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The map label for this gene is dnaX [C]

Identifier: 239617286

GI number: 239617286

Start: 947630

End: 948661

Strand: Reverse

Name: dnaX [C]

Synonym: Kole_0895

Alternate gene names: 239617286

Gene position: 948661-947630 (Counterclockwise)

Preceding gene: 239617308

Following gene: 239617285

Centisome position: 41.21

GC content: 38.28

Gene sequence:

>1032_bases
ATGGGAGTTATGAATGAGACCTTTCTAAAAACATTGGAGAACCTTATACATCTGGATAAAGGTATAACGGTAGCGTTTAT
TGGAAAAGCGCCAAGTTTTTTGTTGCAGAGAGCTTTTGAGCTTGTTCGGAGTGATACGAAATGGAATTCGCAACGAGAAA
CCGAGGATCTCCTGGTAGTACAACCATCTTCAGGAAACATAGGAATTGACGATATATTGGATGTTGAACGTTTCTTGCTC
CATAAACCTGCAAATGGCAAGAAAAAATATGTTGTCATTCATAGAGTTGAAAGGCTTACTCCTGAGGCCTCCAATGCTTT
TTTGAAAACACTTGAAGAACCACCGGAATTTGCGACATTGATACTGACAACGACTTCGTGGGAATCTCTTCTTCCAACGA
TAAGGAGCCGGAGCATTTTCTTTGAAATGGATTATCCTTACGAAATGTTAGATGCTCTGAAGAAAAAGTACAGGGAGGAT
GTTAAGTACATTTACGCGGGTTGCTTTGAAGATTTCGAAGTTCTGAAACATTGTCTCGACAATGATGTGTCACAATTGAT
AGAAGCATTGAAGAGCTTTGAGCAATTGAATTTTCAGGATCTGGTGACAAAATTTGAGAAACTTACGGATTCTCGGCCTG
AAGAATATTTGATGCGACGAAAGTTTTATCTTGAGGTTATTCGTAGGATTGTTTCTGAGGGGGAACACGAGTTTTTTAAG
AAATTTGTGGTATTGAAAAAAACTTTGAACACCAACGGTAATTATTTCCATACTCTTCAGGAGATTGCAGCGGTTTTAGG
AACGTTGATTCATGACTCGTTCATTATCAGTAGAACTTCAAGATGGAATCTCATACAGAATTTGGAATTACTCGAATGGT
TGATGGACCTGGATCTTAAAAATACGGCAGAATCTGAGGAAGTTTTCCTGTGGTGTCAAAGGTTATCGAGATTGAAAATT
GGTTCATTGAATAGAGATCTCATATTACACAGAATTCTTTACAGCGCAAGAGAAATTTTTGTGAAACGCTAG

Upstream 100 bases:

>100_bases
ATTTGGTTTCAAATAAACAAATGTTCTCTCCATAAAATCCCTCCCATTTAAAAATATATCATACATATAACATTTGCTAG
CTTTCAGCTATAATTAAATT

Downstream 100 bases:

>100_bases
AAATGGAGGGAAAATATGCCTGAGATATATGGACATGTTTATGGTGTGGAATTCCACGCTGTTGGAAAACTCAGTAAATA
CACCGCAGAAGATTTGCAAT

Product: DNA polymerase III gamma/tau subunits-like protein

Products: diphosphate; DNAn+1

Alternate protein names: DNA Polymerase III Gamma/Tau Subunits-Like Protein; DNA Polymerase III Gamma Subunit-Related Protein; DNA Polymerase III Gamma And Tau; DNA Polymerase III Subunit Delta; DNA Polymerase III Delta Subunit Domain Protein; DNA Polymerase III Gamma/Tau Subunits; DNA Polymerase III Delta Subunit

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MGVMNETFLKTLENLIHLDKGITVAFIGKAPSFLLQRAFELVRSDTKWNSQRETEDLLVVQPSSGNIGIDDILDVERFLL
HKPANGKKKYVVIHRVERLTPEASNAFLKTLEEPPEFATLILTTTSWESLLPTIRSRSIFFEMDYPYEMLDALKKKYRED
VKYIYAGCFEDFEVLKHCLDNDVSQLIEALKSFEQLNFQDLVTKFEKLTDSRPEEYLMRRKFYLEVIRRIVSEGEHEFFK
KFVVLKKTLNTNGNYFHTLQEIAAVLGTLIHDSFIISRTSRWNLIQNLELLEWLMDLDLKNTAESEEVFLWCQRLSRLKI
GSLNRDLILHRILYSAREIFVKR

Sequences:

>Translated_343_residues
MGVMNETFLKTLENLIHLDKGITVAFIGKAPSFLLQRAFELVRSDTKWNSQRETEDLLVVQPSSGNIGIDDILDVERFLL
HKPANGKKKYVVIHRVERLTPEASNAFLKTLEEPPEFATLILTTTSWESLLPTIRSRSIFFEMDYPYEMLDALKKKYRED
VKYIYAGCFEDFEVLKHCLDNDVSQLIEALKSFEQLNFQDLVTKFEKLTDSRPEEYLMRRKFYLEVIRRIVSEGEHEFFK
KFVVLKKTLNTNGNYFHTLQEIAAVLGTLIHDSFIISRTSRWNLIQNLELLEWLMDLDLKNTAESEEVFLWCQRLSRLKI
GSLNRDLILHRILYSAREIFVKR
>Mature_342_residues
GVMNETFLKTLENLIHLDKGITVAFIGKAPSFLLQRAFELVRSDTKWNSQRETEDLLVVQPSSGNIGIDDILDVERFLLH
KPANGKKKYVVIHRVERLTPEASNAFLKTLEEPPEFATLILTTTSWESLLPTIRSRSIFFEMDYPYEMLDALKKKYREDV
KYIYAGCFEDFEVLKHCLDNDVSQLIEALKSFEQLNFQDLVTKFEKLTDSRPEEYLMRRKFYLEVIRRIVSEGEHEFFKK
FVVLKKTLNTNGNYFHTLQEIAAVLGTLIHDSFIISRTSRWNLIQNLELLEWLMDLDLKNTAESEEVFLWCQRLSRLKIG
SLNRDLILHRILYSAREIFVKR

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Tau Chain Serves As A Scaffold To Help In The Dimerizaton Of The Core Co

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 40328; Mature: 40197

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVMNETFLKTLENLIHLDKGITVAFIGKAPSFLLQRAFELVRSDTKWNSQRETEDLLVV
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
QPSSGNIGIDDILDVERFLLHKPANGKKKYVVIHRVERLTPEASNAFLKTLEEPPEFATL
ECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHCCCCCCEEE
ILTTTSWESLLPTIRSRSIFFEMDYPYEMLDALKKKYREDVKYIYAGCFEDFEVLKHCLD
EEEECCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NDVSQLIEALKSFEQLNFQDLVTKFEKLTDSRPEEYLMRRKFYLEVIRRIVSEGEHEFFK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KFVVLKKTLNTNGNYFHTLQEIAAVLGTLIHDSFIISRTSRWNLIQNLELLEWLMDLDLK
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
NTAESEEVFLWCQRLSRLKIGSLNRDLILHRILYSAREIFVKR
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GVMNETFLKTLENLIHLDKGITVAFIGKAPSFLLQRAFELVRSDTKWNSQRETEDLLVV
CCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
QPSSGNIGIDDILDVERFLLHKPANGKKKYVVIHRVERLTPEASNAFLKTLEEPPEFATL
ECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHCCCCCCEEE
ILTTTSWESLLPTIRSRSIFFEMDYPYEMLDALKKKYREDVKYIYAGCFEDFEVLKHCLD
EEEECCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NDVSQLIEALKSFEQLNFQDLVTKFEKLTDSRPEEYLMRRKFYLEVIRRIVSEGEHEFFK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KFVVLKKTLNTNGNYFHTLQEIAAVLGTLIHDSFIISRTSRWNLIQNLELLEWLMDLDLK
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
NTAESEEVFLWCQRLSRLKIGSLNRDLILHRILYSAREIFVKR
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA