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The map label for this gene is pncA [H]

Identifier: 239617199

GI number: 239617199

Start: 857674

End: 858201

Strand: Reverse

Name: pncA [H]

Synonym: Kole_0806

Alternate gene names: 239617199

Gene position: 858201-857674 (Counterclockwise)

Preceding gene: 239617200

Following gene: 239617198

Centisome position: 37.28

GC content: 41.67

Gene sequence:

>528_bases
ATCAAAAGTTTACTCCTTATCGACCTTCAGAAAGATTTTGTCGAACCGGGAGGAGCACTCTTTGTTGATGGTTCCAGAAA
GATTTTTCCGGTGGTGCTTGAACTGATAGATCGGTTCAAGAGGCAGGGTTTACCGATAATCACCACCATGGATTTTCATG
AGAAGGATGACCCGGAATTTGAGTACTGGCCCCCTCATTGTGTTAAAGGGACAAAAGGAGTAGAACTTGCTGACGTCGTA
AAACGTGAGCTTATAGGTTATGAAAAGCATTATACGATAAAGAAAAGGAAATACAGTGCTTTTTATCAGACCAAATTCGA
TGCGTTAATCAGGGAACTTGATCTGGATGAATTTCATGTAGCAGGCGTGGTGACCAATATATGTGTGCTCTTCACTGTCG
AAGAGCTGAGGAACAGGAAACTTAAAGTGGTTTTGTATGAAAAAGGCGTGATCTCCTATGACAACGATCTTCACAGATTT
GCTTTGAGGCAGATGAAAGAGGTGCTCAATGTAGAGGTGATTGAATGA

Upstream 100 bases:

>100_bases
ATAAACTGAAAGCACAGTGCATGCGGGGCGAATGCCCCGCTTTTTAATTTGTATTGAAGTGTTAGGGATTCTGCGATAGA
ATTGTTTCAGGGGTGGTATT

Downstream 100 bases:

>100_bases
AAAAAAGATTAGATCCGCGGGTATTCAAGGTACCAATTGACAAAATAAGAGCTGGTTACTATTCAGATAAATATTTTACC
CGGTATGTCGAGGTTTTGAA

Product: isochorismatase hydrolase

Products: nicotinate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKSLLLIDLQKDFVEPGGALFVDGSRKIFPVVLELIDRFKRQGLPIITTMDFHEKDDPEFEYWPPHCVKGTKGVELADVV
KRELIGYEKHYTIKKRKYSAFYQTKFDALIRELDLDEFHVAGVVTNICVLFTVEELRNRKLKVVLYEKGVISYDNDLHRF
ALRQMKEVLNVEVIE

Sequences:

>Translated_175_residues
MKSLLLIDLQKDFVEPGGALFVDGSRKIFPVVLELIDRFKRQGLPIITTMDFHEKDDPEFEYWPPHCVKGTKGVELADVV
KRELIGYEKHYTIKKRKYSAFYQTKFDALIRELDLDEFHVAGVVTNICVLFTVEELRNRKLKVVLYEKGVISYDNDLHRF
ALRQMKEVLNVEVIE
>Mature_175_residues
MKSLLLIDLQKDFVEPGGALFVDGSRKIFPVVLELIDRFKRQGLPIITTMDFHEKDDPEFEYWPPHCVKGTKGVELADVV
KRELIGYEKHYTIKKRKYSAFYQTKFDALIRELDLDEFHVAGVVTNICVLFTVEELRNRKLKVVLYEKGVISYDNDLHRF
ALRQMKEVLNVEVIE

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.5.1.19

Molecular weight: Translated: 20493; Mature: 20493

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLLLIDLQKDFVEPGGALFVDGSRKIFPVVLELIDRFKRQGLPIITTMDFHEKDDPEF
CCCEEEEECCHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
EYWPPHCVKGTKGVELADVVKRELIGYEKHYTIKKRKYSAFYQTKFDALIRELDLDEFHV
CCCCCHHCCCCCCCHHHHHHHHHHHCCHHCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHH
AGVVTNICVLFTVEELRNRKLKVVLYEKGVISYDNDLHRFALRQMKEVLNVEVIE
HHHHHHHHHHEEHHHHHCCCEEEEEEECCEECCCCHHHHHHHHHHHHHHCCEECC
>Mature Secondary Structure
MKSLLLIDLQKDFVEPGGALFVDGSRKIFPVVLELIDRFKRQGLPIITTMDFHEKDDPEF
CCCEEEEECCHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
EYWPPHCVKGTKGVELADVVKRELIGYEKHYTIKKRKYSAFYQTKFDALIRELDLDEFHV
CCCCCHHCCCCCCCHHHHHHHHHHHCCHHCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHH
AGVVTNICVLFTVEELRNRKLKVVLYEKGVISYDNDLHRFALRQMKEVLNVEVIE
HHHHHHHHHHEEHHHHHCCCEEEEEEECCEECCCCHHHHHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nicotinamide; H2O

Specific reaction: nicotinamide + H2O = nicotinate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]