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The map label for this gene is rsmE [H]

Identifier: 239617245

GI number: 239617245

Start: 901510

End: 902205

Strand: Reverse

Name: rsmE [H]

Synonym: Kole_0853

Alternate gene names: 239617245

Gene position: 902205-901510 (Counterclockwise)

Preceding gene: 239617246

Following gene: 239617244

Centisome position: 39.19

GC content: 41.24

Gene sequence:

>696_bases
GTGCCAAACGCTTTCTTTGTCGAAAGGGTTGAAGAAAAACGACTTATATTTGATGAGATTGAAGCTCATCATATGAAGGT
TGTTCGTGTAAAACCAGGTGATGAGCTTGTTGCCACTGATGGTAAAGGCACACTCTATGAGTTTGTTCTCCAGGAGCTTT
CAAAGAATTACTCTGTCGGACGAATGCTATCAAAAAAGGTCTTCCATCACGACGATAGAACTGTTATCGTCTCTGTGGCG
TCTATGAAATGGCTAAGGCTCCGTTTGATTTTAGAAAAAAGTACGGAGCTTGGTGCGGATCGAATAGAGATCTTCAATTG
TGAGAGGACCGTAGCCAGAGTTGATCAAAGAAAAGTTGAAAAATTTCGTAGAATAGTGAAAGAAGCAGCCAAGCAAAGTG
TTAATCCCTTTTTACCTGAGGTAGTTATCCACGAAAAACTGCCACGGTTCAATTCGGATGATTTGAATATTTTCCTGGAC
TTTAAGAAAGAGAGCATCTCTACCTTGCAAGAGAAAATAAGAGGGTCGAATGTTGTTCGGATAATAGTAGGACCTGAAGG
TGGTTTCACCAAAGAGGAAATCCGTGAGATGCAGCAAGACTCCATTTCCGTTTCTCTTGGGTCGAGAATCCTTCGAACGG
AGACGGCTGTGATAGCTTCTTTAAGTTTCGTAAATTTCTCTATTGGAAGAATGTGA

Upstream 100 bases:

>100_bases
GACTCTGGTTGCCATCGTTGAGAACTATCAGCTTCCGGATGGAAGGATAAGAGTTCCAGAAGTCCTGGTTCCTTATATGG
GAACGGAGGTAATAGGCTGA

Downstream 100 bases:

>100_bases
AGAAGTCAGGGGGTGAAATAATGGGAGAAAAGAAGTTCTTTTCTCATGTTAAAGAATTAAAAGAGATCGAAAGTGAATAC
AGGGAAAAGCTCATGGAAGC

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MPNAFFVERVEEKRLIFDEIEAHHMKVVRVKPGDELVATDGKGTLYEFVLQELSKNYSVGRMLSKKVFHHDDRTVIVSVA
SMKWLRLRLILEKSTELGADRIEIFNCERTVARVDQRKVEKFRRIVKEAAKQSVNPFLPEVVIHEKLPRFNSDDLNIFLD
FKKESISTLQEKIRGSNVVRIIVGPEGGFTKEEIREMQQDSISVSLGSRILRTETAVIASLSFVNFSIGRM

Sequences:

>Translated_231_residues
MPNAFFVERVEEKRLIFDEIEAHHMKVVRVKPGDELVATDGKGTLYEFVLQELSKNYSVGRMLSKKVFHHDDRTVIVSVA
SMKWLRLRLILEKSTELGADRIEIFNCERTVARVDQRKVEKFRRIVKEAAKQSVNPFLPEVVIHEKLPRFNSDDLNIFLD
FKKESISTLQEKIRGSNVVRIIVGPEGGFTKEEIREMQQDSISVSLGSRILRTETAVIASLSFVNFSIGRM
>Mature_230_residues
PNAFFVERVEEKRLIFDEIEAHHMKVVRVKPGDELVATDGKGTLYEFVLQELSKNYSVGRMLSKKVFHHDDRTVIVSVAS
MKWLRLRLILEKSTELGADRIEIFNCERTVARVDQRKVEKFRRIVKEAAKQSVNPFLPEVVIHEKLPRFNSDDLNIFLDF
KKESISTLQEKIRGSNVVRIIVGPEGGFTKEEIREMQQDSISVSLGSRILRTETAVIASLSFVNFSIGRM

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=224, Percent_Identity=23.2142857142857, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26579; Mature: 26448

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNAFFVERVEEKRLIFDEIEAHHMKVVRVKPGDELVATDGKGTLYEFVLQELSKNYSVG
CCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHCCCCHH
RMLSKKVFHHDDRTVIVSVASMKWLRLRLILEKSTELGADRIEIFNCERTVARVDQRKVE
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHH
KFRRIVKEAAKQSVNPFLPEVVIHEKLPRFNSDDLNIFLDFKKESISTLQEKIRGSNVVR
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHCCCCEEE
IIVGPEGGFTKEEIREMQQDSISVSLGSRILRTETAVIASLSFVNFSIGRM
EEECCCCCCCHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PNAFFVERVEEKRLIFDEIEAHHMKVVRVKPGDELVATDGKGTLYEFVLQELSKNYSVG
CCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHCCCCHH
RMLSKKVFHHDDRTVIVSVASMKWLRLRLILEKSTELGADRIEIFNCERTVARVDQRKVE
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHH
KFRRIVKEAAKQSVNPFLPEVVIHEKLPRFNSDDLNIFLDFKKESISTLQEKIRGSNVVR
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHCCCCEEE
IIVGPEGGFTKEEIREMQQDSISVSLGSRILRTETAVIASLSFVNFSIGRM
EEECCCCCCCHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]