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The map label for this gene is agaR [H]

Identifier: 239616925

GI number: 239616925

Start: 571691

End: 573520

Strand: Reverse

Name: agaR [H]

Synonym: Kole_0522

Alternate gene names: 239616925

Gene position: 573520-571691 (Counterclockwise)

Preceding gene: 239616930

Following gene: 239616924

Centisome position: 24.91

GC content: 41.86

Gene sequence:

>1830_bases
GTGCGGATTCAGATACCGGGAATGAGTGGCATTGAAATCGATGGTCAATTGAAGACGAAAGTTGTTTCGGCTGGACTGGA
ATTCAACTATAAGCTGAATATTCTGGATTCCGGTTATCTAATCAACCTCTCTTTGACAAACATCGAAACGGTTCCTATAA
AACTTTCCAGTTTCACCGTAGCAAAATTTTCTGATCTCGATTTTCCTTTTTATTGCAATAATTGGGGATCATGGTTCCCT
TTCAGGTTGTATAAAAGCATTCCCAACACAAAAGAGGCCGTAGCATTTGCCCAATCTGCAGGGACGACTCTTTTTTCGGC
AACACCAATACCTGAGCTTTTCAATATTGATATATTTCCCAGCGACTATTTTATAGCAAATAAAGGTTTTTTAGCTGGAT
TCCTTGACTCAAAAGTTTCTCATCCATATTTTACGATAGATGAAGCTACCAAAACCGTGGAAGCCAGGATCGAGCTCTTT
GGAAAGCCGCTCCAGCCAGGAGAAACCATAGAGATCGAACCGTTAGTGGTTTTTCATAGTGATAAACTTAACAGAATGCT
GGAAAACTACGCGGAAATGATAAGAGATTACAAAAAACCTGTGTTCAAAGAATTGGAAGGAATCGGCTGGTGCAGCTGGT
ATCACTATTTTACCGATATCACTTTCGAAGAATTGAAGAAAAATGTTAAACTTCTGGCGAATCTTCGCGAAGAAAGAGGG
CTTCCATACACGTTAGTGCAGCTAGACGACGGTTATCAGAAAGACATTGGAGATTGGCTGATAACCAATGAGAAATTTCC
CTCCCTCGAGGAAATAGCAAATGAAATATCAGTGAAAGGTTTCATTCCCGGCTTATGGCTCGCACCTTTCTGTGCCTCGG
AAACCTCGGATCTCTTCCGTGAACATCCGGAATGGTTTGTTAAGGATTCTTCTGGTAATCCCAAACGAGCTTTCAGGAAC
TGGGGAAAAGATATTTACGCTCTCGACCTTTCAAACAGGGAAGTGCTAGGGTTTATAAAAGATCTTATGTCGAAGATCAA
AAAATCAGGATTCAAGTACATCAAAATAGATTTCCTTTTTGCGGGTGCAATACCAGGAATAAGACAGAACGAAAATATAA
CCCCCATAGAAGCATATGTTAAAGGATTGGAGACAATAAGAGGAGCTCTGGGTGATGATGTTTACTTGCTCGGTTGTGGT
GCGCCCTTGCTTCCTTCCGTTGGCCTTGTTGATGCTATGAGAATAGGAGCAGATACCGCACCAATATGGGGTGGAGAGCT
TCCGGATCTGGGTATCCCGAGTGCAAAATACTCAATCCGAAACGCCCTTACGCGTTATTTTATGCATAAGAGGTTATGGA
TAAACGATCCAGATACTCCAATTCTTCGCTCTCAAGCGGCTAAACTCTCTGATAACGAGAAAGAACTTTTCAGTTATGTC
TGTGGGTTACTCGACAATCCCATCATCGAAAGCGATGATATGACTCTTGTGGATGAAGAGGGTATTGAGATCTTACGAAA
GACGCTTTCTTTGAGGGGTGGAAAACCAAGAGTCTTCTTTGACGATACCGGTGAAATCTTTACGGTGGCGGTAAAAGGTG
GAAAAGAAAAAAACTCCGTTGCTGTCATAAACCTCAGTGATGAAAAAGCCAGGGTTAAAATCCCTGAAGACGCCCTTTCA
TGGTCTGGAGTAAATACGGAAGAAATGGATGTAGAGATAGAACCACATTCCATCTGGTTGAGGAGCGTGTCATCAGTAGC
TGTGGAACTAAAAAAAACAGTAGAATACAAAGAAGATGGAAGACAGGTGAATTACTATGAAGAAGCGTGA

Upstream 100 bases:

>100_bases
CAACGGTTTTATCGCCGTATGGCGGCTTTCGTGTGGACGCAACTGCGGTGTAAATAACTTGCGTTATAATCGAGTTGAAA
CGGATTTAGGAGGTATTTAT

Downstream 100 bases:

>100_bases
TTTTTTTGTTATTTCATTGTTTTTTGTATTCTTTCTTCTGTCAGGATGTATTTTTAACCTCTATAAGAGCAAAATTTTTG
GAAGTATTTCTCCTTTTGTT

Product: glycoside hydrolase clan GH-D

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MRIQIPGMSGIEIDGQLKTKVVSAGLEFNYKLNILDSGYLINLSLTNIETVPIKLSSFTVAKFSDLDFPFYCNNWGSWFP
FRLYKSIPNTKEAVAFAQSAGTTLFSATPIPELFNIDIFPSDYFIANKGFLAGFLDSKVSHPYFTIDEATKTVEARIELF
GKPLQPGETIEIEPLVVFHSDKLNRMLENYAEMIRDYKKPVFKELEGIGWCSWYHYFTDITFEELKKNVKLLANLREERG
LPYTLVQLDDGYQKDIGDWLITNEKFPSLEEIANEISVKGFIPGLWLAPFCASETSDLFREHPEWFVKDSSGNPKRAFRN
WGKDIYALDLSNREVLGFIKDLMSKIKKSGFKYIKIDFLFAGAIPGIRQNENITPIEAYVKGLETIRGALGDDVYLLGCG
APLLPSVGLVDAMRIGADTAPIWGGELPDLGIPSAKYSIRNALTRYFMHKRLWINDPDTPILRSQAAKLSDNEKELFSYV
CGLLDNPIIESDDMTLVDEEGIEILRKTLSLRGGKPRVFFDDTGEIFTVAVKGGKEKNSVAVINLSDEKARVKIPEDALS
WSGVNTEEMDVEIEPHSIWLRSVSSVAVELKKTVEYKEDGRQVNYYEEA

Sequences:

>Translated_609_residues
MRIQIPGMSGIEIDGQLKTKVVSAGLEFNYKLNILDSGYLINLSLTNIETVPIKLSSFTVAKFSDLDFPFYCNNWGSWFP
FRLYKSIPNTKEAVAFAQSAGTTLFSATPIPELFNIDIFPSDYFIANKGFLAGFLDSKVSHPYFTIDEATKTVEARIELF
GKPLQPGETIEIEPLVVFHSDKLNRMLENYAEMIRDYKKPVFKELEGIGWCSWYHYFTDITFEELKKNVKLLANLREERG
LPYTLVQLDDGYQKDIGDWLITNEKFPSLEEIANEISVKGFIPGLWLAPFCASETSDLFREHPEWFVKDSSGNPKRAFRN
WGKDIYALDLSNREVLGFIKDLMSKIKKSGFKYIKIDFLFAGAIPGIRQNENITPIEAYVKGLETIRGALGDDVYLLGCG
APLLPSVGLVDAMRIGADTAPIWGGELPDLGIPSAKYSIRNALTRYFMHKRLWINDPDTPILRSQAAKLSDNEKELFSYV
CGLLDNPIIESDDMTLVDEEGIEILRKTLSLRGGKPRVFFDDTGEIFTVAVKGGKEKNSVAVINLSDEKARVKIPEDALS
WSGVNTEEMDVEIEPHSIWLRSVSSVAVELKKTVEYKEDGRQVNYYEEA
>Mature_609_residues
MRIQIPGMSGIEIDGQLKTKVVSAGLEFNYKLNILDSGYLINLSLTNIETVPIKLSSFTVAKFSDLDFPFYCNNWGSWFP
FRLYKSIPNTKEAVAFAQSAGTTLFSATPIPELFNIDIFPSDYFIANKGFLAGFLDSKVSHPYFTIDEATKTVEARIELF
GKPLQPGETIEIEPLVVFHSDKLNRMLENYAEMIRDYKKPVFKELEGIGWCSWYHYFTDITFEELKKNVKLLANLREERG
LPYTLVQLDDGYQKDIGDWLITNEKFPSLEEIANEISVKGFIPGLWLAPFCASETSDLFREHPEWFVKDSSGNPKRAFRN
WGKDIYALDLSNREVLGFIKDLMSKIKKSGFKYIKIDFLFAGAIPGIRQNENITPIEAYVKGLETIRGALGDDVYLLGCG
APLLPSVGLVDAMRIGADTAPIWGGELPDLGIPSAKYSIRNALTRYFMHKRLWINDPDTPILRSQAAKLSDNEKELFSYV
CGLLDNPIIESDDMTLVDEEGIEILRKTLSLRGGKPRVFFDDTGEIFTVAVKGGKEKNSVAVINLSDEKARVKIPEDALS
WSGVNTEEMDVEIEPHSIWLRSVSSVAVELKKTVEYKEDGRQVNYYEEA

Specific function: Alpha-galactosidase associated with the raffinose operon [H]

COG id: COG3345

COG function: function code G; Alpha-galactosidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 36 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002252
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 68774; Mature: 68774

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIQIPGMSGIEIDGQLKTKVVSAGLEFNYKLNILDSGYLINLSLTNIETVPIKLSSFTV
CEEECCCCCCCEECCCHHEEHEECCCEEEEEEEEEECCEEEEEEECCEEEEEEEECCEEE
AKFSDLDFPFYCNNWGSWFPFRLYKSIPNTKEAVAFAQSAGTTLFSATPIPELFNIDIFP
EEECCCCCCEEECCCCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCHHEEEEECC
SDYFIANKGFLAGFLDSKVSHPYFTIDEATKTVEARIELFGKPLQPGETIEIEPLVVFHS
CCEEEECCCCEEEHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEC
DKLNRMLENYAEMIRDYKKPVFKELEGIGWCSWYHYFTDITFEELKKNVKLLANLREERG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
LPYTLVQLDDGYQKDIGDWLITNEKFPSLEEIANEISVKGFIPGLWLAPFCASETSDLFR
CCEEEEEECCCHHHCCCCEEEECCCCCCHHHHHHHHEECCCCCCHHHHHHHCCCHHHHHH
EHPEWFVKDSSGNPKRAFRNWGKDIYALDLSNREVLGFIKDLMSKIKKSGFKYIKIDFLF
HCCCEEEECCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEEE
AGAIPGIRQNENITPIEAYVKGLETIRGALGDDVYLLGCGAPLLPSVGLVDAMRIGADTA
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCC
PIWGGELPDLGIPSAKYSIRNALTRYFMHKRLWINDPDTPILRSQAAKLSDNEKELFSYV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCHHHHHHHH
CGLLDNPIIESDDMTLVDEEGIEILRKTLSLRGGKPRVFFDDTGEIFTVAVKGGKEKNSV
HHHHCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCCCCCE
AVINLSDEKARVKIPEDALSWSGVNTEEMDVEIEPHSIWLRSVSSVAVELKKTVEYKEDG
EEEEECCCCCEEECCHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
RQVNYYEEA
CEEEEEECC
>Mature Secondary Structure
MRIQIPGMSGIEIDGQLKTKVVSAGLEFNYKLNILDSGYLINLSLTNIETVPIKLSSFTV
CEEECCCCCCCEECCCHHEEHEECCCEEEEEEEEEECCEEEEEEECCEEEEEEEECCEEE
AKFSDLDFPFYCNNWGSWFPFRLYKSIPNTKEAVAFAQSAGTTLFSATPIPELFNIDIFP
EEECCCCCCEEECCCCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCHHEEEEECC
SDYFIANKGFLAGFLDSKVSHPYFTIDEATKTVEARIELFGKPLQPGETIEIEPLVVFHS
CCEEEECCCCEEEHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEC
DKLNRMLENYAEMIRDYKKPVFKELEGIGWCSWYHYFTDITFEELKKNVKLLANLREERG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
LPYTLVQLDDGYQKDIGDWLITNEKFPSLEEIANEISVKGFIPGLWLAPFCASETSDLFR
CCEEEEEECCCHHHCCCCEEEECCCCCCHHHHHHHHEECCCCCCHHHHHHHCCCHHHHHH
EHPEWFVKDSSGNPKRAFRNWGKDIYALDLSNREVLGFIKDLMSKIKKSGFKYIKIDFLF
HCCCEEEECCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEEE
AGAIPGIRQNENITPIEAYVKGLETIRGALGDDVYLLGCGAPLLPSVGLVDAMRIGADTA
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCC
PIWGGELPDLGIPSAKYSIRNALTRYFMHKRLWINDPDTPILRSQAAKLSDNEKELFSYV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCHHHHHHHH
CGLLDNPIIESDDMTLVDEEGIEILRKTLSLRGGKPRVFFDDTGEIFTVAVKGGKEKNSV
HHHHCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCCCCCE
AVINLSDEKARVKIPEDALSWSGVNTEEMDVEIEPHSIWLRSVSSVAVELKKTVEYKEDG
EEEEECCCCCEEECCHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
RQVNYYEEA
CEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA