACCAACGAGAAGCGAAATTTGTCACCTTTATGACCTGATAGAACACGGTTTTTCAGGCATAGTAC
TTTCCAATGAAACAGTATCCGGGAATTTCCCAATTGAAGTAGTTAAGGTTGTCAGGGAGGTTATCAGCGGTGATTGA

Upstream 100 bases:

>100_bases
CTCCTTTCAAATCTCGCTTTTTCTTTTTTTTATTATATCACCGGAACATATTGAAGATATGTTCAATCTAAGGGGGTATA
ATTTTTGCGAGGTGATAGAA

Downstream 100 bases:

>100_bases
TTTTACAGTAGAGATAGCGAAAAAACTTGGTTTGCCAACATGGAAGGTCAAAAACTCCATTGAACTCCTTGAAGAAGGAA
AGACAATTCCTTTTATTGCA

Product: Pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNFKIFATIKDFSIARELLNAGVTAFRVNSSHMEIGELIEFLEEYQFHFYNIPLYIDLKGSKKRLRKDQRTVELIPGEKI
VFATSESPEIKTLLVEPAVLKLLSPNLNVSLNDGKIKLKILDNDGNRAYAIVLKAGRVHGAKGINFEFLSDKNFRSFSLT
EKDVEIISKTRDYPFVRYALSFVSSPEQIKNLRAISRRYVAAKIENYLDLDKLKEISSETDEMWFCRGDLGAHLGMRGLA
KFYGEFVKSMRDFTCPVFMAGEVMEHMVDNPRPTRSEICHLYDLIEHGFSGIVLSNETVSGNFPIEVVKVVREVISGD

Sequences:

>Translated_318_residues
MNFKIFATIKDFSIARELLNAGVTAFRVNSSHMEIGELIEFLEEYQFHFYNIPLYIDLKGSKKRLRKDQRTVELIPGEKI
VFATSESPEIKTLLVEPAVLKLLSPNLNVSLNDGKIKLKILDNDGNRAYAIVLKAGRVHGAKGINFEFLSDKNFRSFSLT
EKDVEIISKTRDYPFVRYALSFVSSPEQIKNLRAISRRYVAAKIENYLDLDKLKEISSETDEMWFCRGDLGAHLGMRGLA
KFYGEFVKSMRDFTCPVFMAGEVMEHMVDNPRPTRSEICHLYDLIEHGFSGIVLSNETVSGNFPIEVVKVVREVISGD
>Mature_318_residues
MNFKIFATIKDFSIARELLNAGVTAFRVNSSHMEIGELIEFLEEYQFHFYNIPLYIDLKGSKKRLRKDQRTVELIPGEKI
VFATSESPEIKTLLVEPAVLKLLSPNLNVSLNDGKIKLKILDNDGNRAYAIVLKAGRVHGAKGINFEFLSDKNFRSFSLT
EKDVEIISKTRDYPFVRYALSFVSSPEQIKNLRAISRRYVAAKIENYLDLDKLKEISSETDEMWFCRGDLGAHLGMRGLA
KFYGEFVKSMRDFTCPVFMAGEVMEHMVDNPRPTRSEICHLYDLIEHGFSGIVLSNETVSGNFPIEVVKVVREVISGD

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=348, Percent_Identity=26.1494252873563, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI32967597, Length=348, Percent_Identity=26.1494252873563, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI33286418, Length=225, Percent_Identity=26.2222222222222, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI33286422, Length=225, Percent_Identity=26.2222222222222, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI33286420, Length=225, Percent_Identity=26.2222222222222, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788160, Length=216, Percent_Identity=30.0925925925926, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787965, Length=338, Percent_Identity=26.6272189349112, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17544584, Length=331, Percent_Identity=22.0543806646526, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71984413, Length=258, Percent_Identity=27.5193798449612, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71984406, Length=258, Percent_Identity=27.5193798449612, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17506829, Length=258, Percent_Identity=27.5193798449612, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17506831, Length=258, Percent_Identity=27.5193798449612, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324923, Length=315, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6319279, Length=316, Percent_Identity=25, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648966, Length=281, Percent_Identity=25.6227758007117, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI28571814, Length=340, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648964, Length=340, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646914, Length=139, Percent_Identity=28.0575539568345, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581235, Length=328, Percent_Identity=23.4756097560976, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 36221; Mature: 36221

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKIFATIKDFSIARELLNAGVTAFRVNSSHMEIGELIEFLEEYQFHFYNIPLYIDLKG
CCEEEEEEEHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCEEEECEEEEEECC
SKKRLRKDQRTVELIPGEKIVFATSESPEIKTLLVEPAVLKLLSPNLNVSLNDGKIKLKI
CHHHHHCCCCEEEECCCCEEEEEECCCCCEEEEEECHHHHHHHCCCCEEEECCCEEEEEE
LDNDGNRAYAIVLKAGRVHGAKGINFEFLSDKNFRSFSLTEKDVEIISKTRDYPFVRYAL
EECCCCEEEEEEEEECCCCCCCCCCEEEECCCCCCEECCCHHHHHHHHHCCCCHHHHHHH
SFVSSPEQIKNLRAISRRYVAAKIENYLDLDKLKEISSETDEMWFCRGDLGAHLGMRGLA
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHH
KFYGEFVKSMRDFTCPVFMAGEVMEHMVDNPRPTRSEICHLYDLIEHGFSGIVLSNETVS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCEEC
GNFPIEVVKVVREVISGD
CCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFKIFATIKDFSIARELLNAGVTAFRVNSSHMEIGELIEFLEEYQFHFYNIPLYIDLKG
CCEEEEEEEHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCEEEECEEEEEECC
SKKRLRKDQRTVELIPGEKIVFATSESPEIKTLLVEPAVLKLLSPNLNVSLNDGKIKLKI
CHHHHHCCCCEEEECCCCEEEEEECCCCCEEEEEECHHHHHHHCCCCEEEECCCEEEEEE
LDNDGNRAYAIVLKAGRVHGAKGINFEFLSDKNFRSFSLTEKDVEIISKTRDYPFVRYAL
EECCCCEEEEEEEEECCCCCCCCCCEEEECCCCCCEECCCHHHHHHHHHCCCCHHHHHHH
SFVSSPEQIKNLRAISRRYVAAKIENYLDLDKLKEISSETDEMWFCRGDLGAHLGMRGLA
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHH
KFYGEFVKSMRDFTCPVFMAGEVMEHMVDNPRPTRSEICHLYDLIEHGFSGIVLSNETVS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCEEC
GNFPIEVVKVVREVISGD
CCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA