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The map label for this gene is ctfB [H]

Identifier: 239616996

GI number: 239616996

Start: 644587

End: 645246

Strand: Reverse

Name: ctfB [H]

Synonym: Kole_0596

Alternate gene names: 239616996

Gene position: 645246-644587 (Counterclockwise)

Preceding gene: 239616997

Following gene: 239616995

Centisome position: 28.03

GC content: 46.21

Gene sequence:

>660_bases
ATGATGAATCCTAAGGAATTAATAGCAAGGCGTGTGGCACTTGAATTGAAAGATGGAAATCTCGCTAATCTTGGTATAGG
CATTCCAACACTGGTTGCGAATTATATTCCTGAAGGAATACAGGTTTTTTTCCAATCTGAGAACGGAATTATTGGGATGG
GGCCCGTTCCAGAACCGGGTATGGAAAACCAAGATCTTACCGACGCGGGCGGACAATACGTAACAGCCCTCCCCGGGGCT
ATGACTTTCGATAGTGCAATGTCTTTTGGGCTTATACGGGGAGGTCATCTTGACGTGACTGTCCTTGGTGCTCTCCAAGT
AGATGAAAAGGGTATGCTCGCCAACTGGATGATTCCTGGCAAAATGGTTCCCGGTATGGGGGGAGCAATGGATCTCGTTA
CCGGGGCCAAACGTGTTATCGTTGCTATGACACACACAGCTAAAGGAAGGCCAAAATTGGTTAAGAGATGCACTCTGCCG
TTAACTTCCGTTAGACCCGTCGATCTTGTGGTAACGGATCTAGCAGTTATCGAACCTACAGAAGATGGACTGTTATTGAA
AGAAGTTGCTCCCGGTGTTACAGTTGATCAGGTGCTTGAACAGACAGAGGCAAAATTGATCATTCCGGATAACGTCAGTG
AGATGCCAATAAAAGTTTAA

Upstream 100 bases:

>100_bases
TAGTGGAAGTGGAGGAAGTTGTTCCTGTAGGTGCTATTCCGCCTGATGAAGTGCACATCCCTGGGGTACTTGTAGATTAT
ATTGTCGTTGGGGGGTGTAA

Downstream 100 bases:

>100_bases
ACGCGTCAAGAATGAAAAGGGGCCTCCAGGCCCTTTTTTATTATATAGGCTTTCACTGAAAGAAAAGTTCATTTTAACCT
GGTTCTACGTGTTAGAATGA

Product: 3-oxoacid CoA-transferase, B subunit

Products: NA

Alternate protein names: Coat B; Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit B [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MMNPKELIARRVALELKDGNLANLGIGIPTLVANYIPEGIQVFFQSENGIIGMGPVPEPGMENQDLTDAGGQYVTALPGA
MTFDSAMSFGLIRGGHLDVTVLGALQVDEKGMLANWMIPGKMVPGMGGAMDLVTGAKRVIVAMTHTAKGRPKLVKRCTLP
LTSVRPVDLVVTDLAVIEPTEDGLLLKEVAPGVTVDQVLEQTEAKLIIPDNVSEMPIKV

Sequences:

>Translated_219_residues
MMNPKELIARRVALELKDGNLANLGIGIPTLVANYIPEGIQVFFQSENGIIGMGPVPEPGMENQDLTDAGGQYVTALPGA
MTFDSAMSFGLIRGGHLDVTVLGALQVDEKGMLANWMIPGKMVPGMGGAMDLVTGAKRVIVAMTHTAKGRPKLVKRCTLP
LTSVRPVDLVVTDLAVIEPTEDGLLLKEVAPGVTVDQVLEQTEAKLIIPDNVSEMPIKV
>Mature_219_residues
MMNPKELIARRVALELKDGNLANLGIGIPTLVANYIPEGIQVFFQSENGIIGMGPVPEPGMENQDLTDAGGQYVTALPGA
MTFDSAMSFGLIRGGHLDVTVLGALQVDEKGMLANWMIPGKMVPGMGGAMDLVTGAKRVIVAMTHTAKGRPKLVKRCTLP
LTSVRPVDLVVTDLAVIEPTEDGLLLKEVAPGVTVDQVLEQTEAKLIIPDNVSEMPIKV

Specific function: Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation [H]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=201, Percent_Identity=53.2338308457711, Blast_Score=205, Evalue=3e-53,
Organism=Homo sapiens, GI11545841, Length=216, Percent_Identity=48.1481481481481, Blast_Score=196, Evalue=1e-50,
Organism=Escherichia coli, GI1788552, Length=213, Percent_Identity=55.3990610328638, Blast_Score=240, Evalue=5e-65,
Organism=Escherichia coli, GI1787985, Length=223, Percent_Identity=28.6995515695067, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17531517, Length=214, Percent_Identity=53.2710280373832, Blast_Score=213, Evalue=6e-56,
Organism=Drosophila melanogaster, GI21358325, Length=214, Percent_Identity=51.8691588785047, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24655794, Length=214, Percent_Identity=51.8691588785047, Blast_Score=214, Evalue=5e-56,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.9 [H]

Molecular weight: Translated: 23237; Mature: 23237

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNPKELIARRVALELKDGNLANLGIGIPTLVANYIPEGIQVFFQSENGIIGMGPVPEPG
CCCHHHHHHHHHEEEECCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCC
MENQDLTDAGGQYVTALPGAMTFDSAMSFGLIRGGHLDVTVLGALQVDEKGMLANWMIPG
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHEEECCCEEEEEEEEEEECCCCCEEEEECCC
KMVPGMGGAMDLVTGAKRVIVAMTHTAKGRPKLVKRCTLPLTSVRPVDLVVTDLAVIEPT
CCCCCCCCHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCEEEEEEEEEECCC
EDGLLLKEVAPGVTVDQVLEQTEAKLIIPDNVSEMPIKV
CCCEEHHHHCCCCCHHHHHHHCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MMNPKELIARRVALELKDGNLANLGIGIPTLVANYIPEGIQVFFQSENGIIGMGPVPEPG
CCCHHHHHHHHHEEEECCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCC
MENQDLTDAGGQYVTALPGAMTFDSAMSFGLIRGGHLDVTVLGALQVDEKGMLANWMIPG
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHEEECCCEEEEEEEEEEECCCCCEEEEECCC
KMVPGMGGAMDLVTGAKRVIVAMTHTAKGRPKLVKRCTLPLTSVRPVDLVVTDLAVIEPT
CCCCCCCCHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCEEEEEEEEEECCC
EDGLLLKEVAPGVTVDQVLEQTEAKLIIPDNVSEMPIKV
CCCEEHHHHCCCCCHHHHHHHCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254264; 8226639; 8423010; 11466286; 2383002 [H]