Click here to switch to the map view.

The map label for this gene is epsE [H]

Identifier: 239616762

GI number: 239616762

Start: 373328

End: 375016

Strand: Direct

Name: epsE [H]

Synonym: Kole_0355

Alternate gene names: 239616762

Gene position: 373328-375016 (Clockwise)

Preceding gene: 239616761

Following gene: 239616763

Centisome position: 16.22

GC content: 43.28

Gene sequence:

>1689_bases
ATGAGGGTTTACAAACGCCTGGGTGAGCTTTTAACAGAGCGGGGCTTGATTACTCCTGAGGTTCTTCAGCAGGCCGTAAC
AATACAAAAAAAGGTTGGCAAACCCCTCGGTGAAATACTCGTAGGGATGGGATTGCTATCCTGGGAGGATATCTACACCG
CTTTATCCAAACAGTACAACCTGGAACTCGTTGAAGAACTTCCCAATATGGTACCGCCTGAGTTATTGAAACTTGTTCCA
CGTTCTGTGGCAGAACGCCTTAAGGTGGTTCCAATTGAGTTTTCCCCGGAAACCAACACGCTCAAAGTCGTTACTCCGGA
TGTTTTGAAAGTCCCTCAGATTGAACGAGAACTCTCTTTCCTTACAGGAAACAAAATCAAGGTAGCACTTACTCCCCCAC
CAAATTTTGAGGCACTGTATAGAGCAAGTTATGAAGAAGCATCTTCAAGTGAAATTATTGAACACACCTTCGATTTAGAA
CCTTCAGAAGAAGTGGTCGAAGAAGAAGAGGTTACCAGTGAAGAAACACCTGTAGGAAAGTTCATAAACGCCCTTCTCGA
AAACGCGATAAGAAGTGATGCAAGTGATATTCACCTCGAACCTTTTGAAAAGGTGGCAGTGGGAAGATTCAGGATAGATG
GAATTTTAAGGAAGATACTGACCTATCCTAGACGTGCCCATAACTCGGTGGTTTCAAGAATAAAAGTCATGTGTGGTCTT
GATATATCAGAAAAGAGGCTTCCACAGGACGGTAAGTTCTTCATTCGAAGGGGTGGAGAACAGTACGATTTCCGTGTATC
CACAATGCCAACGATCTTTGGCGAAAAAGTCGTCATGAGAGTTTTGAGGGTTTCCAACGCAAAAAAGAAACTCGAAGAGC
TTGGATTAAGCGATTACAATTTAAAGAGGTTTAGAAAGCTTCTGGAAGCTCCCCACGGAATAATATTGGTTTCCGGACCC
ACAGGTAGTGGTAAATCCACGACGTTGGTGGCAGTCTTAAACGAAGTAACATCTGAAAAGGTGAATGTTGTTACAGCGGA
AGATCCTGTTGAATATACAATAGAGGGTATAACTCAGTGTCAGGTAAACGCTGAAATTGGTTTAACTTTTGCGCGCTACT
TGCGTGCTTTTCTTAGGCAAGACCCGGATGTTATAATGGTAGGTGAAATCCGTGATAGAGAAACAGCGCAACTCGCCATA
GAAGCTTCGCTTACAGGTCATCTTGTCTTCTCCACGATTCATACCAATAGTGCTCCTGGAGCTGTCGCAAGATTGGTCAA
CATGGGGGTTGACCCATTTCTTTTAAGCGCTTCTTTGATTGGGGTAATTGGCCAAAGACTGGTCAGAAGACTTTGTAGTA
ATTGTAAAGTAAAAATCCCGATAAGAGAAGAAATCCTGGAAATAGCAAGCAAAATTTTCCCAAATAAAAATGAATTCTAC
GAATTCGTACCCGGTTCTGGATGTAACGAATGCCGGGGAATTGGATACAAAGGAAGAACCGGTATTCATGAGGTTCTTAT
TGTCAATAACGAACTGAGGGATTTAATGGTTAGAGGTGCTTCTGAACACGAACTTAGTGAAGCTGCAAAAGCGGCCGGGA
TGAGAACCCTTTATGAGGACGGTATCGAAAAGGTCCTGCAGGGCATAACATCAGTCGAAGAAGTAAATAGAGTCGCAACC
GAACTGTAA

Upstream 100 bases:

>100_bases
CCGGTCTTGAAATGGAAGAAAGCGAACTGTTTGGTGAAGCTTCCGATATACCTGCTATTTATAGATTCGGTCTGGATACC
CATGAAGAAGAGGAAGGTAC

Downstream 100 bases:

>100_bases
ACGTGTGAGCATACTAAAACATAAATTTGAAGGAACATCATAACCGGCTTAATTGGCTTTAGAATGAAATTACCGCTCCA
TGAGCGGTAATTTTTTTTCT

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MRVYKRLGELLTERGLITPEVLQQAVTIQKKVGKPLGEILVGMGLLSWEDIYTALSKQYNLELVEELPNMVPPELLKLVP
RSVAERLKVVPIEFSPETNTLKVVTPDVLKVPQIERELSFLTGNKIKVALTPPPNFEALYRASYEEASSSEIIEHTFDLE
PSEEVVEEEEVTSEETPVGKFINALLENAIRSDASDIHLEPFEKVAVGRFRIDGILRKILTYPRRAHNSVVSRIKVMCGL
DISEKRLPQDGKFFIRRGGEQYDFRVSTMPTIFGEKVVMRVLRVSNAKKKLEELGLSDYNLKRFRKLLEAPHGIILVSGP
TGSGKSTTLVAVLNEVTSEKVNVVTAEDPVEYTIEGITQCQVNAEIGLTFARYLRAFLRQDPDVIMVGEIRDRETAQLAI
EASLTGHLVFSTIHTNSAPGAVARLVNMGVDPFLLSASLIGVIGQRLVRRLCSNCKVKIPIREEILEIASKIFPNKNEFY
EFVPGSGCNECRGIGYKGRTGIHEVLIVNNELRDLMVRGASEHELSEAAKAAGMRTLYEDGIEKVLQGITSVEEVNRVAT
EL

Sequences:

>Translated_562_residues
MRVYKRLGELLTERGLITPEVLQQAVTIQKKVGKPLGEILVGMGLLSWEDIYTALSKQYNLELVEELPNMVPPELLKLVP
RSVAERLKVVPIEFSPETNTLKVVTPDVLKVPQIERELSFLTGNKIKVALTPPPNFEALYRASYEEASSSEIIEHTFDLE
PSEEVVEEEEVTSEETPVGKFINALLENAIRSDASDIHLEPFEKVAVGRFRIDGILRKILTYPRRAHNSVVSRIKVMCGL
DISEKRLPQDGKFFIRRGGEQYDFRVSTMPTIFGEKVVMRVLRVSNAKKKLEELGLSDYNLKRFRKLLEAPHGIILVSGP
TGSGKSTTLVAVLNEVTSEKVNVVTAEDPVEYTIEGITQCQVNAEIGLTFARYLRAFLRQDPDVIMVGEIRDRETAQLAI
EASLTGHLVFSTIHTNSAPGAVARLVNMGVDPFLLSASLIGVIGQRLVRRLCSNCKVKIPIREEILEIASKIFPNKNEFY
EFVPGSGCNECRGIGYKGRTGIHEVLIVNNELRDLMVRGASEHELSEAAKAAGMRTLYEDGIEKVLQGITSVEEVNRVAT
EL
>Mature_562_residues
MRVYKRLGELLTERGLITPEVLQQAVTIQKKVGKPLGEILVGMGLLSWEDIYTALSKQYNLELVEELPNMVPPELLKLVP
RSVAERLKVVPIEFSPETNTLKVVTPDVLKVPQIERELSFLTGNKIKVALTPPPNFEALYRASYEEASSSEIIEHTFDLE
PSEEVVEEEEVTSEETPVGKFINALLENAIRSDASDIHLEPFEKVAVGRFRIDGILRKILTYPRRAHNSVVSRIKVMCGL
DISEKRLPQDGKFFIRRGGEQYDFRVSTMPTIFGEKVVMRVLRVSNAKKKLEELGLSDYNLKRFRKLLEAPHGIILVSGP
TGSGKSTTLVAVLNEVTSEKVNVVTAEDPVEYTIEGITQCQVNAEIGLTFARYLRAFLRQDPDVIMVGEIRDRETAQLAI
EASLTGHLVFSTIHTNSAPGAVARLVNMGVDPFLLSASLIGVIGQRLVRRLCSNCKVKIPIREEILEIASKIFPNKNEFY
EFVPGSGCNECRGIGYKGRTGIHEVLIVNNELRDLMVRGASEHELSEAAKAAGMRTLYEDGIEKVLQGITSVEEVNRVAT
EL

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=439, Percent_Identity=46.0136674259681, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1786296, Length=388, Percent_Identity=41.7525773195876, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI87082188, Length=200, Percent_Identity=41.5, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 62633; Mature: 62633

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVYKRLGELLTERGLITPEVLQQAVTIQKKVGKPLGEILVGMGLLSWEDIYTALSKQYN
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHCC
LELVEELPNMVPPELLKLVPRSVAERLKVVPIEFSPETNTLKVVTPDVLKVPQIERELSF
HHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCHHCCCHHHHHHHH
LTGNKIKVALTPPPNFEALYRASYEEASSSEIIEHTFDLEPSEEVVEEEEVTSEETPVGK
HCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHH
FINALLENAIRSDASDIHLEPFEKVAVGRFRIDGILRKILTYPRRAHNSVVSRIKVMCGL
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
DISEKRLPQDGKFFIRRGGEQYDFRVSTMPTIFGEKVVMRVLRVSNAKKKLEELGLSDYN
CCHHHHCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LKRFRKLLEAPHGIILVSGPTGSGKSTTLVAVLNEVTSEKVNVVTAEDPVEYTIEGITQC
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHCHHEE
QVNAEIGLTFARYLRAFLRQDPDVIMVGEIRDRETAQLAIEASLTGHLVFSTIHTNSAPG
EECHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHEEEEEECCCCEEEEEEEECCCCCH
AVARLVNMGVDPFLLSASLIGVIGQRLVRRLCSNCKVKIPIREEILEIASKIFPNKNEFY
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCCCCE
EFVPGSGCNECRGIGYKGRTGIHEVLIVNNELRDLMVRGASEHELSEAAKAAGMRTLYED
EECCCCCCHHHCCCCCCCCCCCEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GIEKVLQGITSVEEVNRVATEL
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVYKRLGELLTERGLITPEVLQQAVTIQKKVGKPLGEILVGMGLLSWEDIYTALSKQYN
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHCC
LELVEELPNMVPPELLKLVPRSVAERLKVVPIEFSPETNTLKVVTPDVLKVPQIERELSF
HHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCHHCCCHHHHHHHH
LTGNKIKVALTPPPNFEALYRASYEEASSSEIIEHTFDLEPSEEVVEEEEVTSEETPVGK
HCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHH
FINALLENAIRSDASDIHLEPFEKVAVGRFRIDGILRKILTYPRRAHNSVVSRIKVMCGL
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
DISEKRLPQDGKFFIRRGGEQYDFRVSTMPTIFGEKVVMRVLRVSNAKKKLEELGLSDYN
CCHHHHCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LKRFRKLLEAPHGIILVSGPTGSGKSTTLVAVLNEVTSEKVNVVTAEDPVEYTIEGITQC
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHCHHEE
QVNAEIGLTFARYLRAFLRQDPDVIMVGEIRDRETAQLAIEASLTGHLVFSTIHTNSAPG
EECHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHEEEEEECCCCEEEEEEEECCCCCH
AVARLVNMGVDPFLLSASLIGVIGQRLVRRLCSNCKVKIPIREEILEIASKIFPNKNEFY
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCCCCE
EFVPGSGCNECRGIGYKGRTGIHEVLIVNNELRDLMVRGASEHELSEAAKAAGMRTLYED
EECCCCCCHHHCCCCCCCCCCCEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GIEKVLQGITSVEEVNRVATEL
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]