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The map label for this gene is gyrB [H]

Identifier: 239616758

GI number: 239616758

Start: 368458

End: 370353

Strand: Direct

Name: gyrB [H]

Synonym: Kole_0351

Alternate gene names: 239616758

Gene position: 368458-370353 (Clockwise)

Preceding gene: 239616757

Following gene: 239616759

Centisome position: 16.01

GC content: 39.5

Gene sequence:

>1896_bases
ATGTCTACTGATTACAATGCGCAAAGTATTAAGATCTTAAAGGGGTTGGAACCGGTTAGACAGAGACCGGGGATGTATAT
TGGTTCCACAGGAAAAGCGGGACTTCATCATTTAATATATGAGGTTGTTGATAATAGTATAGACGAAGTAGTTGGAGGTT
ATTGTGATTATATTAAAATAACGATCTTTGAAGATGGAAGCGTCTCAGTTTGGGACAATGGTAGAGGAATCCCTGTCGAC
ATTCATCCCGAAACAGGTACAAGCGCTCTCGAAGTTGTTATGACCACCCTTCATGCTGGAGGTAAGTTCTCAAAAGACAG
CTATAAAATAAGTGGTGGATTACACGGTGTCGGTGTCTCAGTGGTGAATGCTCTTTCAGAATGGATGGAGGTAAAGGTCT
ATAGAGATGGAAAAATCTACCGCCAGCGCTATGAAAGAGGAAAACCGACGACTCCTGTTGAGATCATAGGTGAAACGGAT
AAGAGAGGATCACTGGTTGCTTTTAAACCAGATAGCCTGGTGTTCACAACTACAGATTTCGATTTTGATATCCTCGAATC
CCGTTTTAAAGAACTTGCATTTTTAAATCCCAGAGTTACCATAGAATTCGAAGATAGGCGATACGACGAGAAAAGGGTTT
TCCATTTTGAAGGCGGTATTGTAGAATTCGTTAAATATCTCAACAGGAAAAAGACAGCAATAAATAAAGAGCCCTTCTAT
ATTGAAGGAACATACAATGATGTGAAGATCCAGCTTGCTATGCAATACACAAATGCTTACGATGAAAATATCCTGACCTT
TGTAAACAACATTAAAACCGTAGAAGGTGGAACACACCTAACTGGCTTCAAGACCGTTTTGACAAAAATGATGAATGATT
ATGCAAGAAAATTGAACGTGTTGAAAGACAAAGATCCAAACCTTCAGGGTGAAGATGTAAGAGAAGGATTAACGGCCGTT
TTGAGTGTGTTCGTCAAAGAACCACAATTTGAAGGCCAGACAAAAGCTAAATTAGGAAATGAAGAAGTATACGAGGCTGT
TATCAAGGTTTTAAGAGATAAACTTCCAGAGGTTTTTGACTACCATACGAAAGAGCTCAAAGCCATAATCATCAAAGCAC
TCGACGCGGCAAAAGCCAGAGTAGCTGCAAAAAAAGCGAGAGAAATGATCAGACGAAAGAATGCGCTTGAAAACACAACT
CTTCCCGGAAAGCTTGCAGACTGTACTTCAACGGATCTCGAAACTACTGAACTGTTTATAGTGGAAGGAGATTCTGCTGG
AGGATCTGCAAAGCAGGCGCGCGACCGGGAATTCCAAGCTATTTTGCCGTTAAGAGGAAAGATTCTCAACGTAGAAAAAT
CGAGCTTTGAAAAGCTTCTTAAGAATGAACAGATAAATAATATAATCGTGGCTATCGGAACCGGAATAGGAGATGACTGT
AAACTTGAAAACCTGAGGTATGGAAAGATTATAATAATGACAGATGCCGATGTTGACGGTGCACACATCAGAACATTACT
TCTCACCCTTTTCTATCGATACATGACTCCATTGATAAACGCAGGTCATATCTATATAGCTCAAGCACCTCTATATAAGG
TTGAACTTAACAGAAAGAAGTATTACTTCTATTCAGATGAAGAATTGGAAGAATTTCTCAAAAATAACGACGGCAAGAAG
GTATCGATACAGAGATACAAAGGTCTTGGAGAAATGAATCCAGACCAACTCTGGGAAACTACAATGAATCCCGAATCCAG
AAAATTGATAAAGATAGAAATGGAAGATGTAGAAGAAGCCGATAGAGTCTTTACTATTCTGATGGGAAGCGAGGTAGAAG
AAAGAAGAGAATTCATTGAAAGGCATGCTTTGATGATAACCGAACTTGATGTATGA

Upstream 100 bases:

>100_bases
AAAAGGAGATTCTCAAAAGAATCCATGAAGAACTACACGGAGATTTCGTAAGAAAGATTATTTTTATCCCAAAAAAGTCA
AAGTAACTGGAGGTGCATCT

Downstream 100 bases:

>100_bases
ACTTAGAAGCGAGAGGTTTTATACTTCTTTTAATTCTAGCGGTAGTTTGGACCATTGGGTTAATAAATTTTGTGGTGGAA
ATCTACTTTACCAGAGCTCA

Product: DNA gyrase, B subunit

Products: NA

Alternate protein names: Topoisomerase II subunit B [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MSTDYNAQSIKILKGLEPVRQRPGMYIGSTGKAGLHHLIYEVVDNSIDEVVGGYCDYIKITIFEDGSVSVWDNGRGIPVD
IHPETGTSALEVVMTTLHAGGKFSKDSYKISGGLHGVGVSVVNALSEWMEVKVYRDGKIYRQRYERGKPTTPVEIIGETD
KRGSLVAFKPDSLVFTTTDFDFDILESRFKELAFLNPRVTIEFEDRRYDEKRVFHFEGGIVEFVKYLNRKKTAINKEPFY
IEGTYNDVKIQLAMQYTNAYDENILTFVNNIKTVEGGTHLTGFKTVLTKMMNDYARKLNVLKDKDPNLQGEDVREGLTAV
LSVFVKEPQFEGQTKAKLGNEEVYEAVIKVLRDKLPEVFDYHTKELKAIIIKALDAAKARVAAKKAREMIRRKNALENTT
LPGKLADCTSTDLETTELFIVEGDSAGGSAKQARDREFQAILPLRGKILNVEKSSFEKLLKNEQINNIIVAIGTGIGDDC
KLENLRYGKIIIMTDADVDGAHIRTLLLTLFYRYMTPLINAGHIYIAQAPLYKVELNRKKYYFYSDEELEEFLKNNDGKK
VSIQRYKGLGEMNPDQLWETTMNPESRKLIKIEMEDVEEADRVFTILMGSEVEERREFIERHALMITELDV

Sequences:

>Translated_631_residues
MSTDYNAQSIKILKGLEPVRQRPGMYIGSTGKAGLHHLIYEVVDNSIDEVVGGYCDYIKITIFEDGSVSVWDNGRGIPVD
IHPETGTSALEVVMTTLHAGGKFSKDSYKISGGLHGVGVSVVNALSEWMEVKVYRDGKIYRQRYERGKPTTPVEIIGETD
KRGSLVAFKPDSLVFTTTDFDFDILESRFKELAFLNPRVTIEFEDRRYDEKRVFHFEGGIVEFVKYLNRKKTAINKEPFY
IEGTYNDVKIQLAMQYTNAYDENILTFVNNIKTVEGGTHLTGFKTVLTKMMNDYARKLNVLKDKDPNLQGEDVREGLTAV
LSVFVKEPQFEGQTKAKLGNEEVYEAVIKVLRDKLPEVFDYHTKELKAIIIKALDAAKARVAAKKAREMIRRKNALENTT
LPGKLADCTSTDLETTELFIVEGDSAGGSAKQARDREFQAILPLRGKILNVEKSSFEKLLKNEQINNIIVAIGTGIGDDC
KLENLRYGKIIIMTDADVDGAHIRTLLLTLFYRYMTPLINAGHIYIAQAPLYKVELNRKKYYFYSDEELEEFLKNNDGKK
VSIQRYKGLGEMNPDQLWETTMNPESRKLIKIEMEDVEEADRVFTILMGSEVEERREFIERHALMITELDV
>Mature_630_residues
STDYNAQSIKILKGLEPVRQRPGMYIGSTGKAGLHHLIYEVVDNSIDEVVGGYCDYIKITIFEDGSVSVWDNGRGIPVDI
HPETGTSALEVVMTTLHAGGKFSKDSYKISGGLHGVGVSVVNALSEWMEVKVYRDGKIYRQRYERGKPTTPVEIIGETDK
RGSLVAFKPDSLVFTTTDFDFDILESRFKELAFLNPRVTIEFEDRRYDEKRVFHFEGGIVEFVKYLNRKKTAINKEPFYI
EGTYNDVKIQLAMQYTNAYDENILTFVNNIKTVEGGTHLTGFKTVLTKMMNDYARKLNVLKDKDPNLQGEDVREGLTAVL
SVFVKEPQFEGQTKAKLGNEEVYEAVIKVLRDKLPEVFDYHTKELKAIIIKALDAAKARVAAKKAREMIRRKNALENTTL
PGKLADCTSTDLETTELFIVEGDSAGGSAKQARDREFQAILPLRGKILNVEKSSFEKLLKNEQINNIIVAIGTGIGDDCK
LENLRYGKIIIMTDADVDGAHIRTLLLTLFYRYMTPLINAGHIYIAQAPLYKVELNRKKYYFYSDEELEEFLKNNDGKKV
SIQRYKGLGEMNPDQLWETTMNPESRKLIKIEMEDVEEADRVFTILMGSEVEERREFIERHALMITELDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=620, Percent_Identity=25.1612903225806, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI19913408, Length=619, Percent_Identity=25.3634894991922, Blast_Score=138, Evalue=2e-32,
Organism=Escherichia coli, GI48994957, Length=555, Percent_Identity=52.0720720720721, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI1789408, Length=634, Percent_Identity=40.3785488958991, Blast_Score=465, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI212645845, Length=601, Percent_Identity=25.7903494176373, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17535065, Length=615, Percent_Identity=24.390243902439, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI212645657, Length=176, Percent_Identity=29.5454545454545, Blast_Score=76, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=630, Percent_Identity=24.6031746031746, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17136538, Length=577, Percent_Identity=26.6897746967071, Blast_Score=137, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71720; Mature: 71589

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDYNAQSIKILKGLEPVRQRPGMYIGSTGKAGLHHLIYEVVDNSIDEVVGGYCDYIKI
CCCCCCCCHHHHHHCCCHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHHHCCCCCEEEE
TIFEDGSVSVWDNGRGIPVDIHPETGTSALEVVMTTLHAGGKFSKDSYKISGGLHGVGVS
EEEECCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSEWMEVKVYRDGKIYRQRYERGKPTTPVEIIGETDKRGSLVAFKPDSLVFTTTDF
HHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCEEEEECCC
DFDILESRFKELAFLNPRVTIEFEDRRYDEKRVFHFEGGIVEFVKYLNRKKTAINKEPFY
CHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEE
IEGTYNDVKIQLAMQYTNAYDENILTFVNNIKTVEGGTHLTGFKTVLTKMMNDYARKLNV
EEECCCCEEEEEEEEECCCCHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHH
LKDKDPNLQGEDVREGLTAVLSVFVKEPQFEGQTKAKLGNEEVYEAVIKVLRDKLPEVFD
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCHHHHH
YHTKELKAIIIKALDAAKARVAAKKAREMIRRKNALENTTLPGKLADCTSTDLETTELFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
VEGDSAGGSAKQARDREFQAILPLRGKILNVEKSSFEKLLKNEQINNIIVAIGTGIGDDC
EECCCCCCCHHHHHCCCCEEECCCCCEEEECCHHHHHHHHCCCCCCEEEEEEECCCCCCC
KLENLRYGKIIIMTDADVDGAHIRTLLLTLFYRYMTPLINAGHIYIAQAPLYKVELNRKK
EECCEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCEE
YYFYSDEELEEFLKNNDGKKVSIQRYKGLGEMNPDQLWETTMNPESRKLIKIEMEDVEEA
EEEECHHHHHHHHHCCCCCEEEEHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEHHHHHHH
DRVFTILMGSEVEERREFIERHALMITELDV
HHEEEEEECCCHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure 
STDYNAQSIKILKGLEPVRQRPGMYIGSTGKAGLHHLIYEVVDNSIDEVVGGYCDYIKI
CCCCCCCHHHHHHCCCHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHHHCCCCCEEEE
TIFEDGSVSVWDNGRGIPVDIHPETGTSALEVVMTTLHAGGKFSKDSYKISGGLHGVGVS
EEEECCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSEWMEVKVYRDGKIYRQRYERGKPTTPVEIIGETDKRGSLVAFKPDSLVFTTTDF
HHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCEEEEECCC
DFDILESRFKELAFLNPRVTIEFEDRRYDEKRVFHFEGGIVEFVKYLNRKKTAINKEPFY
CHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEE
IEGTYNDVKIQLAMQYTNAYDENILTFVNNIKTVEGGTHLTGFKTVLTKMMNDYARKLNV
EEECCCCEEEEEEEEECCCCHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHH
LKDKDPNLQGEDVREGLTAVLSVFVKEPQFEGQTKAKLGNEEVYEAVIKVLRDKLPEVFD
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCHHHHH
YHTKELKAIIIKALDAAKARVAAKKAREMIRRKNALENTTLPGKLADCTSTDLETTELFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
VEGDSAGGSAKQARDREFQAILPLRGKILNVEKSSFEKLLKNEQINNIIVAIGTGIGDDC
EECCCCCCCHHHHHCCCCEEECCCCCEEEECCHHHHHHHHCCCCCCEEEEEEECCCCCCC
KLENLRYGKIIIMTDADVDGAHIRTLLLTLFYRYMTPLINAGHIYIAQAPLYKVELNRKK
EECCEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCEE
YYFYSDEELEEFLKNNDGKKVSIQRYKGLGEMNPDQLWETTMNPESRKLIKIEMEDVEEA
EEEECHHHHHHHHHCCCCCEEEEHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEHHHHHHH
DRVFTILMGSEVEERREFIERHALMITELDV
HHEEEEEECCCHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8863738; 10360571 [H]